Blow collection as a non-invasive method for measuring cortisol in the beluga (Delphinapterus leucas).
Non-invasive sampling techniques are increasingly being used to monitor glucocorticoids, such as cortisol, as indicators of stressor load and fitness in zoo and wildlife conservation, research and medicine. For cetaceans, exhaled breath condensate (blow) provides a unique sampling matrix for such pu...
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ftdoajarticles:oai:doaj.org/article:7d4d258e24ae46f99ac811185e98dcf6 2023-05-15T15:41:48+02:00 Blow collection as a non-invasive method for measuring cortisol in the beluga (Delphinapterus leucas). Laura A Thompson Tracey R Spoon Caroline E C Goertz Roderick C Hobbs Tracy A Romano 2014-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0114062 https://doaj.org/article/7d4d258e24ae46f99ac811185e98dcf6 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4252093?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0114062 https://doaj.org/article/7d4d258e24ae46f99ac811185e98dcf6 PLoS ONE, Vol 9, Iss 12, p e114062 (2014) Medicine R Science Q article 2014 ftdoajarticles https://doi.org/10.1371/journal.pone.0114062 2022-12-31T04:53:21Z Non-invasive sampling techniques are increasingly being used to monitor glucocorticoids, such as cortisol, as indicators of stressor load and fitness in zoo and wildlife conservation, research and medicine. For cetaceans, exhaled breath condensate (blow) provides a unique sampling matrix for such purposes. The purpose of this work was to develop an appropriate collection methodology and validate the use of a commercially available EIA for measuring cortisol in blow samples collected from belugas (Delphinapterus leucas). Nitex membrane stretched over a petri dish provided the optimal method for collecting blow. A commercially available cortisol EIA for measuring human cortisol (detection limit 35 pg ml-1) was adapted and validated for beluga cortisol using tests of parallelism, accuracy and recovery. Blow samples were collected from aquarium belugas during monthly health checks and during out of water examination, as well as from wild belugas. Two aquarium belugas showed increased blow cortisol between baseline samples and 30 minutes out of water (Baseline, 0.21 and 0.04 µg dl-1; 30 minutes, 0.95 and 0.14 µg dl-1). Six wild belugas also showed increases in blow cortisol between pre and post 1.5 hour examination (Pre 0.03, 0.23, 0.13, 0.19, 0.13, 0.04 µg dl-1, Post 0.60, 0.31, 0.36, 0.24, 0.14, 0.16 µg dl-1). Though this methodology needs further investigation, this study suggests that blow sampling is a good candidate for non-invasive monitoring of cortisol in belugas. It can be collected from both wild and aquarium animals efficiently for the purposes of health monitoring and research, and may ultimately be useful in obtaining data on wild populations, including endangered species, which are difficult to handle directly. Article in Journal/Newspaper Beluga Beluga* Delphinapterus leucas Directory of Open Access Journals: DOAJ Articles Eia ENVELOPE(7.755,7.755,63.024,63.024) PLoS ONE 9 12 e114062 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Medicine R Science Q |
spellingShingle |
Medicine R Science Q Laura A Thompson Tracey R Spoon Caroline E C Goertz Roderick C Hobbs Tracy A Romano Blow collection as a non-invasive method for measuring cortisol in the beluga (Delphinapterus leucas). |
topic_facet |
Medicine R Science Q |
description |
Non-invasive sampling techniques are increasingly being used to monitor glucocorticoids, such as cortisol, as indicators of stressor load and fitness in zoo and wildlife conservation, research and medicine. For cetaceans, exhaled breath condensate (blow) provides a unique sampling matrix for such purposes. The purpose of this work was to develop an appropriate collection methodology and validate the use of a commercially available EIA for measuring cortisol in blow samples collected from belugas (Delphinapterus leucas). Nitex membrane stretched over a petri dish provided the optimal method for collecting blow. A commercially available cortisol EIA for measuring human cortisol (detection limit 35 pg ml-1) was adapted and validated for beluga cortisol using tests of parallelism, accuracy and recovery. Blow samples were collected from aquarium belugas during monthly health checks and during out of water examination, as well as from wild belugas. Two aquarium belugas showed increased blow cortisol between baseline samples and 30 minutes out of water (Baseline, 0.21 and 0.04 µg dl-1; 30 minutes, 0.95 and 0.14 µg dl-1). Six wild belugas also showed increases in blow cortisol between pre and post 1.5 hour examination (Pre 0.03, 0.23, 0.13, 0.19, 0.13, 0.04 µg dl-1, Post 0.60, 0.31, 0.36, 0.24, 0.14, 0.16 µg dl-1). Though this methodology needs further investigation, this study suggests that blow sampling is a good candidate for non-invasive monitoring of cortisol in belugas. It can be collected from both wild and aquarium animals efficiently for the purposes of health monitoring and research, and may ultimately be useful in obtaining data on wild populations, including endangered species, which are difficult to handle directly. |
format |
Article in Journal/Newspaper |
author |
Laura A Thompson Tracey R Spoon Caroline E C Goertz Roderick C Hobbs Tracy A Romano |
author_facet |
Laura A Thompson Tracey R Spoon Caroline E C Goertz Roderick C Hobbs Tracy A Romano |
author_sort |
Laura A Thompson |
title |
Blow collection as a non-invasive method for measuring cortisol in the beluga (Delphinapterus leucas). |
title_short |
Blow collection as a non-invasive method for measuring cortisol in the beluga (Delphinapterus leucas). |
title_full |
Blow collection as a non-invasive method for measuring cortisol in the beluga (Delphinapterus leucas). |
title_fullStr |
Blow collection as a non-invasive method for measuring cortisol in the beluga (Delphinapterus leucas). |
title_full_unstemmed |
Blow collection as a non-invasive method for measuring cortisol in the beluga (Delphinapterus leucas). |
title_sort |
blow collection as a non-invasive method for measuring cortisol in the beluga (delphinapterus leucas). |
publisher |
Public Library of Science (PLoS) |
publishDate |
2014 |
url |
https://doi.org/10.1371/journal.pone.0114062 https://doaj.org/article/7d4d258e24ae46f99ac811185e98dcf6 |
long_lat |
ENVELOPE(7.755,7.755,63.024,63.024) |
geographic |
Eia |
geographic_facet |
Eia |
genre |
Beluga Beluga* Delphinapterus leucas |
genre_facet |
Beluga Beluga* Delphinapterus leucas |
op_source |
PLoS ONE, Vol 9, Iss 12, p e114062 (2014) |
op_relation |
http://europepmc.org/articles/PMC4252093?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0114062 https://doaj.org/article/7d4d258e24ae46f99ac811185e98dcf6 |
op_doi |
https://doi.org/10.1371/journal.pone.0114062 |
container_title |
PLoS ONE |
container_volume |
9 |
container_issue |
12 |
container_start_page |
e114062 |
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