Validation and implementation of GeneXpert MTB/RIF for diagnosis of Tuberculosis in Ecuador
The objective of the study was the validation and implementation of GeneXpert MTB/RIF for routine use in the rapid detection of tuberculosis and sensitivity to rifampicin in clinical samples; for this, 1592 respiratory samples were collected and analyzed in the laboratory of Instituto Nacional de In...
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Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales
2019
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ftdoajarticles:oai:doaj.org/article:7c44808e755840919a5049fefbc13dbb 2023-10-01T03:54:07+02:00 Validation and implementation of GeneXpert MTB/RIF for diagnosis of Tuberculosis in Ecuador José Marcelo Ortíz-Jimenes Greta Franco-Sotomayor Martha Ramos-Ramirez 2019-06-01T00:00:00Z https://doaj.org/article/7c44808e755840919a5049fefbc13dbb EN ES eng spa Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales http://produccioncientificaluz.org/index.php/kasmera/article/view/24674 https://doaj.org/toc/0075-5222 https://doaj.org/toc/2477-9628 0075-5222 2477-9628 https://doaj.org/article/7c44808e755840919a5049fefbc13dbb Kasmera, Vol 47, Iss 1, Pp 29-37 (2019) estudios de validación esputo sensibilidad especificidad rifampicina mycobacterium tuberculosis Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2019 ftdoajarticles 2023-09-03T00:34:45Z The objective of the study was the validation and implementation of GeneXpert MTB/RIF for routine use in the rapid detection of tuberculosis and sensitivity to rifampicin in clinical samples; for this, 1592 respiratory samples were collected and analyzed in the laboratory of Instituto Nacional de Investigación en Salud Pública Guayaquil. The analysis of the results of GeneXpert in comparison with smear microscopy showed an initial sensitivity of 99.8% and specificity of 93.2%; The analysis of discrepancies using the results of the culture as a reference method showed that the GeneXpert results considered false negatives turned out to be true negatives, the same happens with the false positives that correspond to true positives. Recalculated the sensitivity and specificity of the GeneXpert was 99.8% and 100% correspondingly. The comparison with the drugs susceptibility test showed a sensitivity of 91.4% and a specificity of 95.5% for the GeneXpert MTB/RIF system. It is concluded that the implementation of the GeneXpert system allows solution to certain problems associated with the application of conventional diagnostic methodologies, decreasing the waiting times, and increasing the sensitivity and specificity in the diagnosis of drug-resistant tuberculosis, thus generating a valuable opportunity for early diagnosis. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Guayaquil ENVELOPE(-59.767,-59.767,-62.449,-62.449) Rif ENVELOPE(-16.172,-16.172,66.526,66.526) |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English Spanish |
topic |
estudios de validación esputo sensibilidad especificidad rifampicina mycobacterium tuberculosis Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
estudios de validación esputo sensibilidad especificidad rifampicina mycobacterium tuberculosis Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 José Marcelo Ortíz-Jimenes Greta Franco-Sotomayor Martha Ramos-Ramirez Validation and implementation of GeneXpert MTB/RIF for diagnosis of Tuberculosis in Ecuador |
topic_facet |
estudios de validación esputo sensibilidad especificidad rifampicina mycobacterium tuberculosis Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
The objective of the study was the validation and implementation of GeneXpert MTB/RIF for routine use in the rapid detection of tuberculosis and sensitivity to rifampicin in clinical samples; for this, 1592 respiratory samples were collected and analyzed in the laboratory of Instituto Nacional de Investigación en Salud Pública Guayaquil. The analysis of the results of GeneXpert in comparison with smear microscopy showed an initial sensitivity of 99.8% and specificity of 93.2%; The analysis of discrepancies using the results of the culture as a reference method showed that the GeneXpert results considered false negatives turned out to be true negatives, the same happens with the false positives that correspond to true positives. Recalculated the sensitivity and specificity of the GeneXpert was 99.8% and 100% correspondingly. The comparison with the drugs susceptibility test showed a sensitivity of 91.4% and a specificity of 95.5% for the GeneXpert MTB/RIF system. It is concluded that the implementation of the GeneXpert system allows solution to certain problems associated with the application of conventional diagnostic methodologies, decreasing the waiting times, and increasing the sensitivity and specificity in the diagnosis of drug-resistant tuberculosis, thus generating a valuable opportunity for early diagnosis. |
format |
Article in Journal/Newspaper |
author |
José Marcelo Ortíz-Jimenes Greta Franco-Sotomayor Martha Ramos-Ramirez |
author_facet |
José Marcelo Ortíz-Jimenes Greta Franco-Sotomayor Martha Ramos-Ramirez |
author_sort |
José Marcelo Ortíz-Jimenes |
title |
Validation and implementation of GeneXpert MTB/RIF for diagnosis of Tuberculosis in Ecuador |
title_short |
Validation and implementation of GeneXpert MTB/RIF for diagnosis of Tuberculosis in Ecuador |
title_full |
Validation and implementation of GeneXpert MTB/RIF for diagnosis of Tuberculosis in Ecuador |
title_fullStr |
Validation and implementation of GeneXpert MTB/RIF for diagnosis of Tuberculosis in Ecuador |
title_full_unstemmed |
Validation and implementation of GeneXpert MTB/RIF for diagnosis of Tuberculosis in Ecuador |
title_sort |
validation and implementation of genexpert mtb/rif for diagnosis of tuberculosis in ecuador |
publisher |
Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales |
publishDate |
2019 |
url |
https://doaj.org/article/7c44808e755840919a5049fefbc13dbb |
long_lat |
ENVELOPE(-59.767,-59.767,-62.449,-62.449) ENVELOPE(-16.172,-16.172,66.526,66.526) |
geographic |
Arctic Guayaquil Rif |
geographic_facet |
Arctic Guayaquil Rif |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Kasmera, Vol 47, Iss 1, Pp 29-37 (2019) |
op_relation |
http://produccioncientificaluz.org/index.php/kasmera/article/view/24674 https://doaj.org/toc/0075-5222 https://doaj.org/toc/2477-9628 0075-5222 2477-9628 https://doaj.org/article/7c44808e755840919a5049fefbc13dbb |
_version_ |
1778521462191161344 |