Isolation of saint louis encephalitis virus from a horse with neurological disease in Brazil.
St. Louis encephalitis virus (SLEV) is a causative agent of encephalitis in humans in the Western hemisphere. SLEV is a positive-sense RNA virus that belongs to the Flavivirus genus, which includes West Nile encephalitis virus, Japanese encephalitis virus, Dengue virus and other medically important...
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ftdoajarticles:oai:doaj.org/article:7c126646a3c84c6391b676c528e0682a 2023-05-15T15:04:32+02:00 Isolation of saint louis encephalitis virus from a horse with neurological disease in Brazil. Roberta Rosa Erica Azevedo Costa Rafael Elias Marques Taismara Simas Oliveira Ronaldo Furtini Maria Rosa Quaresma Bomfim Mauro Martins Teixeira Tatiane Alves Paixão Renato Lima Santos 2013-11-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0002537 https://doaj.org/article/7c126646a3c84c6391b676c528e0682a EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3836713?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002537 https://doaj.org/article/7c126646a3c84c6391b676c528e0682a PLoS Neglected Tropical Diseases, Vol 7, Iss 11, p e2537 (2013) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2013 ftdoajarticles https://doi.org/10.1371/journal.pntd.0002537 2022-12-31T01:28:59Z St. Louis encephalitis virus (SLEV) is a causative agent of encephalitis in humans in the Western hemisphere. SLEV is a positive-sense RNA virus that belongs to the Flavivirus genus, which includes West Nile encephalitis virus, Japanese encephalitis virus, Dengue virus and other medically important viruses. Recently, we isolated a SLEV strain from the brain of a horse with neurological signs in the countryside of Minas Gerais, Brazil. The SLEV isolation was confirmed by reverse-transcription RT-PCR and sequencing of the E protein gene. Virus identity was also confirmed by indirect immunofluorescence using commercial antibodies against SLEV. To characterize this newly isolated strain in vivo, serial passages in newborn mice were performed and led to hemorrhagic manifestations associated with recruitment of inflammatory cells into the central nervous system of newborns. In summary this is the first isolation of SLEV from a horse with neurological signs in Brazil. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic St. Louis ENVELOPE(-67.496,-67.496,-67.132,-67.132) PLoS Neglected Tropical Diseases 7 11 e2537 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Roberta Rosa Erica Azevedo Costa Rafael Elias Marques Taismara Simas Oliveira Ronaldo Furtini Maria Rosa Quaresma Bomfim Mauro Martins Teixeira Tatiane Alves Paixão Renato Lima Santos Isolation of saint louis encephalitis virus from a horse with neurological disease in Brazil. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
St. Louis encephalitis virus (SLEV) is a causative agent of encephalitis in humans in the Western hemisphere. SLEV is a positive-sense RNA virus that belongs to the Flavivirus genus, which includes West Nile encephalitis virus, Japanese encephalitis virus, Dengue virus and other medically important viruses. Recently, we isolated a SLEV strain from the brain of a horse with neurological signs in the countryside of Minas Gerais, Brazil. The SLEV isolation was confirmed by reverse-transcription RT-PCR and sequencing of the E protein gene. Virus identity was also confirmed by indirect immunofluorescence using commercial antibodies against SLEV. To characterize this newly isolated strain in vivo, serial passages in newborn mice were performed and led to hemorrhagic manifestations associated with recruitment of inflammatory cells into the central nervous system of newborns. In summary this is the first isolation of SLEV from a horse with neurological signs in Brazil. |
format |
Article in Journal/Newspaper |
author |
Roberta Rosa Erica Azevedo Costa Rafael Elias Marques Taismara Simas Oliveira Ronaldo Furtini Maria Rosa Quaresma Bomfim Mauro Martins Teixeira Tatiane Alves Paixão Renato Lima Santos |
author_facet |
Roberta Rosa Erica Azevedo Costa Rafael Elias Marques Taismara Simas Oliveira Ronaldo Furtini Maria Rosa Quaresma Bomfim Mauro Martins Teixeira Tatiane Alves Paixão Renato Lima Santos |
author_sort |
Roberta Rosa |
title |
Isolation of saint louis encephalitis virus from a horse with neurological disease in Brazil. |
title_short |
Isolation of saint louis encephalitis virus from a horse with neurological disease in Brazil. |
title_full |
Isolation of saint louis encephalitis virus from a horse with neurological disease in Brazil. |
title_fullStr |
Isolation of saint louis encephalitis virus from a horse with neurological disease in Brazil. |
title_full_unstemmed |
Isolation of saint louis encephalitis virus from a horse with neurological disease in Brazil. |
title_sort |
isolation of saint louis encephalitis virus from a horse with neurological disease in brazil. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2013 |
url |
https://doi.org/10.1371/journal.pntd.0002537 https://doaj.org/article/7c126646a3c84c6391b676c528e0682a |
long_lat |
ENVELOPE(-67.496,-67.496,-67.132,-67.132) |
geographic |
Arctic St. Louis |
geographic_facet |
Arctic St. Louis |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 7, Iss 11, p e2537 (2013) |
op_relation |
http://europepmc.org/articles/PMC3836713?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002537 https://doaj.org/article/7c126646a3c84c6391b676c528e0682a |
op_doi |
https://doi.org/10.1371/journal.pntd.0002537 |
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PLoS Neglected Tropical Diseases |
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7 |
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11 |
container_start_page |
e2537 |
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