Viral Protein Kinetics of Piscine Orthoreovirus Infection in Atlantic Salmon Blood Cells
Piscine orthoreovirus (PRV) is ubiquitous in farmed Atlantic salmon (Salmo salar) and the cause of heart and skeletal muscle inflammation. Erythrocytes are important target cells for PRV. We have investigated the kinetics of PRV infection in salmon blood cells. The findings indicate that PRV causes...
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ftdoajarticles:oai:doaj.org/article:7d4ace7d62b04be9ab5054c227c84c10 2023-05-15T15:30:44+02:00 Viral Protein Kinetics of Piscine Orthoreovirus Infection in Atlantic Salmon Blood Cells Hanne Merethe Haatveit Øystein Wessel Turhan Markussen Morten Lund Bernd Thiede Ingvild Berg Nyman Stine Braaen Maria Krudtaa Dahle Espen Rimstad 2017-03-01T00:00:00Z https://doi.org/10.3390/v9030049 https://doaj.org/article/7d4ace7d62b04be9ab5054c227c84c10 EN eng MDPI AG http://www.mdpi.com/1999-4915/9/3/49 https://doaj.org/toc/1999-4915 1999-4915 doi:10.3390/v9030049 https://doaj.org/article/7d4ace7d62b04be9ab5054c227c84c10 Viruses, Vol 9, Iss 3, p 49 (2017) Piscine orthoreovirus PRV non-structural protein µNS µ1 expression kinetics proteolytic cleavage pathogenesis blood cells Atlantic Salmon Microbiology QR1-502 article 2017 ftdoajarticles https://doi.org/10.3390/v9030049 2022-12-31T05:14:22Z Piscine orthoreovirus (PRV) is ubiquitous in farmed Atlantic salmon (Salmo salar) and the cause of heart and skeletal muscle inflammation. Erythrocytes are important target cells for PRV. We have investigated the kinetics of PRV infection in salmon blood cells. The findings indicate that PRV causes an acute infection of blood cells lasting 1–2 weeks, before it subsides into persistence. A high production of viral proteins occurred initially in the acute phase which significantly correlated with antiviral gene transcription. Globular viral factories organized by the non-structural protein µNS were also observed initially, but were not evident at later stages. Interactions between µNS and the PRV structural proteins λ1, µ1, σ1 and σ3 were demonstrated. Different size variants of µNS and the outer capsid protein µ1 appeared at specific time points during infection. Maximal viral protein load was observed five weeks post cohabitant challenge and was undetectable from seven weeks post challenge. In contrast, viral RNA at a high level could be detected throughout the eight-week trial. A proteolytic cleavage fragment of the µ1 protein was the only viral protein detectable after seven weeks post challenge, indicating that this µ1 fragment may be involved in the mechanisms of persistent infection. Article in Journal/Newspaper Atlantic salmon Salmo salar Directory of Open Access Journals: DOAJ Articles Viruses 9 3 49 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Piscine orthoreovirus PRV non-structural protein µNS µ1 expression kinetics proteolytic cleavage pathogenesis blood cells Atlantic Salmon Microbiology QR1-502 |
spellingShingle |
Piscine orthoreovirus PRV non-structural protein µNS µ1 expression kinetics proteolytic cleavage pathogenesis blood cells Atlantic Salmon Microbiology QR1-502 Hanne Merethe Haatveit Øystein Wessel Turhan Markussen Morten Lund Bernd Thiede Ingvild Berg Nyman Stine Braaen Maria Krudtaa Dahle Espen Rimstad Viral Protein Kinetics of Piscine Orthoreovirus Infection in Atlantic Salmon Blood Cells |
topic_facet |
Piscine orthoreovirus PRV non-structural protein µNS µ1 expression kinetics proteolytic cleavage pathogenesis blood cells Atlantic Salmon Microbiology QR1-502 |
description |
Piscine orthoreovirus (PRV) is ubiquitous in farmed Atlantic salmon (Salmo salar) and the cause of heart and skeletal muscle inflammation. Erythrocytes are important target cells for PRV. We have investigated the kinetics of PRV infection in salmon blood cells. The findings indicate that PRV causes an acute infection of blood cells lasting 1–2 weeks, before it subsides into persistence. A high production of viral proteins occurred initially in the acute phase which significantly correlated with antiviral gene transcription. Globular viral factories organized by the non-structural protein µNS were also observed initially, but were not evident at later stages. Interactions between µNS and the PRV structural proteins λ1, µ1, σ1 and σ3 were demonstrated. Different size variants of µNS and the outer capsid protein µ1 appeared at specific time points during infection. Maximal viral protein load was observed five weeks post cohabitant challenge and was undetectable from seven weeks post challenge. In contrast, viral RNA at a high level could be detected throughout the eight-week trial. A proteolytic cleavage fragment of the µ1 protein was the only viral protein detectable after seven weeks post challenge, indicating that this µ1 fragment may be involved in the mechanisms of persistent infection. |
format |
Article in Journal/Newspaper |
author |
Hanne Merethe Haatveit Øystein Wessel Turhan Markussen Morten Lund Bernd Thiede Ingvild Berg Nyman Stine Braaen Maria Krudtaa Dahle Espen Rimstad |
author_facet |
Hanne Merethe Haatveit Øystein Wessel Turhan Markussen Morten Lund Bernd Thiede Ingvild Berg Nyman Stine Braaen Maria Krudtaa Dahle Espen Rimstad |
author_sort |
Hanne Merethe Haatveit |
title |
Viral Protein Kinetics of Piscine Orthoreovirus Infection in Atlantic Salmon Blood Cells |
title_short |
Viral Protein Kinetics of Piscine Orthoreovirus Infection in Atlantic Salmon Blood Cells |
title_full |
Viral Protein Kinetics of Piscine Orthoreovirus Infection in Atlantic Salmon Blood Cells |
title_fullStr |
Viral Protein Kinetics of Piscine Orthoreovirus Infection in Atlantic Salmon Blood Cells |
title_full_unstemmed |
Viral Protein Kinetics of Piscine Orthoreovirus Infection in Atlantic Salmon Blood Cells |
title_sort |
viral protein kinetics of piscine orthoreovirus infection in atlantic salmon blood cells |
publisher |
MDPI AG |
publishDate |
2017 |
url |
https://doi.org/10.3390/v9030049 https://doaj.org/article/7d4ace7d62b04be9ab5054c227c84c10 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Viruses, Vol 9, Iss 3, p 49 (2017) |
op_relation |
http://www.mdpi.com/1999-4915/9/3/49 https://doaj.org/toc/1999-4915 1999-4915 doi:10.3390/v9030049 https://doaj.org/article/7d4ace7d62b04be9ab5054c227c84c10 |
op_doi |
https://doi.org/10.3390/v9030049 |
container_title |
Viruses |
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9 |
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3 |
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49 |
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1766361195584946176 |