Antimicrobial, cytotoxicity, anticancer and antioxidant activities of Jatropha zeyheri Sond. roots (Euphorbiaceae)
Objective: To evaluate the antimicrobial, antioxidant, cytotoxicity and anticancer activity of fractions from Jatropha zeyheri roots and to explore the phytochemical profile of the most biologically active fraction. Methods: Fractions from Jatropha zeyheri ethyl acetate extract were investigated for...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
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Wolters Kluwer Medknow Publications
2019
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Online Access: | https://doi.org/10.4103/2221-1691.261822 https://doaj.org/article/7e3622ed122d4157a81e7f19fa7feee9 |
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ftdoajarticles:oai:doaj.org/article:7e3622ed122d4157a81e7f19fa7feee9 2023-05-15T15:16:03+02:00 Antimicrobial, cytotoxicity, anticancer and antioxidant activities of Jatropha zeyheri Sond. roots (Euphorbiaceae) N I Mongalo O S Soyingbe T J Makhafola 2019-01-01T00:00:00Z https://doi.org/10.4103/2221-1691.261822 https://doaj.org/article/7e3622ed122d4157a81e7f19fa7feee9 EN eng Wolters Kluwer Medknow Publications http://www.apjtb.org/article.asp?issn=2221-1691;year=2019;volume=9;issue=7;spage=307;epage=314;aulast=Mongalo https://doaj.org/toc/2221-1691 2221-1691 doi:10.4103/2221-1691.261822 https://doaj.org/article/7e3622ed122d4157a81e7f19fa7feee9 Asian Pacific Journal of Tropical Biomedicine, Vol 9, Iss 7, Pp 307-314 (2019) antimicrobial activity selectivity index jatropha zeyheri gc-ms antioxidant activity anti-proliferative effect Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2019 ftdoajarticles https://doi.org/10.4103/2221-1691.261822 2022-12-31T02:10:54Z Objective: To evaluate the antimicrobial, antioxidant, cytotoxicity and anticancer activity of fractions from Jatropha zeyheri roots and to explore the phytochemical profile of the most biologically active fraction. Methods: Fractions from Jatropha zeyheri ethyl acetate extract were investigated for antimicrobial activity against a plethora of pathogenic microorganisms of different origins. The cytotoxicity studies of fractions were evaluated in vitro using tetrazolium-based calorimetric assay against human dermal fibroblast, colon adenocarcinoma (Caco-2), breast cancer (MCF-7) and lung cancer (A547) cell lines. The anti-oxidant activity of fractions was determined in vitro against 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2’-azino-bis-(3-ethylbenzothiazoline- 6-sulfonic acid) diammonium salt (ABTS) and chelation of iron (Fe2+). Gas chromatography mass spectrometry analysis was performed to detect phytochemical constituents in fraction with potent biological activity. Results: Fraction 2 of Jatropha zeyheri roots exhibited the lowest minimum inhibitory concentration of 40 μg/mL against Klebsiella pneumoniae and 80 μg/mL against Candida albicans, Staphylococcus aureus and Mycoplasma hominis. The fractions revealed some varying degrees of cytotoxicity against human dermal fibroblasts yielding LC50 values ranging from 28.96 to 166.52 μg/mL. Fraction 3 exhibited the highest selectivity index value of 2.08 against Klebsiella pneumoniae. Moreover, fraction 2 selectively inhibited the growth of Caco-2 with LC50 of 8.83 μg/mL, compared to other cancerous cell lines. Fraction 2 of Jatropha zeyheri further exhibited IC50 of 19.66, 22.63 and 1.82 μg/mL against DPPH, ABTS and Fe2+, respectively. Gas chromatography mass spectrometry analysis revealed the presence of cyclotetracosane (10.08%), 9-hexacosene (9.40%), hexadecanoic acid (3.90%), (Z)-9-octadecenamide (3.63%), octacosane (2.27%), 11-n-decylheneicosane (2.23%), ethyl vallesiachotamate (2.17%), heneicosanoic acid (2.10%), and octadecanoic acid (2.08%) in fraction 2 of ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 9 7 307 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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antimicrobial activity selectivity index jatropha zeyheri gc-ms antioxidant activity anti-proliferative effect Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
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antimicrobial activity selectivity index jatropha zeyheri gc-ms antioxidant activity anti-proliferative effect Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 N I Mongalo O S Soyingbe T J Makhafola Antimicrobial, cytotoxicity, anticancer and antioxidant activities of Jatropha zeyheri Sond. roots (Euphorbiaceae) |
topic_facet |
antimicrobial activity selectivity index jatropha zeyheri gc-ms antioxidant activity anti-proliferative effect Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To evaluate the antimicrobial, antioxidant, cytotoxicity and anticancer activity of fractions from Jatropha zeyheri roots and to explore the phytochemical profile of the most biologically active fraction. Methods: Fractions from Jatropha zeyheri ethyl acetate extract were investigated for antimicrobial activity against a plethora of pathogenic microorganisms of different origins. The cytotoxicity studies of fractions were evaluated in vitro using tetrazolium-based calorimetric assay against human dermal fibroblast, colon adenocarcinoma (Caco-2), breast cancer (MCF-7) and lung cancer (A547) cell lines. The anti-oxidant activity of fractions was determined in vitro against 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2’-azino-bis-(3-ethylbenzothiazoline- 6-sulfonic acid) diammonium salt (ABTS) and chelation of iron (Fe2+). Gas chromatography mass spectrometry analysis was performed to detect phytochemical constituents in fraction with potent biological activity. Results: Fraction 2 of Jatropha zeyheri roots exhibited the lowest minimum inhibitory concentration of 40 μg/mL against Klebsiella pneumoniae and 80 μg/mL against Candida albicans, Staphylococcus aureus and Mycoplasma hominis. The fractions revealed some varying degrees of cytotoxicity against human dermal fibroblasts yielding LC50 values ranging from 28.96 to 166.52 μg/mL. Fraction 3 exhibited the highest selectivity index value of 2.08 against Klebsiella pneumoniae. Moreover, fraction 2 selectively inhibited the growth of Caco-2 with LC50 of 8.83 μg/mL, compared to other cancerous cell lines. Fraction 2 of Jatropha zeyheri further exhibited IC50 of 19.66, 22.63 and 1.82 μg/mL against DPPH, ABTS and Fe2+, respectively. Gas chromatography mass spectrometry analysis revealed the presence of cyclotetracosane (10.08%), 9-hexacosene (9.40%), hexadecanoic acid (3.90%), (Z)-9-octadecenamide (3.63%), octacosane (2.27%), 11-n-decylheneicosane (2.23%), ethyl vallesiachotamate (2.17%), heneicosanoic acid (2.10%), and octadecanoic acid (2.08%) in fraction 2 of ... |
format |
Article in Journal/Newspaper |
author |
N I Mongalo O S Soyingbe T J Makhafola |
author_facet |
N I Mongalo O S Soyingbe T J Makhafola |
author_sort |
N I Mongalo |
title |
Antimicrobial, cytotoxicity, anticancer and antioxidant activities of Jatropha zeyheri Sond. roots (Euphorbiaceae) |
title_short |
Antimicrobial, cytotoxicity, anticancer and antioxidant activities of Jatropha zeyheri Sond. roots (Euphorbiaceae) |
title_full |
Antimicrobial, cytotoxicity, anticancer and antioxidant activities of Jatropha zeyheri Sond. roots (Euphorbiaceae) |
title_fullStr |
Antimicrobial, cytotoxicity, anticancer and antioxidant activities of Jatropha zeyheri Sond. roots (Euphorbiaceae) |
title_full_unstemmed |
Antimicrobial, cytotoxicity, anticancer and antioxidant activities of Jatropha zeyheri Sond. roots (Euphorbiaceae) |
title_sort |
antimicrobial, cytotoxicity, anticancer and antioxidant activities of jatropha zeyheri sond. roots (euphorbiaceae) |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2019 |
url |
https://doi.org/10.4103/2221-1691.261822 https://doaj.org/article/7e3622ed122d4157a81e7f19fa7feee9 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 9, Iss 7, Pp 307-314 (2019) |
op_relation |
http://www.apjtb.org/article.asp?issn=2221-1691;year=2019;volume=9;issue=7;spage=307;epage=314;aulast=Mongalo https://doaj.org/toc/2221-1691 2221-1691 doi:10.4103/2221-1691.261822 https://doaj.org/article/7e3622ed122d4157a81e7f19fa7feee9 |
op_doi |
https://doi.org/10.4103/2221-1691.261822 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
9 |
container_issue |
7 |
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307 |
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1766346367646564352 |