Microbial Monitoring in the EDEN ISS Greenhouse, a Mobile Test Facility in Antarctica
The EDEN ISS greenhouse, integrated in two joined containers, is a confined mobile test facility in Antarctica for the development and optimization of new plant cultivation techniques for future space programs. The EDEN ISS greenhouse was used successfully from February to November 2018 for fresh fo...
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ftdoajarticles:oai:doaj.org/article:7e8d6499390241c891f14a991399b3d4 2023-05-15T13:36:21+02:00 Microbial Monitoring in the EDEN ISS Greenhouse, a Mobile Test Facility in Antarctica Jana Fahrion Carina Fink Paul Zabel Daniel Schubert Mohamed Mysara Rob Van Houdt Bernhard Eikmanns Kristina Beblo-Vranesevic Petra Rettberg 2020-03-01T00:00:00Z https://doi.org/10.3389/fmicb.2020.00525 https://doaj.org/article/7e8d6499390241c891f14a991399b3d4 EN eng Frontiers Media S.A. https://www.frontiersin.org/article/10.3389/fmicb.2020.00525/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2020.00525 https://doaj.org/article/7e8d6499390241c891f14a991399b3d4 Frontiers in Microbiology, Vol 11 (2020) EDEN ISS greenhouse bacteria plants surfaces phyllosphere Microbiology QR1-502 article 2020 ftdoajarticles https://doi.org/10.3389/fmicb.2020.00525 2022-12-31T09:31:04Z The EDEN ISS greenhouse, integrated in two joined containers, is a confined mobile test facility in Antarctica for the development and optimization of new plant cultivation techniques for future space programs. The EDEN ISS greenhouse was used successfully from February to November 2018 for fresh food production for the overwintering crew at the Antarctic Neumayer III station. During the 9 months of operation, samples from the different plants, from the nutrition solution of the aeroponic planting system, and from diverse surfaces within the three different compartments of the container were taken [future exploration greenhouse (FEG), service section (SS), and cold porch (CP)]. Quantity as well as diversity of microorganisms was examined by cultivation. In case of the plant samples, microbial quantities were in a range from 102 to 104 colony forming units per gram plant material. Compared to plants purchased from a German grocery, the produce hosted orders of magnitude more microorganisms than the EDEN ISS plants. The EDEN ISS plant samples contained mainly fungi and a few bacteria. No classical food associated pathogenic microorganism, like Escherichia and Salmonella, could be found. Probably due to the used cultivation approach, Archaea were not found in the samples. The bioburden in the nutrition solutions increased constantly over time but never reached critical values like 102–103 cfu per 100 mL in irrigation water as it is stated, e.g., for commercial European plant productions. The surface samples revealed high differences in the microbial burden between the greenhouse part of the container and the SS and CP part. However, the numbers of organisms (bacteria and fungi) found in the planted greenhouse were still not critical. The microbial loaded surfaces showed strong temporal as well as spatial fluctuations. In samples of the nutrition solution and the surface, the amount of bacteria exceeded the amount of fungi by many times. For identification, 16S rRNA gene sequencing was performed for the isolated ... Article in Journal/Newspaper Antarc* Antarctic Antarctica Directory of Open Access Journals: DOAJ Articles Antarctic The Antarctic Neumayer Frontiers in Microbiology 11 |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
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topic |
EDEN ISS greenhouse bacteria plants surfaces phyllosphere Microbiology QR1-502 |
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EDEN ISS greenhouse bacteria plants surfaces phyllosphere Microbiology QR1-502 Jana Fahrion Carina Fink Paul Zabel Daniel Schubert Mohamed Mysara Rob Van Houdt Bernhard Eikmanns Kristina Beblo-Vranesevic Petra Rettberg Microbial Monitoring in the EDEN ISS Greenhouse, a Mobile Test Facility in Antarctica |
topic_facet |
EDEN ISS greenhouse bacteria plants surfaces phyllosphere Microbiology QR1-502 |
description |
The EDEN ISS greenhouse, integrated in two joined containers, is a confined mobile test facility in Antarctica for the development and optimization of new plant cultivation techniques for future space programs. The EDEN ISS greenhouse was used successfully from February to November 2018 for fresh food production for the overwintering crew at the Antarctic Neumayer III station. During the 9 months of operation, samples from the different plants, from the nutrition solution of the aeroponic planting system, and from diverse surfaces within the three different compartments of the container were taken [future exploration greenhouse (FEG), service section (SS), and cold porch (CP)]. Quantity as well as diversity of microorganisms was examined by cultivation. In case of the plant samples, microbial quantities were in a range from 102 to 104 colony forming units per gram plant material. Compared to plants purchased from a German grocery, the produce hosted orders of magnitude more microorganisms than the EDEN ISS plants. The EDEN ISS plant samples contained mainly fungi and a few bacteria. No classical food associated pathogenic microorganism, like Escherichia and Salmonella, could be found. Probably due to the used cultivation approach, Archaea were not found in the samples. The bioburden in the nutrition solutions increased constantly over time but never reached critical values like 102–103 cfu per 100 mL in irrigation water as it is stated, e.g., for commercial European plant productions. The surface samples revealed high differences in the microbial burden between the greenhouse part of the container and the SS and CP part. However, the numbers of organisms (bacteria and fungi) found in the planted greenhouse were still not critical. The microbial loaded surfaces showed strong temporal as well as spatial fluctuations. In samples of the nutrition solution and the surface, the amount of bacteria exceeded the amount of fungi by many times. For identification, 16S rRNA gene sequencing was performed for the isolated ... |
format |
Article in Journal/Newspaper |
author |
Jana Fahrion Carina Fink Paul Zabel Daniel Schubert Mohamed Mysara Rob Van Houdt Bernhard Eikmanns Kristina Beblo-Vranesevic Petra Rettberg |
author_facet |
Jana Fahrion Carina Fink Paul Zabel Daniel Schubert Mohamed Mysara Rob Van Houdt Bernhard Eikmanns Kristina Beblo-Vranesevic Petra Rettberg |
author_sort |
Jana Fahrion |
title |
Microbial Monitoring in the EDEN ISS Greenhouse, a Mobile Test Facility in Antarctica |
title_short |
Microbial Monitoring in the EDEN ISS Greenhouse, a Mobile Test Facility in Antarctica |
title_full |
Microbial Monitoring in the EDEN ISS Greenhouse, a Mobile Test Facility in Antarctica |
title_fullStr |
Microbial Monitoring in the EDEN ISS Greenhouse, a Mobile Test Facility in Antarctica |
title_full_unstemmed |
Microbial Monitoring in the EDEN ISS Greenhouse, a Mobile Test Facility in Antarctica |
title_sort |
microbial monitoring in the eden iss greenhouse, a mobile test facility in antarctica |
publisher |
Frontiers Media S.A. |
publishDate |
2020 |
url |
https://doi.org/10.3389/fmicb.2020.00525 https://doaj.org/article/7e8d6499390241c891f14a991399b3d4 |
geographic |
Antarctic The Antarctic Neumayer |
geographic_facet |
Antarctic The Antarctic Neumayer |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_source |
Frontiers in Microbiology, Vol 11 (2020) |
op_relation |
https://www.frontiersin.org/article/10.3389/fmicb.2020.00525/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2020.00525 https://doaj.org/article/7e8d6499390241c891f14a991399b3d4 |
op_doi |
https://doi.org/10.3389/fmicb.2020.00525 |
container_title |
Frontiers in Microbiology |
container_volume |
11 |
_version_ |
1766077564238954496 |