Antarctic Moss Multiprotein Bridging Factor 1c Overexpression in Arabidopsis Resulted in Enhanced Tolerance to Salt Stress
Polytrichastrum alpinum is one of the moss species that survives extreme conditions in the Antarctic. In order to explore the functional benefits of moss genetic resources, P. alpinum multiprotein-bridging factor 1c gene (PaMBF1c) was isolated and characterized. The deduced amino acid sequence of Pa...
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ftdoajarticles:oai:doaj.org/article:7c4f200c8c55454fad59fee326e00c4c 2023-05-15T13:44:08+02:00 Antarctic Moss Multiprotein Bridging Factor 1c Overexpression in Arabidopsis Resulted in Enhanced Tolerance to Salt Stress Hemasundar Alavilli Hyoungseok Lee Mira Park Byeong-ha Lee 2017-07-01T00:00:00Z https://doi.org/10.3389/fpls.2017.01206 https://doaj.org/article/7c4f200c8c55454fad59fee326e00c4c EN eng Frontiers Media S.A. http://journal.frontiersin.org/article/10.3389/fpls.2017.01206/full https://doaj.org/toc/1664-462X 1664-462X doi:10.3389/fpls.2017.01206 https://doaj.org/article/7c4f200c8c55454fad59fee326e00c4c Frontiers in Plant Science, Vol 8 (2017) stress tolerance salt stress MBF1c RNA sequencing Antarctic moss Polytrichastrum alpinum Plant culture SB1-1110 article 2017 ftdoajarticles https://doi.org/10.3389/fpls.2017.01206 2022-12-31T11:47:51Z Polytrichastrum alpinum is one of the moss species that survives extreme conditions in the Antarctic. In order to explore the functional benefits of moss genetic resources, P. alpinum multiprotein-bridging factor 1c gene (PaMBF1c) was isolated and characterized. The deduced amino acid sequence of PaMBF1c comprises of a multiprotein-bridging factor (MBF1) domain and a helix-turn-helix (HTH) domain. PaMBF1c expression was induced by different abiotic stresses in P. alpinum, implying its roles in stress responses. We overexpressed PaMBF1c in Arabidopsis and analyzed the resulting phenotypes in comparison with wild type and/or Arabidopsis MBF1c (AtMBF1c) overexpressors. Overexpression of PaMBF1c in Arabidopsis resulted in enhanced tolerance to salt and osmotic stress, as well as to cold and heat stress. More specifically, enhanced salt tolerance was observed in PaMBF1c overexpressors in comparison to wild type but not clearly observable in AtMBF1c overexpressing lines. Thus, these results implicate the evolution of PaMBF1c under salt-enriched Antarctic soil. RNA-Seq profiling of NaCl-treated plants revealed that 10 salt-stress inducible genes were already up-regulated in PaMBF1c overexpressing plants even before NaCl treatment. Gene ontology enrichment analysis with salt up-regulated genes in each line uncovered that the terms lipid metabolic process, ion transport, and cellular amino acid biosynthetic process were significantly enriched in PaMBF1c overexpressors. Additionally, gene enrichment analysis with salt down-regulated genes in each line revealed that the enriched categories in wild type were not significantly overrepresented in PaMBF1c overexpressing lines. The up-regulation of several genes only in PaMBF1c overexpressing lines suggest that enhanced salt tolerance in PaMBF1c-OE might involve reactive oxygen species detoxification, maintenance of ATP homeostasis, and facilitation of Ca2+ signaling. Interestingly, many salt down-regulated ribosome- and translation-related genes were not down-regulated in ... Article in Journal/Newspaper Antarc* Antarctic Directory of Open Access Journals: DOAJ Articles Antarctic The Antarctic Frontiers in Plant Science 8 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
stress tolerance salt stress MBF1c RNA sequencing Antarctic moss Polytrichastrum alpinum Plant culture SB1-1110 |
spellingShingle |
stress tolerance salt stress MBF1c RNA sequencing Antarctic moss Polytrichastrum alpinum Plant culture SB1-1110 Hemasundar Alavilli Hyoungseok Lee Mira Park Byeong-ha Lee Antarctic Moss Multiprotein Bridging Factor 1c Overexpression in Arabidopsis Resulted in Enhanced Tolerance to Salt Stress |
topic_facet |
stress tolerance salt stress MBF1c RNA sequencing Antarctic moss Polytrichastrum alpinum Plant culture SB1-1110 |
description |
Polytrichastrum alpinum is one of the moss species that survives extreme conditions in the Antarctic. In order to explore the functional benefits of moss genetic resources, P. alpinum multiprotein-bridging factor 1c gene (PaMBF1c) was isolated and characterized. The deduced amino acid sequence of PaMBF1c comprises of a multiprotein-bridging factor (MBF1) domain and a helix-turn-helix (HTH) domain. PaMBF1c expression was induced by different abiotic stresses in P. alpinum, implying its roles in stress responses. We overexpressed PaMBF1c in Arabidopsis and analyzed the resulting phenotypes in comparison with wild type and/or Arabidopsis MBF1c (AtMBF1c) overexpressors. Overexpression of PaMBF1c in Arabidopsis resulted in enhanced tolerance to salt and osmotic stress, as well as to cold and heat stress. More specifically, enhanced salt tolerance was observed in PaMBF1c overexpressors in comparison to wild type but not clearly observable in AtMBF1c overexpressing lines. Thus, these results implicate the evolution of PaMBF1c under salt-enriched Antarctic soil. RNA-Seq profiling of NaCl-treated plants revealed that 10 salt-stress inducible genes were already up-regulated in PaMBF1c overexpressing plants even before NaCl treatment. Gene ontology enrichment analysis with salt up-regulated genes in each line uncovered that the terms lipid metabolic process, ion transport, and cellular amino acid biosynthetic process were significantly enriched in PaMBF1c overexpressors. Additionally, gene enrichment analysis with salt down-regulated genes in each line revealed that the enriched categories in wild type were not significantly overrepresented in PaMBF1c overexpressing lines. The up-regulation of several genes only in PaMBF1c overexpressing lines suggest that enhanced salt tolerance in PaMBF1c-OE might involve reactive oxygen species detoxification, maintenance of ATP homeostasis, and facilitation of Ca2+ signaling. Interestingly, many salt down-regulated ribosome- and translation-related genes were not down-regulated in ... |
format |
Article in Journal/Newspaper |
author |
Hemasundar Alavilli Hyoungseok Lee Mira Park Byeong-ha Lee |
author_facet |
Hemasundar Alavilli Hyoungseok Lee Mira Park Byeong-ha Lee |
author_sort |
Hemasundar Alavilli |
title |
Antarctic Moss Multiprotein Bridging Factor 1c Overexpression in Arabidopsis Resulted in Enhanced Tolerance to Salt Stress |
title_short |
Antarctic Moss Multiprotein Bridging Factor 1c Overexpression in Arabidopsis Resulted in Enhanced Tolerance to Salt Stress |
title_full |
Antarctic Moss Multiprotein Bridging Factor 1c Overexpression in Arabidopsis Resulted in Enhanced Tolerance to Salt Stress |
title_fullStr |
Antarctic Moss Multiprotein Bridging Factor 1c Overexpression in Arabidopsis Resulted in Enhanced Tolerance to Salt Stress |
title_full_unstemmed |
Antarctic Moss Multiprotein Bridging Factor 1c Overexpression in Arabidopsis Resulted in Enhanced Tolerance to Salt Stress |
title_sort |
antarctic moss multiprotein bridging factor 1c overexpression in arabidopsis resulted in enhanced tolerance to salt stress |
publisher |
Frontiers Media S.A. |
publishDate |
2017 |
url |
https://doi.org/10.3389/fpls.2017.01206 https://doaj.org/article/7c4f200c8c55454fad59fee326e00c4c |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Frontiers in Plant Science, Vol 8 (2017) |
op_relation |
http://journal.frontiersin.org/article/10.3389/fpls.2017.01206/full https://doaj.org/toc/1664-462X 1664-462X doi:10.3389/fpls.2017.01206 https://doaj.org/article/7c4f200c8c55454fad59fee326e00c4c |
op_doi |
https://doi.org/10.3389/fpls.2017.01206 |
container_title |
Frontiers in Plant Science |
container_volume |
8 |
_version_ |
1766198063985065984 |