Antigestagens Mediate the Expression of Decidualization Markers, Extracellular Matrix Factors and Connexin 43 in Decidualized Dog Uterine Stromal (DUS) Cells
Feto-maternal communication in the dog involves the differentiation of stromal cells into decidual cells. As the only placental cells expressing the nuclear progesterone (P4) receptor (PGR), decidual cells play crucial roles in the maintenance and termination of pregnancy. Accordingly, to investigat...
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MDPI AG
2022
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Online Access: | https://doi.org/10.3390/ani12070798 https://doaj.org/article/7e59fa0b74dd43c8a8e87f93a0c895fe |
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ftdoajarticles:oai:doaj.org/article:7e59fa0b74dd43c8a8e87f93a0c895fe 2023-05-15T15:50:45+02:00 Antigestagens Mediate the Expression of Decidualization Markers, Extracellular Matrix Factors and Connexin 43 in Decidualized Dog Uterine Stromal (DUS) Cells Ali Kazemian Miguel Tavares Pereira Bernd Hoffmann Mariusz P. Kowalewski 2022-03-01T00:00:00Z https://doi.org/10.3390/ani12070798 https://doaj.org/article/7e59fa0b74dd43c8a8e87f93a0c895fe EN eng MDPI AG https://www.mdpi.com/2076-2615/12/7/798 https://doaj.org/toc/2076-2615 doi:10.3390/ani12070798 2076-2615 https://doaj.org/article/7e59fa0b74dd43c8a8e87f93a0c895fe Animals, Vol 12, Iss 798, p 798 (2022) dog ( Canis lupus familiaris ) dog uterine stromal (DUS) cells decidualization nuclear progesterone receptor (PGR) antigestagens Veterinary medicine SF600-1100 Zoology QL1-991 article 2022 ftdoajarticles https://doi.org/10.3390/ani12070798 2022-12-30T22:21:36Z Feto-maternal communication in the dog involves the differentiation of stromal cells into decidual cells. As the only placental cells expressing the nuclear progesterone (P4) receptor (PGR), decidual cells play crucial roles in the maintenance and termination of pregnancy. Accordingly, to investigate possible PGR-mediated mechanisms in canine decidual cells, in vitro decidualized dog uterine stromal (DUS) cells were treated with functional PGR-blockers, mifepristone and aglepristone. Effects on decidualization markers, epithelial and mesenchymal factors, and markers of cellular viability were assessed. Decidualization increased the expression of PTGES , PGR , IGF1 , and PRLR, along with ECM1 , COL4 and CX43, but downregulated IGF2 . DUS cells retained their mesenchymal character, and the expression of COL4 indicated the mesenchymal-epithelial transformation. Antigestagen treatment decreased the availability of PTGES , PRLR , IGF1 and PGR. Furthermore, antigestagens decreased the mRNA and protein expression of CX43, and transcriptional levels of ECM1 and COL4 . Additionally, antigestagens increased levels of activated-CASP3 (a proapoptotic factor), associated with lowered levels of PCNA (a proliferation marker). These data reveal important aspects of the functional involvement of PGR in canine decidual cells, regarding the expression of decidualization markers and acquisition of epithelial-like characteristics. Some of these mechanisms may be crucial for the maintenance and/or termination of canine pregnancy. Article in Journal/Newspaper Canis lupus Directory of Open Access Journals: DOAJ Articles Animals 12 7 798 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
dog ( Canis lupus familiaris ) dog uterine stromal (DUS) cells decidualization nuclear progesterone receptor (PGR) antigestagens Veterinary medicine SF600-1100 Zoology QL1-991 |
spellingShingle |
dog ( Canis lupus familiaris ) dog uterine stromal (DUS) cells decidualization nuclear progesterone receptor (PGR) antigestagens Veterinary medicine SF600-1100 Zoology QL1-991 Ali Kazemian Miguel Tavares Pereira Bernd Hoffmann Mariusz P. Kowalewski Antigestagens Mediate the Expression of Decidualization Markers, Extracellular Matrix Factors and Connexin 43 in Decidualized Dog Uterine Stromal (DUS) Cells |
topic_facet |
dog ( Canis lupus familiaris ) dog uterine stromal (DUS) cells decidualization nuclear progesterone receptor (PGR) antigestagens Veterinary medicine SF600-1100 Zoology QL1-991 |
description |
Feto-maternal communication in the dog involves the differentiation of stromal cells into decidual cells. As the only placental cells expressing the nuclear progesterone (P4) receptor (PGR), decidual cells play crucial roles in the maintenance and termination of pregnancy. Accordingly, to investigate possible PGR-mediated mechanisms in canine decidual cells, in vitro decidualized dog uterine stromal (DUS) cells were treated with functional PGR-blockers, mifepristone and aglepristone. Effects on decidualization markers, epithelial and mesenchymal factors, and markers of cellular viability were assessed. Decidualization increased the expression of PTGES , PGR , IGF1 , and PRLR, along with ECM1 , COL4 and CX43, but downregulated IGF2 . DUS cells retained their mesenchymal character, and the expression of COL4 indicated the mesenchymal-epithelial transformation. Antigestagen treatment decreased the availability of PTGES , PRLR , IGF1 and PGR. Furthermore, antigestagens decreased the mRNA and protein expression of CX43, and transcriptional levels of ECM1 and COL4 . Additionally, antigestagens increased levels of activated-CASP3 (a proapoptotic factor), associated with lowered levels of PCNA (a proliferation marker). These data reveal important aspects of the functional involvement of PGR in canine decidual cells, regarding the expression of decidualization markers and acquisition of epithelial-like characteristics. Some of these mechanisms may be crucial for the maintenance and/or termination of canine pregnancy. |
format |
Article in Journal/Newspaper |
author |
Ali Kazemian Miguel Tavares Pereira Bernd Hoffmann Mariusz P. Kowalewski |
author_facet |
Ali Kazemian Miguel Tavares Pereira Bernd Hoffmann Mariusz P. Kowalewski |
author_sort |
Ali Kazemian |
title |
Antigestagens Mediate the Expression of Decidualization Markers, Extracellular Matrix Factors and Connexin 43 in Decidualized Dog Uterine Stromal (DUS) Cells |
title_short |
Antigestagens Mediate the Expression of Decidualization Markers, Extracellular Matrix Factors and Connexin 43 in Decidualized Dog Uterine Stromal (DUS) Cells |
title_full |
Antigestagens Mediate the Expression of Decidualization Markers, Extracellular Matrix Factors and Connexin 43 in Decidualized Dog Uterine Stromal (DUS) Cells |
title_fullStr |
Antigestagens Mediate the Expression of Decidualization Markers, Extracellular Matrix Factors and Connexin 43 in Decidualized Dog Uterine Stromal (DUS) Cells |
title_full_unstemmed |
Antigestagens Mediate the Expression of Decidualization Markers, Extracellular Matrix Factors and Connexin 43 in Decidualized Dog Uterine Stromal (DUS) Cells |
title_sort |
antigestagens mediate the expression of decidualization markers, extracellular matrix factors and connexin 43 in decidualized dog uterine stromal (dus) cells |
publisher |
MDPI AG |
publishDate |
2022 |
url |
https://doi.org/10.3390/ani12070798 https://doaj.org/article/7e59fa0b74dd43c8a8e87f93a0c895fe |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_source |
Animals, Vol 12, Iss 798, p 798 (2022) |
op_relation |
https://www.mdpi.com/2076-2615/12/7/798 https://doaj.org/toc/2076-2615 doi:10.3390/ani12070798 2076-2615 https://doaj.org/article/7e59fa0b74dd43c8a8e87f93a0c895fe |
op_doi |
https://doi.org/10.3390/ani12070798 |
container_title |
Animals |
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12 |
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7 |
container_start_page |
798 |
_version_ |
1766385771018715136 |