The Trypanosoma cruzi Protein TcHTE Is Critical for Heme Uptake.
Trypanosoma cruzi, the etiological agent of Chagas' disease, presents nutritional requirements for several metabolites. It requires heme for the biosynthesis of several heme-proteins involved in essential metabolic pathways like mitochondrial cytochromes and respiratory complexes, as well as en...
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ftdoajarticles:oai:doaj.org/article:7eab0ba16f8d4e25ba1d751bed3ca43f 2023-05-15T15:08:23+02:00 The Trypanosoma cruzi Protein TcHTE Is Critical for Heme Uptake. Marcelo L Merli Lucas Pagura Josefina Hernández María Julia Barisón Elizabeth M F Pral Ariel M Silber Julia A Cricco 2016-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0004359 https://doaj.org/article/7eab0ba16f8d4e25ba1d751bed3ca43f EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4713871?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004359 https://doaj.org/article/7eab0ba16f8d4e25ba1d751bed3ca43f PLoS Neglected Tropical Diseases, Vol 10, Iss 1, p e0004359 (2016) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2016 ftdoajarticles https://doi.org/10.1371/journal.pntd.0004359 2022-12-31T05:52:05Z Trypanosoma cruzi, the etiological agent of Chagas' disease, presents nutritional requirements for several metabolites. It requires heme for the biosynthesis of several heme-proteins involved in essential metabolic pathways like mitochondrial cytochromes and respiratory complexes, as well as enzymes involved in the biosynthesis of sterols and unsaturated fatty acids. However, this parasite lacks a complete route for its synthesis. In view of these facts, T. cruzi has to incorporate heme from the environment during its life cycle. In other words, their hosts must supply the heme for heme-protein synthesis. Although the acquisition of heme is a fundamental issue for the parasite's replication and survival, how this cofactor is imported and distributed is poorly understood. In this work, we used different fluorescent heme analogs to explore heme uptake along the different life-cycle stages of T. cruzi, showing that this parasite imports it during its replicative stages: the epimastigote in the insect vector and the intracellular amastigote in the mammalian host. Also, we identified and characterized a T. cruzi protein (TcHTE) with 55% of sequence similarity to LHR1 (protein involved in L. amazonensis heme transport), which is located in the flagellar pocket, where the transport of nutrients proceeds in trypanosomatids. We postulate TcHTE as a protein involved in improving the efficiency of the heme uptake or trafficking in T. cruzi. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 10 1 e0004359 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Marcelo L Merli Lucas Pagura Josefina Hernández María Julia Barisón Elizabeth M F Pral Ariel M Silber Julia A Cricco The Trypanosoma cruzi Protein TcHTE Is Critical for Heme Uptake. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Trypanosoma cruzi, the etiological agent of Chagas' disease, presents nutritional requirements for several metabolites. It requires heme for the biosynthesis of several heme-proteins involved in essential metabolic pathways like mitochondrial cytochromes and respiratory complexes, as well as enzymes involved in the biosynthesis of sterols and unsaturated fatty acids. However, this parasite lacks a complete route for its synthesis. In view of these facts, T. cruzi has to incorporate heme from the environment during its life cycle. In other words, their hosts must supply the heme for heme-protein synthesis. Although the acquisition of heme is a fundamental issue for the parasite's replication and survival, how this cofactor is imported and distributed is poorly understood. In this work, we used different fluorescent heme analogs to explore heme uptake along the different life-cycle stages of T. cruzi, showing that this parasite imports it during its replicative stages: the epimastigote in the insect vector and the intracellular amastigote in the mammalian host. Also, we identified and characterized a T. cruzi protein (TcHTE) with 55% of sequence similarity to LHR1 (protein involved in L. amazonensis heme transport), which is located in the flagellar pocket, where the transport of nutrients proceeds in trypanosomatids. We postulate TcHTE as a protein involved in improving the efficiency of the heme uptake or trafficking in T. cruzi. |
format |
Article in Journal/Newspaper |
author |
Marcelo L Merli Lucas Pagura Josefina Hernández María Julia Barisón Elizabeth M F Pral Ariel M Silber Julia A Cricco |
author_facet |
Marcelo L Merli Lucas Pagura Josefina Hernández María Julia Barisón Elizabeth M F Pral Ariel M Silber Julia A Cricco |
author_sort |
Marcelo L Merli |
title |
The Trypanosoma cruzi Protein TcHTE Is Critical for Heme Uptake. |
title_short |
The Trypanosoma cruzi Protein TcHTE Is Critical for Heme Uptake. |
title_full |
The Trypanosoma cruzi Protein TcHTE Is Critical for Heme Uptake. |
title_fullStr |
The Trypanosoma cruzi Protein TcHTE Is Critical for Heme Uptake. |
title_full_unstemmed |
The Trypanosoma cruzi Protein TcHTE Is Critical for Heme Uptake. |
title_sort |
trypanosoma cruzi protein tchte is critical for heme uptake. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2016 |
url |
https://doi.org/10.1371/journal.pntd.0004359 https://doaj.org/article/7eab0ba16f8d4e25ba1d751bed3ca43f |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 10, Iss 1, p e0004359 (2016) |
op_relation |
http://europepmc.org/articles/PMC4713871?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004359 https://doaj.org/article/7eab0ba16f8d4e25ba1d751bed3ca43f |
op_doi |
https://doi.org/10.1371/journal.pntd.0004359 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
10 |
container_issue |
1 |
container_start_page |
e0004359 |
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