Trogocytosis with monocytes associated with increased α2,3 sialic acid expression on B cells during H5N1 influenza virus infection
The immunopathogenesis of H5N1 virus has been studied intensively since it caused cross-species infection and induced high mortality to human. We previously observed the interaction between monocytes and B cells, which increased the susceptibility of B cell to H5N1 virus infection after a co-culture...
Main Authors: | , , , , , , , |
---|---|
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Public Library of Science (PLoS)
2020
|
Subjects: | |
Online Access: | https://doaj.org/article/7c32ee4648e741f59f0ea4b256824ccb |
id |
ftdoajarticles:oai:doaj.org/article:7c32ee4648e741f59f0ea4b256824ccb |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:7c32ee4648e741f59f0ea4b256824ccb 2023-05-15T15:34:18+02:00 Trogocytosis with monocytes associated with increased α2,3 sialic acid expression on B cells during H5N1 influenza virus infection Supasek Kongsomros Maytawan Thanunchai Suwimon Manopwisedjaroen Prasit Na-Ek Sheng-Fan Wang Tana Taechalertpaisarn Arunee Thitithanyanont Mathilde Richard 2020-01-01T00:00:00Z https://doaj.org/article/7c32ee4648e741f59f0ea4b256824ccb EN eng Public Library of Science (PLoS) https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7500609/?tool=EBI https://doaj.org/toc/1932-6203 1932-6203 https://doaj.org/article/7c32ee4648e741f59f0ea4b256824ccb PLoS ONE, Vol 15, Iss 9 (2020) Medicine R Science Q article 2020 ftdoajarticles 2022-12-31T11:46:13Z The immunopathogenesis of H5N1 virus has been studied intensively since it caused cross-species infection and induced high mortality to human. We previously observed the interaction between monocytes and B cells, which increased the susceptibility of B cell to H5N1 virus infection after a co-culture. Levels of α2,3 sialic acid (avian flu receptor) were also significantly increased on B cell surface in this co-culture model with unclear explanation. In this study, we aimed to determine the possible mechanism that responded for this increase in α2,3 sialic acid on B cells. Acquisition of α2,3 SA by B cells via cell contact-dependent trogocytosis was proposed. Results showed that the lack of α2,3 SA was detected on B cell surface, and B cells acquired membrane-bound α2,3 SA molecules from monocytes in H5N1-infected co-cultures. Occurrence of membrane exchange mainly relied on H5N1 infection and cell-cell contact as opposed to a mock infection and transwell. The increase in α2,3 SA on B cell surface mediated by trogocytosis was associated with the enhanced susceptibility to H5N1 infection. These observations thus provide the evidence that H5N1 influenza virus may utilize trogocytosis to expand its cell tropism and spread to immune cells despite the lack of avian flu receptor. Article in Journal/Newspaper Avian flu Directory of Open Access Journals: DOAJ Articles |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Medicine R Science Q |
spellingShingle |
Medicine R Science Q Supasek Kongsomros Maytawan Thanunchai Suwimon Manopwisedjaroen Prasit Na-Ek Sheng-Fan Wang Tana Taechalertpaisarn Arunee Thitithanyanont Mathilde Richard Trogocytosis with monocytes associated with increased α2,3 sialic acid expression on B cells during H5N1 influenza virus infection |
topic_facet |
Medicine R Science Q |
description |
The immunopathogenesis of H5N1 virus has been studied intensively since it caused cross-species infection and induced high mortality to human. We previously observed the interaction between monocytes and B cells, which increased the susceptibility of B cell to H5N1 virus infection after a co-culture. Levels of α2,3 sialic acid (avian flu receptor) were also significantly increased on B cell surface in this co-culture model with unclear explanation. In this study, we aimed to determine the possible mechanism that responded for this increase in α2,3 sialic acid on B cells. Acquisition of α2,3 SA by B cells via cell contact-dependent trogocytosis was proposed. Results showed that the lack of α2,3 SA was detected on B cell surface, and B cells acquired membrane-bound α2,3 SA molecules from monocytes in H5N1-infected co-cultures. Occurrence of membrane exchange mainly relied on H5N1 infection and cell-cell contact as opposed to a mock infection and transwell. The increase in α2,3 SA on B cell surface mediated by trogocytosis was associated with the enhanced susceptibility to H5N1 infection. These observations thus provide the evidence that H5N1 influenza virus may utilize trogocytosis to expand its cell tropism and spread to immune cells despite the lack of avian flu receptor. |
format |
Article in Journal/Newspaper |
author |
Supasek Kongsomros Maytawan Thanunchai Suwimon Manopwisedjaroen Prasit Na-Ek Sheng-Fan Wang Tana Taechalertpaisarn Arunee Thitithanyanont Mathilde Richard |
author_facet |
Supasek Kongsomros Maytawan Thanunchai Suwimon Manopwisedjaroen Prasit Na-Ek Sheng-Fan Wang Tana Taechalertpaisarn Arunee Thitithanyanont Mathilde Richard |
author_sort |
Supasek Kongsomros |
title |
Trogocytosis with monocytes associated with increased α2,3 sialic acid expression on B cells during H5N1 influenza virus infection |
title_short |
Trogocytosis with monocytes associated with increased α2,3 sialic acid expression on B cells during H5N1 influenza virus infection |
title_full |
Trogocytosis with monocytes associated with increased α2,3 sialic acid expression on B cells during H5N1 influenza virus infection |
title_fullStr |
Trogocytosis with monocytes associated with increased α2,3 sialic acid expression on B cells during H5N1 influenza virus infection |
title_full_unstemmed |
Trogocytosis with monocytes associated with increased α2,3 sialic acid expression on B cells during H5N1 influenza virus infection |
title_sort |
trogocytosis with monocytes associated with increased α2,3 sialic acid expression on b cells during h5n1 influenza virus infection |
publisher |
Public Library of Science (PLoS) |
publishDate |
2020 |
url |
https://doaj.org/article/7c32ee4648e741f59f0ea4b256824ccb |
genre |
Avian flu |
genre_facet |
Avian flu |
op_source |
PLoS ONE, Vol 15, Iss 9 (2020) |
op_relation |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7500609/?tool=EBI https://doaj.org/toc/1932-6203 1932-6203 https://doaj.org/article/7c32ee4648e741f59f0ea4b256824ccb |
_version_ |
1766364750329937920 |