Surveillance of pfhrp2 and pfhrp3 gene deletions among symptomatic Plasmodium falciparum malaria patients in Central Vietnam

Abstract Background Malaria rapid diagnostic tests (RDTs) remain the main point-of-care tests for diagnosis of symptomatic Plasmodium falciparum malaria in endemic areas. However, parasites with gene deletions in the most common RDT target, histidine rich protein 2 (pfhrp2/HRP2), can produce false-n...

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Published in:Malaria Journal
Main Authors: Ngo Duc Thang, Eduard Rovira-Vallbona, Nguyen Thi Huong Binh, Dang Viet Dung, Nguyen Thi Hong Ngoc, Tran Khanh Long, Tran Thanh Duong, Nicholas J. Martin, Kimberly A. Edgel
Format: Article in Journal/Newspaper
Language:English
Published: BMC 2022
Subjects:
Online Access:https://doi.org/10.1186/s12936-022-04399-w
https://doaj.org/article/7db9907ad6c2436095410a4ba860ec0d
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spelling ftdoajarticles:oai:doaj.org/article:7db9907ad6c2436095410a4ba860ec0d 2023-05-15T15:10:23+02:00 Surveillance of pfhrp2 and pfhrp3 gene deletions among symptomatic Plasmodium falciparum malaria patients in Central Vietnam Ngo Duc Thang Eduard Rovira-Vallbona Nguyen Thi Huong Binh Dang Viet Dung Nguyen Thi Hong Ngoc Tran Khanh Long Tran Thanh Duong Nicholas J. Martin Kimberly A. Edgel 2022-12-01T00:00:00Z https://doi.org/10.1186/s12936-022-04399-w https://doaj.org/article/7db9907ad6c2436095410a4ba860ec0d EN eng BMC https://doi.org/10.1186/s12936-022-04399-w https://doaj.org/toc/1475-2875 doi:10.1186/s12936-022-04399-w 1475-2875 https://doaj.org/article/7db9907ad6c2436095410a4ba860ec0d Malaria Journal, Vol 21, Iss 1, Pp 1-8 (2022) Malaria Plasmodium falciparum Diagnostics Rapid Diagnostic Test Histidine rich protein 2 Histidine rich protein 3 Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2022 ftdoajarticles https://doi.org/10.1186/s12936-022-04399-w 2022-12-30T21:01:22Z Abstract Background Malaria rapid diagnostic tests (RDTs) remain the main point-of-care tests for diagnosis of symptomatic Plasmodium falciparum malaria in endemic areas. However, parasites with gene deletions in the most common RDT target, histidine rich protein 2 (pfhrp2/HRP2), can produce false-negative RDT results leading to inadequate case management. The objective of this study was to determine the prevalence of hrp2/3 deletions causing false-negative RDT results in Vietnam (Gia Lai and Dak Lak provinces). Methods Individuals presenting with malaria symptoms at health facilities were screened for P. falciparum infection using light microscopy and HRP2-RDT (SD Bioline Malaria Antigen Pf/Pv RDT, Abbott). Microscopically confirmed P. falciparum infections were analysed for parasite species by 18S rRNA qPCR, and pfhrp2 and pfhrp3 exon2 deletions were investigated by nested PCR. pfhrp2 amplicons were sequenced by the Sanger method and HRP2 plasma levels were determined by enzyme-linked immunosorbent assay (ELISA). Results The prevalence of false-negative RDT results among symptomatic cases was 5.6% (15/270). No pfhrp2 and pfhrp3 deletions were identified. False-negative RDT results were associated with lower parasite density (p = 0.005) and lower HRP2 plasma concentrations (p < 0.001), as compared to positive RDT. Conclusions The absence of hrp2/3 deletions detected in this survey suggests that HRP2-based malaria RDTs remain effective for the diagnosis of symptomatic P. falciparum malaria in Central Vietnam. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Abbott ENVELOPE(-62.133,-62.133,-64.100,-64.100) Malaria Journal 21 1
institution Open Polar
collection Directory of Open Access Journals: DOAJ Articles
op_collection_id ftdoajarticles
language English
topic Malaria
Plasmodium falciparum
Diagnostics
Rapid Diagnostic Test
Histidine rich protein 2
Histidine rich protein 3
Arctic medicine. Tropical medicine
RC955-962
Infectious and parasitic diseases
RC109-216
spellingShingle Malaria
Plasmodium falciparum
Diagnostics
Rapid Diagnostic Test
Histidine rich protein 2
Histidine rich protein 3
Arctic medicine. Tropical medicine
RC955-962
Infectious and parasitic diseases
RC109-216
Ngo Duc Thang
Eduard Rovira-Vallbona
Nguyen Thi Huong Binh
Dang Viet Dung
Nguyen Thi Hong Ngoc
Tran Khanh Long
Tran Thanh Duong
Nicholas J. Martin
Kimberly A. Edgel
Surveillance of pfhrp2 and pfhrp3 gene deletions among symptomatic Plasmodium falciparum malaria patients in Central Vietnam
topic_facet Malaria
Plasmodium falciparum
Diagnostics
Rapid Diagnostic Test
Histidine rich protein 2
Histidine rich protein 3
Arctic medicine. Tropical medicine
RC955-962
Infectious and parasitic diseases
RC109-216
description Abstract Background Malaria rapid diagnostic tests (RDTs) remain the main point-of-care tests for diagnosis of symptomatic Plasmodium falciparum malaria in endemic areas. However, parasites with gene deletions in the most common RDT target, histidine rich protein 2 (pfhrp2/HRP2), can produce false-negative RDT results leading to inadequate case management. The objective of this study was to determine the prevalence of hrp2/3 deletions causing false-negative RDT results in Vietnam (Gia Lai and Dak Lak provinces). Methods Individuals presenting with malaria symptoms at health facilities were screened for P. falciparum infection using light microscopy and HRP2-RDT (SD Bioline Malaria Antigen Pf/Pv RDT, Abbott). Microscopically confirmed P. falciparum infections were analysed for parasite species by 18S rRNA qPCR, and pfhrp2 and pfhrp3 exon2 deletions were investigated by nested PCR. pfhrp2 amplicons were sequenced by the Sanger method and HRP2 plasma levels were determined by enzyme-linked immunosorbent assay (ELISA). Results The prevalence of false-negative RDT results among symptomatic cases was 5.6% (15/270). No pfhrp2 and pfhrp3 deletions were identified. False-negative RDT results were associated with lower parasite density (p = 0.005) and lower HRP2 plasma concentrations (p < 0.001), as compared to positive RDT. Conclusions The absence of hrp2/3 deletions detected in this survey suggests that HRP2-based malaria RDTs remain effective for the diagnosis of symptomatic P. falciparum malaria in Central Vietnam.
format Article in Journal/Newspaper
author Ngo Duc Thang
Eduard Rovira-Vallbona
Nguyen Thi Huong Binh
Dang Viet Dung
Nguyen Thi Hong Ngoc
Tran Khanh Long
Tran Thanh Duong
Nicholas J. Martin
Kimberly A. Edgel
author_facet Ngo Duc Thang
Eduard Rovira-Vallbona
Nguyen Thi Huong Binh
Dang Viet Dung
Nguyen Thi Hong Ngoc
Tran Khanh Long
Tran Thanh Duong
Nicholas J. Martin
Kimberly A. Edgel
author_sort Ngo Duc Thang
title Surveillance of pfhrp2 and pfhrp3 gene deletions among symptomatic Plasmodium falciparum malaria patients in Central Vietnam
title_short Surveillance of pfhrp2 and pfhrp3 gene deletions among symptomatic Plasmodium falciparum malaria patients in Central Vietnam
title_full Surveillance of pfhrp2 and pfhrp3 gene deletions among symptomatic Plasmodium falciparum malaria patients in Central Vietnam
title_fullStr Surveillance of pfhrp2 and pfhrp3 gene deletions among symptomatic Plasmodium falciparum malaria patients in Central Vietnam
title_full_unstemmed Surveillance of pfhrp2 and pfhrp3 gene deletions among symptomatic Plasmodium falciparum malaria patients in Central Vietnam
title_sort surveillance of pfhrp2 and pfhrp3 gene deletions among symptomatic plasmodium falciparum malaria patients in central vietnam
publisher BMC
publishDate 2022
url https://doi.org/10.1186/s12936-022-04399-w
https://doaj.org/article/7db9907ad6c2436095410a4ba860ec0d
long_lat ENVELOPE(-62.133,-62.133,-64.100,-64.100)
geographic Arctic
Abbott
geographic_facet Arctic
Abbott
genre Arctic
genre_facet Arctic
op_source Malaria Journal, Vol 21, Iss 1, Pp 1-8 (2022)
op_relation https://doi.org/10.1186/s12936-022-04399-w
https://doaj.org/toc/1475-2875
doi:10.1186/s12936-022-04399-w
1475-2875
https://doaj.org/article/7db9907ad6c2436095410a4ba860ec0d
op_doi https://doi.org/10.1186/s12936-022-04399-w
container_title Malaria Journal
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