Respuesta humoral IgM e IgG frente a antigenos de excreción-secreción de Schistosoma mansoni en murinos infectados y su relación con las citoquinas Il-10 y TNF-a

• Main Authors: Ochoa U Genesis, Mujica S Andrea, Barrios Emilia E, Cosenza C Miguel, Capellán M Radhames, Ayala Jennifer, Ojeda G Olga
• Format: Article in Journal/Newspaper
• Language: English; Spanish
• Published: Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales 2015
• Subjects: Schistosoma mansoni; respuesta Inmune; citoquinas pro-inflamatorias; respuesta granulomatosa; inmune response; pro-inflammatorycitokines; granulomatous response; Arctic medicine. Tropical medicine; RC955-962; Public aspects of medicine; RA1-1270; Arctic; Huevo
• Online Access: https://doaj.org/article/7cc885dc6c2b4780956a786201408d1d

Abstract: The diagnosis of schistosomiasis phase is complex. The murine IgM and IgG response against excretory-secretory products of Schistosomamansonimales (PESGM), females (PESGH) and eggs (PESH), its relation to histopathology and expression of IL-10 and TNF-a was assessed by ELISA in serum of Balb/c mice with 8 and 20 weeks of infection (RI8 and RI20) and healthy mice, RS. In RI8, were observed granulomas consisting of plasma cells, macrophages and neutrophils, deposits of collagen around the granulomas and internal area of the egg. In R20SI, fibroblasts around the egg and accumulation of macrophages and plasmocito, increased collagen deposits in areas of granuloma were observed.RI8 serum IgM had a higher percentage of positivity PESGH (35%), while the highest percentage of IgG positivity was PESGH (60%) and PESH (30%). In R20SI, was 20% IgM positive and IgG against PESH PESGM 10% against and 25% positive with PESGH. No differences in IL-10 between the RS and RI8 were observed. TNF-a in RS vs RI8 and RS vs RI20 was different and statistically significant. The PESGH could detect phase acute while PESH chronic phase. The use of several antigens would be useful in the diagnosis phase. Resumen: El diagnóstico de fase en la esquistosomiasis es complejo. Se evaluó la respuesta IgM e IgGmurina contra productos de excreción-secreción de Schistosomamansoni machos (PESGM), hembras (PESGH) y huevos (PESH), su relación con la histopatología y expresión de IL-10 y TNF-a, mediante ELISA, en suero de ratones Balb/c con 8 y 20 semanas de infección (RI8 y RI20) y Ratones sanos(RS). En RI8, se observaron granulomas constituidos por plasmocitos, macrófagos y neutrófilos, depósitos de colágeno alrededor de los granulomas y en la zona interna del huevo. En R20SI, se observaron fibroblastos alrededor del huevo y acúmulos de macrófagos y plasmocito, aumento de los depósitos de colágeno en áreas del granuloma. IgM sérica RI8, presentó un mayor porcentaje de positividad frente PESGH (35%), mientras que IgG el mayor porcentaje de ...