Diversity of “ Ca . Micrarchaeota” in Two Distinct Types of Acidic Environments and Their Associations with Thermoplasmatales
“ Candidatus Micrarchaeota” are widely distributed in acidic environments; however, their cultivability and our understanding of their interactions with potential hosts are very limited. Their habitats were so far attributed with acidic sites, soils, peats, freshwater systems, and hypersaline mats....
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MDPI AG
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ftdoajarticles:oai:doaj.org/article:7eccc7ada5b84b66a32471eb85e7e6dd 2023-05-15T16:58:59+02:00 Diversity of “ Ca . Micrarchaeota” in Two Distinct Types of Acidic Environments and Their Associations with Thermoplasmatales Olga V. Golyshina Rafael Bargiela Stepan V. Toshchakov Nikolay A. Chernyh Soshila Ramayah Aleksei A. Korzhenkov Ilya V. Kublanov Peter N. Golyshin 2019-06-01T00:00:00Z https://doi.org/10.3390/genes10060461 https://doaj.org/article/7eccc7ada5b84b66a32471eb85e7e6dd EN eng MDPI AG https://www.mdpi.com/2073-4425/10/6/461 https://doaj.org/toc/2073-4425 2073-4425 doi:10.3390/genes10060461 https://doaj.org/article/7eccc7ada5b84b66a32471eb85e7e6dd Genes, Vol 10, Iss 6, p 461 (2019) “ Ca . Mancarchaeum acidiphilum” ARMAN-2 “ Ca . Micrarchaeota” DPANN superphylum Thermoplasmatales Cuniculiplasma acidic environments acid mine drainage sites terrestrial hot springs Genetics QH426-470 article 2019 ftdoajarticles https://doi.org/10.3390/genes10060461 2022-12-31T02:42:05Z “ Candidatus Micrarchaeota” are widely distributed in acidic environments; however, their cultivability and our understanding of their interactions with potential hosts are very limited. Their habitats were so far attributed with acidic sites, soils, peats, freshwater systems, and hypersaline mats. Using cultivation and culture-independent approaches (16S rRNA gene clonal libraries, high-throughput amplicon sequencing of V3-V4 region of 16S rRNA genes), we surveyed the occurrence of these archaea in geothermal areas on Kamchatka Peninsula and Kunashir Island and assessed their taxonomic diversity in relation with another type of low-pH environment, acid mine drainage stream (Wales, UK). We detected “ Ca . Micrarchaeota” in thermophilic heterotrophic enrichment cultures of Kunashir and Kamchatka that appeared as two different phylotypes, namely “ Ca . Mancarchaeum acidiphilum”-, and ARMAN-2-related, alongside their potential hosts, Cuniculiplasma spp. and other Thermoplasmatales archaea without defined taxonomic position. These clusters of “ Ca . Micrarchaeota” together with three other groups were also present in mesophilic acid mine drainage community. Present work expands our knowledge on the diversity of “ Ca . Micrarchaeota” in thermophilic and mesophilic acidic environments, suggests cultivability patterns of acidophilic archaea and establishes potential links between low-abundance species of thermophilic “ Ca . Micrarchaeota” and certain Thermoplasmatales , such as Cuniculiplasma spp. in situ . Article in Journal/Newspaper Kamchatka Kamchatka Peninsula Directory of Open Access Journals: DOAJ Articles Kamchatka Peninsula ENVELOPE(160.000,160.000,56.000,56.000) Arman’ ENVELOPE(150.141,150.141,59.662,59.662) Genes 10 6 461 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
“ Ca . Mancarchaeum acidiphilum” ARMAN-2 “ Ca . Micrarchaeota” DPANN superphylum Thermoplasmatales Cuniculiplasma acidic environments acid mine drainage sites terrestrial hot springs Genetics QH426-470 |
spellingShingle |
“ Ca . Mancarchaeum acidiphilum” ARMAN-2 “ Ca . Micrarchaeota” DPANN superphylum Thermoplasmatales Cuniculiplasma acidic environments acid mine drainage sites terrestrial hot springs Genetics QH426-470 Olga V. Golyshina Rafael Bargiela Stepan V. Toshchakov Nikolay A. Chernyh Soshila Ramayah Aleksei A. Korzhenkov Ilya V. Kublanov Peter N. Golyshin Diversity of “ Ca . Micrarchaeota” in Two Distinct Types of Acidic Environments and Their Associations with Thermoplasmatales |
topic_facet |
“ Ca . Mancarchaeum acidiphilum” ARMAN-2 “ Ca . Micrarchaeota” DPANN superphylum Thermoplasmatales Cuniculiplasma acidic environments acid mine drainage sites terrestrial hot springs Genetics QH426-470 |
description |
“ Candidatus Micrarchaeota” are widely distributed in acidic environments; however, their cultivability and our understanding of their interactions with potential hosts are very limited. Their habitats were so far attributed with acidic sites, soils, peats, freshwater systems, and hypersaline mats. Using cultivation and culture-independent approaches (16S rRNA gene clonal libraries, high-throughput amplicon sequencing of V3-V4 region of 16S rRNA genes), we surveyed the occurrence of these archaea in geothermal areas on Kamchatka Peninsula and Kunashir Island and assessed their taxonomic diversity in relation with another type of low-pH environment, acid mine drainage stream (Wales, UK). We detected “ Ca . Micrarchaeota” in thermophilic heterotrophic enrichment cultures of Kunashir and Kamchatka that appeared as two different phylotypes, namely “ Ca . Mancarchaeum acidiphilum”-, and ARMAN-2-related, alongside their potential hosts, Cuniculiplasma spp. and other Thermoplasmatales archaea without defined taxonomic position. These clusters of “ Ca . Micrarchaeota” together with three other groups were also present in mesophilic acid mine drainage community. Present work expands our knowledge on the diversity of “ Ca . Micrarchaeota” in thermophilic and mesophilic acidic environments, suggests cultivability patterns of acidophilic archaea and establishes potential links between low-abundance species of thermophilic “ Ca . Micrarchaeota” and certain Thermoplasmatales , such as Cuniculiplasma spp. in situ . |
format |
Article in Journal/Newspaper |
author |
Olga V. Golyshina Rafael Bargiela Stepan V. Toshchakov Nikolay A. Chernyh Soshila Ramayah Aleksei A. Korzhenkov Ilya V. Kublanov Peter N. Golyshin |
author_facet |
Olga V. Golyshina Rafael Bargiela Stepan V. Toshchakov Nikolay A. Chernyh Soshila Ramayah Aleksei A. Korzhenkov Ilya V. Kublanov Peter N. Golyshin |
author_sort |
Olga V. Golyshina |
title |
Diversity of “ Ca . Micrarchaeota” in Two Distinct Types of Acidic Environments and Their Associations with Thermoplasmatales |
title_short |
Diversity of “ Ca . Micrarchaeota” in Two Distinct Types of Acidic Environments and Their Associations with Thermoplasmatales |
title_full |
Diversity of “ Ca . Micrarchaeota” in Two Distinct Types of Acidic Environments and Their Associations with Thermoplasmatales |
title_fullStr |
Diversity of “ Ca . Micrarchaeota” in Two Distinct Types of Acidic Environments and Their Associations with Thermoplasmatales |
title_full_unstemmed |
Diversity of “ Ca . Micrarchaeota” in Two Distinct Types of Acidic Environments and Their Associations with Thermoplasmatales |
title_sort |
diversity of “ ca . micrarchaeota” in two distinct types of acidic environments and their associations with thermoplasmatales |
publisher |
MDPI AG |
publishDate |
2019 |
url |
https://doi.org/10.3390/genes10060461 https://doaj.org/article/7eccc7ada5b84b66a32471eb85e7e6dd |
long_lat |
ENVELOPE(160.000,160.000,56.000,56.000) ENVELOPE(150.141,150.141,59.662,59.662) |
geographic |
Kamchatka Peninsula Arman’ |
geographic_facet |
Kamchatka Peninsula Arman’ |
genre |
Kamchatka Kamchatka Peninsula |
genre_facet |
Kamchatka Kamchatka Peninsula |
op_source |
Genes, Vol 10, Iss 6, p 461 (2019) |
op_relation |
https://www.mdpi.com/2073-4425/10/6/461 https://doaj.org/toc/2073-4425 2073-4425 doi:10.3390/genes10060461 https://doaj.org/article/7eccc7ada5b84b66a32471eb85e7e6dd |
op_doi |
https://doi.org/10.3390/genes10060461 |
container_title |
Genes |
container_volume |
10 |
container_issue |
6 |
container_start_page |
461 |
_version_ |
1766051115608047616 |