Systematic identification and characterization of long noncoding RNAs (lncRNAs) during Aedes albopictus development.
Background Aedes albopictus originated in the tropical forests of Southeast Asia and can currently be found on all continents. As one of the main arboviral vectors, the control of Ae. albopictus requires novel strategies, informed by a deep knowledge of its biology. Little is known regarding mosquit...
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ftdoajarticles:oai:doaj.org/article:7ec6d46a701f4efc835bfba250bbfabb 2023-05-15T15:09:53+02:00 Systematic identification and characterization of long noncoding RNAs (lncRNAs) during Aedes albopictus development. Wenjuan Liu Peng Cheng Kexin Zhang Maoqing Gong Zhong Zhang Ruiling Zhang 2022-04-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0010245 https://doaj.org/article/7ec6d46a701f4efc835bfba250bbfabb EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0010245 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010245 https://doaj.org/article/7ec6d46a701f4efc835bfba250bbfabb PLoS Neglected Tropical Diseases, Vol 16, Iss 4, p e0010245 (2022) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2022 ftdoajarticles https://doi.org/10.1371/journal.pntd.0010245 2022-12-31T02:34:52Z Background Aedes albopictus originated in the tropical forests of Southeast Asia and can currently be found on all continents. As one of the main arboviral vectors, the control of Ae. albopictus requires novel strategies, informed by a deep knowledge of its biology. Little is known regarding mosquito long noncoding RNAs (lncRNAs), which are transcripts longer than 200 nucleotides that lack protein-coding potential and have roles in developmental regulation. Results Based on RNA-seq data from five developmental time points, eggs, early larvae, late larvae, pupae, and adults (female and male) of Ae. albopictus, 21,414 lncRNAs were characterized in this study. Differential expression analysis revealed that lncRNAs exhibited developmental stage specificity. The expression of most lncRNAs was upregulated at the onset of metamorphosis developmental stages. More differentially expressed lncRNAs were observed between eggs and early larvae. Weighted gene co-expression network analysis (WGCNA) further confirmed that the expression patterns of lncRNAs were obviously correlated with specific developmental time points. Functional annotation using co-expression analysis revealed that lncRNAs may be involved in the regulation of metamorphic developmental transitions of Ae. albopictus. The hub lncRNAs and hub gene clusters were identified for each module that were highly associated with specific developmental time points. Conclusions The results of this study will facilitate future researches to elucidate the regulatory mechanisms of lncRNAs in the development of Ae. albopictus and utilize lncRNAs to assist with mosquito control. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 16 4 e0010245 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Wenjuan Liu Peng Cheng Kexin Zhang Maoqing Gong Zhong Zhang Ruiling Zhang Systematic identification and characterization of long noncoding RNAs (lncRNAs) during Aedes albopictus development. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Background Aedes albopictus originated in the tropical forests of Southeast Asia and can currently be found on all continents. As one of the main arboviral vectors, the control of Ae. albopictus requires novel strategies, informed by a deep knowledge of its biology. Little is known regarding mosquito long noncoding RNAs (lncRNAs), which are transcripts longer than 200 nucleotides that lack protein-coding potential and have roles in developmental regulation. Results Based on RNA-seq data from five developmental time points, eggs, early larvae, late larvae, pupae, and adults (female and male) of Ae. albopictus, 21,414 lncRNAs were characterized in this study. Differential expression analysis revealed that lncRNAs exhibited developmental stage specificity. The expression of most lncRNAs was upregulated at the onset of metamorphosis developmental stages. More differentially expressed lncRNAs were observed between eggs and early larvae. Weighted gene co-expression network analysis (WGCNA) further confirmed that the expression patterns of lncRNAs were obviously correlated with specific developmental time points. Functional annotation using co-expression analysis revealed that lncRNAs may be involved in the regulation of metamorphic developmental transitions of Ae. albopictus. The hub lncRNAs and hub gene clusters were identified for each module that were highly associated with specific developmental time points. Conclusions The results of this study will facilitate future researches to elucidate the regulatory mechanisms of lncRNAs in the development of Ae. albopictus and utilize lncRNAs to assist with mosquito control. |
format |
Article in Journal/Newspaper |
author |
Wenjuan Liu Peng Cheng Kexin Zhang Maoqing Gong Zhong Zhang Ruiling Zhang |
author_facet |
Wenjuan Liu Peng Cheng Kexin Zhang Maoqing Gong Zhong Zhang Ruiling Zhang |
author_sort |
Wenjuan Liu |
title |
Systematic identification and characterization of long noncoding RNAs (lncRNAs) during Aedes albopictus development. |
title_short |
Systematic identification and characterization of long noncoding RNAs (lncRNAs) during Aedes albopictus development. |
title_full |
Systematic identification and characterization of long noncoding RNAs (lncRNAs) during Aedes albopictus development. |
title_fullStr |
Systematic identification and characterization of long noncoding RNAs (lncRNAs) during Aedes albopictus development. |
title_full_unstemmed |
Systematic identification and characterization of long noncoding RNAs (lncRNAs) during Aedes albopictus development. |
title_sort |
systematic identification and characterization of long noncoding rnas (lncrnas) during aedes albopictus development. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2022 |
url |
https://doi.org/10.1371/journal.pntd.0010245 https://doaj.org/article/7ec6d46a701f4efc835bfba250bbfabb |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 16, Iss 4, p e0010245 (2022) |
op_relation |
https://doi.org/10.1371/journal.pntd.0010245 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010245 https://doaj.org/article/7ec6d46a701f4efc835bfba250bbfabb |
op_doi |
https://doi.org/10.1371/journal.pntd.0010245 |
container_title |
PLOS Neglected Tropical Diseases |
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16 |
container_issue |
4 |
container_start_page |
e0010245 |
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1766340977010671616 |