Assessing of the use of proteins A, G, and chimeric protein AG to detect marine mammal immunoglobulins.
In recent years, there has been an increase in infectious diseases in marine mammals, including brucellosis, infections of morbillivirus, herpesvirus, and poxvirus. Several serological diagnostic methods, including enzyme-linked immunosorbent assays, immunofluorescence assays (ELISA), and western bl...
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ftdoajarticles:oai:doaj.org/article:7bf2826bedb54d69a6e0d2908cc28800 2023-10-29T02:35:21+01:00 Assessing of the use of proteins A, G, and chimeric protein AG to detect marine mammal immunoglobulins. Michael Essien Sakyi Takashi Kamio Kaoru Kohyama Md Matiur Rahman Kaori Shimizu Ayaka Okada Yasuo Inoshima 2023-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0291743 https://doaj.org/article/7bf2826bedb54d69a6e0d2908cc28800 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pone.0291743 https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0291743 https://doaj.org/article/7bf2826bedb54d69a6e0d2908cc28800 PLoS ONE, Vol 18, Iss 9, p e0291743 (2023) Medicine R Science Q article 2023 ftdoajarticles https://doi.org/10.1371/journal.pone.0291743 2023-10-01T00:36:07Z In recent years, there has been an increase in infectious diseases in marine mammals, including brucellosis, infections of morbillivirus, herpesvirus, and poxvirus. Several serological diagnostic methods, including enzyme-linked immunosorbent assays, immunofluorescence assays (ELISA), and western blotting, have been used to detect antibodies against pathogens in marine mammals. However, options for commercial secondary antibodies used to detect antibodies in marine mammals are limited; therefore, the use of proteins A, G, or chimeric protein AG may provide a suitable alternative. This study aimed to assess the use of proteins A, G, and chimeric protein AG to detect marine mammal immunoglobulins. Currently, there are no comparative studies on the use of proteins A, G, and chimeric protein AG for the detection of immunoglobulins in marine mammals. In this study, we used ten pinnipeds' species (Baikal seal, California sea lion, harbor seal, northern fur seal, ringed seal, South American fur seal, South American sea lion, spotted seal, Steller sea lion, and walrus) and five cetacean species (beluga whale, bottlenose dolphin, harbor porpoise, killer whale, and Pacific white-sided dolphin) and compare binding ability to proteins A, G, or chimeric protein AG by ELISA. The results revealed that the immunoglobulins from pinniped and cetacean species reacted more strongly to protein A than protein G. In addition, the immunoglobulins of pinnipeds and cetaceans showed a strong binding ability to chimeric protein AG. These results suggest that proteins A, G, and chimeric protein AG would be used to help further develop serological assays. Article in Journal/Newspaper Beluga Beluga whale Beluga* harbor seal Killer Whale ringed seal Killer whale Northern fur seal walrus* Directory of Open Access Journals: DOAJ Articles PLOS ONE 18 9 e0291743 |
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Medicine R Science Q Michael Essien Sakyi Takashi Kamio Kaoru Kohyama Md Matiur Rahman Kaori Shimizu Ayaka Okada Yasuo Inoshima Assessing of the use of proteins A, G, and chimeric protein AG to detect marine mammal immunoglobulins. |
topic_facet |
Medicine R Science Q |
description |
In recent years, there has been an increase in infectious diseases in marine mammals, including brucellosis, infections of morbillivirus, herpesvirus, and poxvirus. Several serological diagnostic methods, including enzyme-linked immunosorbent assays, immunofluorescence assays (ELISA), and western blotting, have been used to detect antibodies against pathogens in marine mammals. However, options for commercial secondary antibodies used to detect antibodies in marine mammals are limited; therefore, the use of proteins A, G, or chimeric protein AG may provide a suitable alternative. This study aimed to assess the use of proteins A, G, and chimeric protein AG to detect marine mammal immunoglobulins. Currently, there are no comparative studies on the use of proteins A, G, and chimeric protein AG for the detection of immunoglobulins in marine mammals. In this study, we used ten pinnipeds' species (Baikal seal, California sea lion, harbor seal, northern fur seal, ringed seal, South American fur seal, South American sea lion, spotted seal, Steller sea lion, and walrus) and five cetacean species (beluga whale, bottlenose dolphin, harbor porpoise, killer whale, and Pacific white-sided dolphin) and compare binding ability to proteins A, G, or chimeric protein AG by ELISA. The results revealed that the immunoglobulins from pinniped and cetacean species reacted more strongly to protein A than protein G. In addition, the immunoglobulins of pinnipeds and cetaceans showed a strong binding ability to chimeric protein AG. These results suggest that proteins A, G, and chimeric protein AG would be used to help further develop serological assays. |
format |
Article in Journal/Newspaper |
author |
Michael Essien Sakyi Takashi Kamio Kaoru Kohyama Md Matiur Rahman Kaori Shimizu Ayaka Okada Yasuo Inoshima |
author_facet |
Michael Essien Sakyi Takashi Kamio Kaoru Kohyama Md Matiur Rahman Kaori Shimizu Ayaka Okada Yasuo Inoshima |
author_sort |
Michael Essien Sakyi |
title |
Assessing of the use of proteins A, G, and chimeric protein AG to detect marine mammal immunoglobulins. |
title_short |
Assessing of the use of proteins A, G, and chimeric protein AG to detect marine mammal immunoglobulins. |
title_full |
Assessing of the use of proteins A, G, and chimeric protein AG to detect marine mammal immunoglobulins. |
title_fullStr |
Assessing of the use of proteins A, G, and chimeric protein AG to detect marine mammal immunoglobulins. |
title_full_unstemmed |
Assessing of the use of proteins A, G, and chimeric protein AG to detect marine mammal immunoglobulins. |
title_sort |
assessing of the use of proteins a, g, and chimeric protein ag to detect marine mammal immunoglobulins. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2023 |
url |
https://doi.org/10.1371/journal.pone.0291743 https://doaj.org/article/7bf2826bedb54d69a6e0d2908cc28800 |
genre |
Beluga Beluga whale Beluga* harbor seal Killer Whale ringed seal Killer whale Northern fur seal walrus* |
genre_facet |
Beluga Beluga whale Beluga* harbor seal Killer Whale ringed seal Killer whale Northern fur seal walrus* |
op_source |
PLoS ONE, Vol 18, Iss 9, p e0291743 (2023) |
op_relation |
https://doi.org/10.1371/journal.pone.0291743 https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0291743 https://doaj.org/article/7bf2826bedb54d69a6e0d2908cc28800 |
op_doi |
https://doi.org/10.1371/journal.pone.0291743 |
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PLOS ONE |
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18 |
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9 |
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e0291743 |
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