Chondroitin Sulfate and Fucosylated Chondroitin Sulfate as Stimulators of Hematopoiesis in Cyclophosphamide-Induced Mice
The immunosuppression and inhibition of hematopoiesis are considered to be reasons for the development of complications after intensive chemotherapy and allogeneic hematopoietic stem cell transplantation. Chondroitin sulfate ( CS ), isolated from the fish Salmo salar , and fucosylated chondroitin su...
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2021
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ftdoajarticles:oai:doaj.org/article:7d890cf1cf0d4914a77618a120abbfa9 2023-05-15T18:09:57+02:00 Chondroitin Sulfate and Fucosylated Chondroitin Sulfate as Stimulators of Hematopoiesis in Cyclophosphamide-Induced Mice Nadezhda E. Ustyuzhanina Natalia Yu. Anisimova Maria I. Bilan Fedor V. Donenko Galina E. Morozevich Dmitriy V. Yashunskiy Anatolii I. Usov Nara G. Siminyan Kirill I. Kirgisov Svetlana R. Varfolomeeva Mikhail V. Kiselevskiy Nikolay E. Nifantiev 2021-10-01T00:00:00Z https://doi.org/10.3390/ph14111074 https://doaj.org/article/7d890cf1cf0d4914a77618a120abbfa9 EN eng MDPI AG https://www.mdpi.com/1424-8247/14/11/1074 https://doaj.org/toc/1424-8247 doi:10.3390/ph14111074 1424-8247 https://doaj.org/article/7d890cf1cf0d4914a77618a120abbfa9 Pharmaceuticals, Vol 14, Iss 1074, p 1074 (2021) graft versus host disease granulocyte colony-stimulating factor chondroitin sulfate fucosylated chondroitin sulfate hematopoiesis immunosuppression Medicine R Pharmacy and materia medica RS1-441 article 2021 ftdoajarticles https://doi.org/10.3390/ph14111074 2022-12-31T05:20:58Z The immunosuppression and inhibition of hematopoiesis are considered to be reasons for the development of complications after intensive chemotherapy and allogeneic hematopoietic stem cell transplantation. Chondroitin sulfate ( CS ), isolated from the fish Salmo salar , and fucosylated chondroitin sulfate ( FCS ), isolated from the sea cucumber Apostichopus japonicus , were studied for their roles as stimulators of hematopoiesis in a model of cyclophosphamide-induced immunosuppression in mice. The recombinant protein r G-CSF was applied as a reference. The studied polysaccharides were shown to stimulate the release of white and red blood cells, as well as platelets from bone marrow in immunosuppressed mice, while r G-CSF was only responsible for the significant increase in the level of leucocytes. The analysis of different populations of leucocytes in blood indicated that r G-CSF mainly stimulated the production of neutrophils, whereas in the cases of the studied saccharides, increases in the levels of monocytes, lymphocytes and neutrophils were observed. The normalization of the level of the pro-inflammatory cytokine IL-6 in the serum and the recovery of cell populations in the spleen were observed in immunosuppressed mice following treatment with the polysaccharides. An increase in the proliferative activity of hematopoietic cells CD34(+)CD45(+) was observed following ex vivo polysaccharide exposure. Further study on related oligosaccharides regarding their potential as promising drugs in the complex prophylaxis and therapy of hematopoiesis inhibition after intensive chemotherapy and allogeneic hematopoietic stem cell transplantation seems to be warranted. Article in Journal/Newspaper Salmo salar Directory of Open Access Journals: DOAJ Articles Pharmaceuticals 14 11 1074 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
graft versus host disease granulocyte colony-stimulating factor chondroitin sulfate fucosylated chondroitin sulfate hematopoiesis immunosuppression Medicine R Pharmacy and materia medica RS1-441 |
spellingShingle |
graft versus host disease granulocyte colony-stimulating factor chondroitin sulfate fucosylated chondroitin sulfate hematopoiesis immunosuppression Medicine R Pharmacy and materia medica RS1-441 Nadezhda E. Ustyuzhanina Natalia Yu. Anisimova Maria I. Bilan Fedor V. Donenko Galina E. Morozevich Dmitriy V. Yashunskiy Anatolii I. Usov Nara G. Siminyan Kirill I. Kirgisov Svetlana R. Varfolomeeva Mikhail V. Kiselevskiy Nikolay E. Nifantiev Chondroitin Sulfate and Fucosylated Chondroitin Sulfate as Stimulators of Hematopoiesis in Cyclophosphamide-Induced Mice |
topic_facet |
graft versus host disease granulocyte colony-stimulating factor chondroitin sulfate fucosylated chondroitin sulfate hematopoiesis immunosuppression Medicine R Pharmacy and materia medica RS1-441 |
description |
The immunosuppression and inhibition of hematopoiesis are considered to be reasons for the development of complications after intensive chemotherapy and allogeneic hematopoietic stem cell transplantation. Chondroitin sulfate ( CS ), isolated from the fish Salmo salar , and fucosylated chondroitin sulfate ( FCS ), isolated from the sea cucumber Apostichopus japonicus , were studied for their roles as stimulators of hematopoiesis in a model of cyclophosphamide-induced immunosuppression in mice. The recombinant protein r G-CSF was applied as a reference. The studied polysaccharides were shown to stimulate the release of white and red blood cells, as well as platelets from bone marrow in immunosuppressed mice, while r G-CSF was only responsible for the significant increase in the level of leucocytes. The analysis of different populations of leucocytes in blood indicated that r G-CSF mainly stimulated the production of neutrophils, whereas in the cases of the studied saccharides, increases in the levels of monocytes, lymphocytes and neutrophils were observed. The normalization of the level of the pro-inflammatory cytokine IL-6 in the serum and the recovery of cell populations in the spleen were observed in immunosuppressed mice following treatment with the polysaccharides. An increase in the proliferative activity of hematopoietic cells CD34(+)CD45(+) was observed following ex vivo polysaccharide exposure. Further study on related oligosaccharides regarding their potential as promising drugs in the complex prophylaxis and therapy of hematopoiesis inhibition after intensive chemotherapy and allogeneic hematopoietic stem cell transplantation seems to be warranted. |
format |
Article in Journal/Newspaper |
author |
Nadezhda E. Ustyuzhanina Natalia Yu. Anisimova Maria I. Bilan Fedor V. Donenko Galina E. Morozevich Dmitriy V. Yashunskiy Anatolii I. Usov Nara G. Siminyan Kirill I. Kirgisov Svetlana R. Varfolomeeva Mikhail V. Kiselevskiy Nikolay E. Nifantiev |
author_facet |
Nadezhda E. Ustyuzhanina Natalia Yu. Anisimova Maria I. Bilan Fedor V. Donenko Galina E. Morozevich Dmitriy V. Yashunskiy Anatolii I. Usov Nara G. Siminyan Kirill I. Kirgisov Svetlana R. Varfolomeeva Mikhail V. Kiselevskiy Nikolay E. Nifantiev |
author_sort |
Nadezhda E. Ustyuzhanina |
title |
Chondroitin Sulfate and Fucosylated Chondroitin Sulfate as Stimulators of Hematopoiesis in Cyclophosphamide-Induced Mice |
title_short |
Chondroitin Sulfate and Fucosylated Chondroitin Sulfate as Stimulators of Hematopoiesis in Cyclophosphamide-Induced Mice |
title_full |
Chondroitin Sulfate and Fucosylated Chondroitin Sulfate as Stimulators of Hematopoiesis in Cyclophosphamide-Induced Mice |
title_fullStr |
Chondroitin Sulfate and Fucosylated Chondroitin Sulfate as Stimulators of Hematopoiesis in Cyclophosphamide-Induced Mice |
title_full_unstemmed |
Chondroitin Sulfate and Fucosylated Chondroitin Sulfate as Stimulators of Hematopoiesis in Cyclophosphamide-Induced Mice |
title_sort |
chondroitin sulfate and fucosylated chondroitin sulfate as stimulators of hematopoiesis in cyclophosphamide-induced mice |
publisher |
MDPI AG |
publishDate |
2021 |
url |
https://doi.org/10.3390/ph14111074 https://doaj.org/article/7d890cf1cf0d4914a77618a120abbfa9 |
genre |
Salmo salar |
genre_facet |
Salmo salar |
op_source |
Pharmaceuticals, Vol 14, Iss 1074, p 1074 (2021) |
op_relation |
https://www.mdpi.com/1424-8247/14/11/1074 https://doaj.org/toc/1424-8247 doi:10.3390/ph14111074 1424-8247 https://doaj.org/article/7d890cf1cf0d4914a77618a120abbfa9 |
op_doi |
https://doi.org/10.3390/ph14111074 |
container_title |
Pharmaceuticals |
container_volume |
14 |
container_issue |
11 |
container_start_page |
1074 |
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1766182662029967360 |