Creating a new biocatalytic complex with extremolipases and biocompatible ionic liquids for improved transesterification reactions
The ongoing energy crisis has spurred increased research into sustainable and more competitive methods for producing biofuels, including biodiesel. In this context, the focus of the current study is to underscore the viability of investing in a novel biocatalytic complex. This complex incorporates e...
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ftdoajarticles:oai:doaj.org/article:7e8732392e2f4d73b60761b6777c972c 2023-11-05T03:36:59+01:00 Creating a new biocatalytic complex with extremolipases and biocompatible ionic liquids for improved transesterification reactions L. González María S. Álvarez Ana Rodríguez María A. Longo Francisco J. Deive 2023-10-01T00:00:00Z https://doi.org/10.1016/j.ecmx.2023.100456 https://doaj.org/article/7e8732392e2f4d73b60761b6777c972c EN eng Elsevier http://www.sciencedirect.com/science/article/pii/S2590174523001125 https://doaj.org/toc/2590-1745 2590-1745 doi:10.1016/j.ecmx.2023.100456 https://doaj.org/article/7e8732392e2f4d73b60761b6777c972c Energy Conversion and Management: X, Vol 20, Iss , Pp 100456- (2023) Transesterification Amino acid and Dipeptide Cholinium Ionic liquids Thermus thermophilus Halomonas Engineering (General). Civil engineering (General) TA1-2040 article 2023 ftdoajarticles https://doi.org/10.1016/j.ecmx.2023.100456 2023-10-08T00:39:37Z The ongoing energy crisis has spurred increased research into sustainable and more competitive methods for producing biofuels, including biodiesel. In this context, the focus of the current study is to underscore the viability of investing in a novel biocatalytic complex. This complex incorporates extremophilic lipases and biocompatible ionic liquids with the aim of achieving exceptionally high conversions in transesterification reactions without generating glycerol. Through a meticulous screening process encompassing various amino acid and dipeptide-based ionic liquids from the ammonium family, cholinium glycinate turned out to be the optimal choice. This selection was driven not only by its enhanced compatibility with a commercially available Candida antarctica lipase B (CaLB) but also with extremophilic enzymes synthesized in-house, derived from halophilic (Halomonas spLM1C) and thermophilic (Thermus thermophilus HB27) strains. Following rigorous testing of both free and immobilized enzymes, the ideal concentration of the ionic liquid in transesterification reactions was determined to be 1% relative to the sunflower oil content. Comparative analysis of conversion rates between immobilized thermophilic lipase and immobilized CaLB revealed the efficacy of the proposed approach. Maximum conversions were found to increase by 20%, with specific conversion rates soaring by approximately 180% when utilizing the immobilized thermophilic lipase. In conclusion, this research ushers in new prospects for advancing the competitiveness of biocatalytic solutions in glycerol-free transesterification reactions, underscoring its potential to revolutionize the landscape of sustainable energy production. Article in Journal/Newspaper Antarc* Antarctica Directory of Open Access Journals: DOAJ Articles Energy Conversion and Management: X 20 100456 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Transesterification Amino acid and Dipeptide Cholinium Ionic liquids Thermus thermophilus Halomonas Engineering (General). Civil engineering (General) TA1-2040 |
spellingShingle |
Transesterification Amino acid and Dipeptide Cholinium Ionic liquids Thermus thermophilus Halomonas Engineering (General). Civil engineering (General) TA1-2040 L. González María S. Álvarez Ana Rodríguez María A. Longo Francisco J. Deive Creating a new biocatalytic complex with extremolipases and biocompatible ionic liquids for improved transesterification reactions |
topic_facet |
Transesterification Amino acid and Dipeptide Cholinium Ionic liquids Thermus thermophilus Halomonas Engineering (General). Civil engineering (General) TA1-2040 |
description |
The ongoing energy crisis has spurred increased research into sustainable and more competitive methods for producing biofuels, including biodiesel. In this context, the focus of the current study is to underscore the viability of investing in a novel biocatalytic complex. This complex incorporates extremophilic lipases and biocompatible ionic liquids with the aim of achieving exceptionally high conversions in transesterification reactions without generating glycerol. Through a meticulous screening process encompassing various amino acid and dipeptide-based ionic liquids from the ammonium family, cholinium glycinate turned out to be the optimal choice. This selection was driven not only by its enhanced compatibility with a commercially available Candida antarctica lipase B (CaLB) but also with extremophilic enzymes synthesized in-house, derived from halophilic (Halomonas spLM1C) and thermophilic (Thermus thermophilus HB27) strains. Following rigorous testing of both free and immobilized enzymes, the ideal concentration of the ionic liquid in transesterification reactions was determined to be 1% relative to the sunflower oil content. Comparative analysis of conversion rates between immobilized thermophilic lipase and immobilized CaLB revealed the efficacy of the proposed approach. Maximum conversions were found to increase by 20%, with specific conversion rates soaring by approximately 180% when utilizing the immobilized thermophilic lipase. In conclusion, this research ushers in new prospects for advancing the competitiveness of biocatalytic solutions in glycerol-free transesterification reactions, underscoring its potential to revolutionize the landscape of sustainable energy production. |
format |
Article in Journal/Newspaper |
author |
L. González María S. Álvarez Ana Rodríguez María A. Longo Francisco J. Deive |
author_facet |
L. González María S. Álvarez Ana Rodríguez María A. Longo Francisco J. Deive |
author_sort |
L. González |
title |
Creating a new biocatalytic complex with extremolipases and biocompatible ionic liquids for improved transesterification reactions |
title_short |
Creating a new biocatalytic complex with extremolipases and biocompatible ionic liquids for improved transesterification reactions |
title_full |
Creating a new biocatalytic complex with extremolipases and biocompatible ionic liquids for improved transesterification reactions |
title_fullStr |
Creating a new biocatalytic complex with extremolipases and biocompatible ionic liquids for improved transesterification reactions |
title_full_unstemmed |
Creating a new biocatalytic complex with extremolipases and biocompatible ionic liquids for improved transesterification reactions |
title_sort |
creating a new biocatalytic complex with extremolipases and biocompatible ionic liquids for improved transesterification reactions |
publisher |
Elsevier |
publishDate |
2023 |
url |
https://doi.org/10.1016/j.ecmx.2023.100456 https://doaj.org/article/7e8732392e2f4d73b60761b6777c972c |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Energy Conversion and Management: X, Vol 20, Iss , Pp 100456- (2023) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2590174523001125 https://doaj.org/toc/2590-1745 2590-1745 doi:10.1016/j.ecmx.2023.100456 https://doaj.org/article/7e8732392e2f4d73b60761b6777c972c |
op_doi |
https://doi.org/10.1016/j.ecmx.2023.100456 |
container_title |
Energy Conversion and Management: X |
container_volume |
20 |
container_start_page |
100456 |
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1781692347433615360 |