Canine Visceral Leishmaniasis in Wild Canines (Fox, Jackal, and Wolf) in Northeastern Iran Using Parasitological, Serological, and Molecular Methods
Background: Although many studies had been conducted on various aspects of canine visceral leishmaniasis (CVL) in domestic dogs in the endemic areas of Iran, investigations on CVL in wild canines are rare. Methods: This is a cross-sectional study was conducted from December 2012 to 2013 in northeast...
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Tehran University of Medical Sciences
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ftdoajarticles:oai:doaj.org/article:7cb0c2bac1774ddb8859cfc937dfb14d 2023-05-15T15:50:44+02:00 Canine Visceral Leishmaniasis in Wild Canines (Fox, Jackal, and Wolf) in Northeastern Iran Using Parasitological, Serological, and Molecular Methods Mehdi Mohebali Kourosh Arzamani Zabiholah Zarei Behnaz Akhoundi Homa Hajjaran Saber Raeghi Zahra Heidari Seyed Mousa Motavalli-Haghi Samira Elikaee Ahmad Mousazadeh-Mojarrad Zahra Kakoei 2016-10-01T00:00:00Z https://doaj.org/article/7cb0c2bac1774ddb8859cfc937dfb14d EN eng Tehran University of Medical Sciences https://jad.tums.ac.ir/index.php/jad/article/view/340 https://doaj.org/toc/1735-7179 https://doaj.org/toc/2322-2271 1735-7179 2322-2271 https://doaj.org/article/7cb0c2bac1774ddb8859cfc937dfb14d Journal of Arthropod-Borne Diseases, Vol 10, Iss 4 (2016) Canine visceral leishmaniasis Wild canines Iran Pathology RB1-214 article 2016 ftdoajarticles 2022-12-31T08:20:43Z Background: Although many studies had been conducted on various aspects of canine visceral leishmaniasis (CVL) in domestic dogs in the endemic areas of Iran, investigations on CVL in wild canines are rare. Methods: This is a cross-sectional study was conducted from December 2012 to 2013 in northeast of Iran where human VL is endemic. Wild canines were trapped around the areas where human VL cases had been previously identified. Wild canines were collected and examined both clinically and serologically using direct agglutination test (DAT). Microscopically examinations were performed in all the seropositive wild canines for the presence of the amastigote form of Leishmania spp. Some Leishmania sp. which had been isolated from the spleens of wild canines, were examined analyzed by conventional PCR and sequencing techniques using α-tubulin and GAPDH genes. Results: Altogether, 84 wild canines including foxes (Vulpes vulpes, n=21), Jackals (Canis aureus, n=60) and wolves (Canis lupus, n=3) were collected. Four foxes and seven jackals showed anti-Leishmania infantum antibodies with titers of 1:320–1:20480 in DAT. Furthermore, one fox and one jackal were parasitologically (microscopy and culture) positive and L. infantum was confirmed by sequence analysis. Conclusion: The present study showed that sylvatic cycle of L. infantum had been established in the studied endemic areas of VL in northeastern Iran. Article in Journal/Newspaper Canis lupus Directory of Open Access Journals: DOAJ Articles |
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Directory of Open Access Journals: DOAJ Articles |
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Canine visceral leishmaniasis Wild canines Iran Pathology RB1-214 |
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Canine visceral leishmaniasis Wild canines Iran Pathology RB1-214 Mehdi Mohebali Kourosh Arzamani Zabiholah Zarei Behnaz Akhoundi Homa Hajjaran Saber Raeghi Zahra Heidari Seyed Mousa Motavalli-Haghi Samira Elikaee Ahmad Mousazadeh-Mojarrad Zahra Kakoei Canine Visceral Leishmaniasis in Wild Canines (Fox, Jackal, and Wolf) in Northeastern Iran Using Parasitological, Serological, and Molecular Methods |
topic_facet |
Canine visceral leishmaniasis Wild canines Iran Pathology RB1-214 |
description |
Background: Although many studies had been conducted on various aspects of canine visceral leishmaniasis (CVL) in domestic dogs in the endemic areas of Iran, investigations on CVL in wild canines are rare. Methods: This is a cross-sectional study was conducted from December 2012 to 2013 in northeast of Iran where human VL is endemic. Wild canines were trapped around the areas where human VL cases had been previously identified. Wild canines were collected and examined both clinically and serologically using direct agglutination test (DAT). Microscopically examinations were performed in all the seropositive wild canines for the presence of the amastigote form of Leishmania spp. Some Leishmania sp. which had been isolated from the spleens of wild canines, were examined analyzed by conventional PCR and sequencing techniques using α-tubulin and GAPDH genes. Results: Altogether, 84 wild canines including foxes (Vulpes vulpes, n=21), Jackals (Canis aureus, n=60) and wolves (Canis lupus, n=3) were collected. Four foxes and seven jackals showed anti-Leishmania infantum antibodies with titers of 1:320–1:20480 in DAT. Furthermore, one fox and one jackal were parasitologically (microscopy and culture) positive and L. infantum was confirmed by sequence analysis. Conclusion: The present study showed that sylvatic cycle of L. infantum had been established in the studied endemic areas of VL in northeastern Iran. |
format |
Article in Journal/Newspaper |
author |
Mehdi Mohebali Kourosh Arzamani Zabiholah Zarei Behnaz Akhoundi Homa Hajjaran Saber Raeghi Zahra Heidari Seyed Mousa Motavalli-Haghi Samira Elikaee Ahmad Mousazadeh-Mojarrad Zahra Kakoei |
author_facet |
Mehdi Mohebali Kourosh Arzamani Zabiholah Zarei Behnaz Akhoundi Homa Hajjaran Saber Raeghi Zahra Heidari Seyed Mousa Motavalli-Haghi Samira Elikaee Ahmad Mousazadeh-Mojarrad Zahra Kakoei |
author_sort |
Mehdi Mohebali |
title |
Canine Visceral Leishmaniasis in Wild Canines (Fox, Jackal, and Wolf) in Northeastern Iran Using Parasitological, Serological, and Molecular Methods |
title_short |
Canine Visceral Leishmaniasis in Wild Canines (Fox, Jackal, and Wolf) in Northeastern Iran Using Parasitological, Serological, and Molecular Methods |
title_full |
Canine Visceral Leishmaniasis in Wild Canines (Fox, Jackal, and Wolf) in Northeastern Iran Using Parasitological, Serological, and Molecular Methods |
title_fullStr |
Canine Visceral Leishmaniasis in Wild Canines (Fox, Jackal, and Wolf) in Northeastern Iran Using Parasitological, Serological, and Molecular Methods |
title_full_unstemmed |
Canine Visceral Leishmaniasis in Wild Canines (Fox, Jackal, and Wolf) in Northeastern Iran Using Parasitological, Serological, and Molecular Methods |
title_sort |
canine visceral leishmaniasis in wild canines (fox, jackal, and wolf) in northeastern iran using parasitological, serological, and molecular methods |
publisher |
Tehran University of Medical Sciences |
publishDate |
2016 |
url |
https://doaj.org/article/7cb0c2bac1774ddb8859cfc937dfb14d |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_source |
Journal of Arthropod-Borne Diseases, Vol 10, Iss 4 (2016) |
op_relation |
https://jad.tums.ac.ir/index.php/jad/article/view/340 https://doaj.org/toc/1735-7179 https://doaj.org/toc/2322-2271 1735-7179 2322-2271 https://doaj.org/article/7cb0c2bac1774ddb8859cfc937dfb14d |
_version_ |
1766385749042659328 |