Construction and use of Plasmodium falciparum phage display libraries to identify host parasite interactions
Abstract Background The development of Plasmodium falciparum within human erythrocytes induces a wide array of changes in the ultrastructure, function and antigenic properties of the host cell. Numerous proteins encoded by the parasite have been shown to interact with the erythrocyte membrane. The i...
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ftdoajarticles:oai:doaj.org/article:7e58b98249104b20a813e2fab6f01f68 2023-05-15T15:07:36+02:00 Construction and use of Plasmodium falciparum phage display libraries to identify host parasite interactions Coetzer Theresa L Lanzillotti Roberto Lauterbach Sonja B 2003-12-01T00:00:00Z https://doi.org/10.1186/1475-2875-2-47 https://doaj.org/article/7e58b98249104b20a813e2fab6f01f68 EN eng BMC http://www.malariajournal.com/content/2/1/47 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-2-47 1475-2875 https://doaj.org/article/7e58b98249104b20a813e2fab6f01f68 Malaria Journal, Vol 2, Iss 1, p 47 (2003) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2003 ftdoajarticles https://doi.org/10.1186/1475-2875-2-47 2022-12-31T08:10:58Z Abstract Background The development of Plasmodium falciparum within human erythrocytes induces a wide array of changes in the ultrastructure, function and antigenic properties of the host cell. Numerous proteins encoded by the parasite have been shown to interact with the erythrocyte membrane. The identification of new interactions between human erythrocyte and P. falciparum proteins has formed a key area of malaria research. To circumvent the difficulties provided by conventional protein techniques, a novel application of the phage display technology was utilised. Methods P. falciparum phage display libraries were created and biopanned against purified erythrocyte membrane proteins. The identification of interacting and in-frame amino acid sequences was achieved by sequencing parasite cDNA inserts and performing bioinformatic analyses in the PlasmoDB database. Results Following four rounds of biopanning, sequencing and bioinformatic investigations, seven P. falciparum proteins with significant binding specificity toward human erythrocyte spectrin and protein 4.1 were identified. The specificity of these P. falciparum proteins were demonstrated by the marked enrichment of the respective in-frame binding sequences from a fourth round phage display library. Conclusion The construction and biopanning of P. falciparum phage display expression libraries provide a novel approach for the identification of new interactions between the parasite and the erythrocyte membrane. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 2 1 47 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Coetzer Theresa L Lanzillotti Roberto Lauterbach Sonja B Construction and use of Plasmodium falciparum phage display libraries to identify host parasite interactions |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background The development of Plasmodium falciparum within human erythrocytes induces a wide array of changes in the ultrastructure, function and antigenic properties of the host cell. Numerous proteins encoded by the parasite have been shown to interact with the erythrocyte membrane. The identification of new interactions between human erythrocyte and P. falciparum proteins has formed a key area of malaria research. To circumvent the difficulties provided by conventional protein techniques, a novel application of the phage display technology was utilised. Methods P. falciparum phage display libraries were created and biopanned against purified erythrocyte membrane proteins. The identification of interacting and in-frame amino acid sequences was achieved by sequencing parasite cDNA inserts and performing bioinformatic analyses in the PlasmoDB database. Results Following four rounds of biopanning, sequencing and bioinformatic investigations, seven P. falciparum proteins with significant binding specificity toward human erythrocyte spectrin and protein 4.1 were identified. The specificity of these P. falciparum proteins were demonstrated by the marked enrichment of the respective in-frame binding sequences from a fourth round phage display library. Conclusion The construction and biopanning of P. falciparum phage display expression libraries provide a novel approach for the identification of new interactions between the parasite and the erythrocyte membrane. |
format |
Article in Journal/Newspaper |
author |
Coetzer Theresa L Lanzillotti Roberto Lauterbach Sonja B |
author_facet |
Coetzer Theresa L Lanzillotti Roberto Lauterbach Sonja B |
author_sort |
Coetzer Theresa L |
title |
Construction and use of Plasmodium falciparum phage display libraries to identify host parasite interactions |
title_short |
Construction and use of Plasmodium falciparum phage display libraries to identify host parasite interactions |
title_full |
Construction and use of Plasmodium falciparum phage display libraries to identify host parasite interactions |
title_fullStr |
Construction and use of Plasmodium falciparum phage display libraries to identify host parasite interactions |
title_full_unstemmed |
Construction and use of Plasmodium falciparum phage display libraries to identify host parasite interactions |
title_sort |
construction and use of plasmodium falciparum phage display libraries to identify host parasite interactions |
publisher |
BMC |
publishDate |
2003 |
url |
https://doi.org/10.1186/1475-2875-2-47 https://doaj.org/article/7e58b98249104b20a813e2fab6f01f68 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 2, Iss 1, p 47 (2003) |
op_relation |
http://www.malariajournal.com/content/2/1/47 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-2-47 1475-2875 https://doaj.org/article/7e58b98249104b20a813e2fab6f01f68 |
op_doi |
https://doi.org/10.1186/1475-2875-2-47 |
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Malaria Journal |
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2 |
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1 |
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47 |
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1766339077232132096 |