Proteomic Profiling Skin Mucus of European Eel Anguilla anguilla Infected with Anguillid Herpesvirus
Anguillid herpesvirus 1 (AngHV) is an important viral pathogen affecting eel. This study was designed to investigate the potential molecular mechanisms and immune response elicited at the protein levels in the skin mucus of AngHV-infected Anguilla anguilla . Tandem mass tag (TMT)-labelling proteomic...
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ftdoajarticles:oai:doaj.org/article:7c2b4652e5034f799a06c6dbc0be4783 2023-05-15T13:26:45+02:00 Proteomic Profiling Skin Mucus of European Eel Anguilla anguilla Infected with Anguillid Herpesvirus Ying-Ying Li Jin-Xian Yang Xi Chen Qiang Chen Tie-Ying Song Jun-Qing Ge 2022-09-01T00:00:00Z https://doi.org/10.3390/ijms231911283 https://doaj.org/article/7c2b4652e5034f799a06c6dbc0be4783 EN eng MDPI AG https://www.mdpi.com/1422-0067/23/19/11283 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067 doi:10.3390/ijms231911283 1422-0067 1661-6596 https://doaj.org/article/7c2b4652e5034f799a06c6dbc0be4783 International Journal of Molecular Sciences, Vol 23, Iss 11283, p 11283 (2022) Anguilla anguilla Anguillid herpesvirus TMT-labelled proteomic skin mucus Biology (General) QH301-705.5 Chemistry QD1-999 article 2022 ftdoajarticles https://doi.org/10.3390/ijms231911283 2022-12-30T19:46:48Z Anguillid herpesvirus 1 (AngHV) is an important viral pathogen affecting eel. This study was designed to investigate the potential molecular mechanisms and immune response elicited at the protein levels in the skin mucus of AngHV-infected Anguilla anguilla . Tandem mass tag (TMT)-labelling proteomics with the liquid chromatography tandem mass spectrometry (LC-MS/MS) was used for performing quantitative identification of the proteins. In addition, the quantitative protein amount was detected by parallel reaction monitoring (PRM) analysis. A total of 3486 proteins were identified, of which 2935 were quantified. When a protein fold change was greater than 1.3 or less than 0.76, it indicated a differentially expressed protein (DEP). Overall, 187 up-regulated proteins and 126 down-regulated proteins were detected, and most of the DEPs were enriched in the CAMs pathway, intestinal immune pathway, herpes simplex virus 1 infection pathway, phagosome pathway and p53 signaling pathway. The results of the DEPs detected by PRM were highly consistent with the results of the TMT-labelled quantitative proteomic analysis. The findings of this study provide an important research basis for further understanding the pathogenesis of AngHV. Article in Journal/Newspaper Anguilla anguilla Directory of Open Access Journals: DOAJ Articles International Journal of Molecular Sciences 23 19 11283 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Anguilla anguilla Anguillid herpesvirus TMT-labelled proteomic skin mucus Biology (General) QH301-705.5 Chemistry QD1-999 |
spellingShingle |
Anguilla anguilla Anguillid herpesvirus TMT-labelled proteomic skin mucus Biology (General) QH301-705.5 Chemistry QD1-999 Ying-Ying Li Jin-Xian Yang Xi Chen Qiang Chen Tie-Ying Song Jun-Qing Ge Proteomic Profiling Skin Mucus of European Eel Anguilla anguilla Infected with Anguillid Herpesvirus |
topic_facet |
Anguilla anguilla Anguillid herpesvirus TMT-labelled proteomic skin mucus Biology (General) QH301-705.5 Chemistry QD1-999 |
description |
Anguillid herpesvirus 1 (AngHV) is an important viral pathogen affecting eel. This study was designed to investigate the potential molecular mechanisms and immune response elicited at the protein levels in the skin mucus of AngHV-infected Anguilla anguilla . Tandem mass tag (TMT)-labelling proteomics with the liquid chromatography tandem mass spectrometry (LC-MS/MS) was used for performing quantitative identification of the proteins. In addition, the quantitative protein amount was detected by parallel reaction monitoring (PRM) analysis. A total of 3486 proteins were identified, of which 2935 were quantified. When a protein fold change was greater than 1.3 or less than 0.76, it indicated a differentially expressed protein (DEP). Overall, 187 up-regulated proteins and 126 down-regulated proteins were detected, and most of the DEPs were enriched in the CAMs pathway, intestinal immune pathway, herpes simplex virus 1 infection pathway, phagosome pathway and p53 signaling pathway. The results of the DEPs detected by PRM were highly consistent with the results of the TMT-labelled quantitative proteomic analysis. The findings of this study provide an important research basis for further understanding the pathogenesis of AngHV. |
format |
Article in Journal/Newspaper |
author |
Ying-Ying Li Jin-Xian Yang Xi Chen Qiang Chen Tie-Ying Song Jun-Qing Ge |
author_facet |
Ying-Ying Li Jin-Xian Yang Xi Chen Qiang Chen Tie-Ying Song Jun-Qing Ge |
author_sort |
Ying-Ying Li |
title |
Proteomic Profiling Skin Mucus of European Eel Anguilla anguilla Infected with Anguillid Herpesvirus |
title_short |
Proteomic Profiling Skin Mucus of European Eel Anguilla anguilla Infected with Anguillid Herpesvirus |
title_full |
Proteomic Profiling Skin Mucus of European Eel Anguilla anguilla Infected with Anguillid Herpesvirus |
title_fullStr |
Proteomic Profiling Skin Mucus of European Eel Anguilla anguilla Infected with Anguillid Herpesvirus |
title_full_unstemmed |
Proteomic Profiling Skin Mucus of European Eel Anguilla anguilla Infected with Anguillid Herpesvirus |
title_sort |
proteomic profiling skin mucus of european eel anguilla anguilla infected with anguillid herpesvirus |
publisher |
MDPI AG |
publishDate |
2022 |
url |
https://doi.org/10.3390/ijms231911283 https://doaj.org/article/7c2b4652e5034f799a06c6dbc0be4783 |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_source |
International Journal of Molecular Sciences, Vol 23, Iss 11283, p 11283 (2022) |
op_relation |
https://www.mdpi.com/1422-0067/23/19/11283 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067 doi:10.3390/ijms231911283 1422-0067 1661-6596 https://doaj.org/article/7c2b4652e5034f799a06c6dbc0be4783 |
op_doi |
https://doi.org/10.3390/ijms231911283 |
container_title |
International Journal of Molecular Sciences |
container_volume |
23 |
container_issue |
19 |
container_start_page |
11283 |
_version_ |
1766394279728513024 |