Coral calcifying fluid aragonite saturation states derived from Raman spectroscopy
Quantifying the saturation state of aragonite (Ω Ar ) within the calcifying fluid of corals is critical for understanding their biomineralization process and sensitivity to environmental changes including ocean acidification. Recent advances in microscopy, microprobes, and isotope geochemistry enabl...
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ftdoajarticles:oai:doaj.org/article:7ba215bf6f3a480099b89b77102a64f7 2023-05-15T17:50:55+02:00 Coral calcifying fluid aragonite saturation states derived from Raman spectroscopy T. M. DeCarlo J. P. D'Olivo T. Foster M. Holcomb T. Becker M. T. McCulloch 2017-11-01T00:00:00Z https://doi.org/10.5194/bg-14-5253-2017 https://doaj.org/article/7ba215bf6f3a480099b89b77102a64f7 EN eng Copernicus Publications https://www.biogeosciences.net/14/5253/2017/bg-14-5253-2017.pdf https://doaj.org/toc/1726-4170 https://doaj.org/toc/1726-4189 doi:10.5194/bg-14-5253-2017 1726-4170 1726-4189 https://doaj.org/article/7ba215bf6f3a480099b89b77102a64f7 Biogeosciences, Vol 14, Pp 5253-5269 (2017) Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 article 2017 ftdoajarticles https://doi.org/10.5194/bg-14-5253-2017 2022-12-31T02:04:34Z Quantifying the saturation state of aragonite (Ω Ar ) within the calcifying fluid of corals is critical for understanding their biomineralization process and sensitivity to environmental changes including ocean acidification. Recent advances in microscopy, microprobes, and isotope geochemistry enable the determination of calcifying fluid pH and [CO 3 2− ], but direct quantification of Ω Ar (where Ω Ar = [CO 3 2− ][Ca 2+ ]∕ K sp ) has proved elusive. Here we test a new technique for deriving Ω Ar based on Raman spectroscopy. First, we analysed abiogenic aragonite crystals precipitated under a range of Ω Ar from 10 to 34, and we found a strong dependence of Raman peak width on Ω Ar with no significant effects of other factors including pH, Mg∕Ca partitioning, and temperature. Validation of our Raman technique for corals is difficult because there are presently no direct measurements of calcifying fluid Ω Ar available for comparison. However, Raman analysis of the international coral standard JCp-1 produced Ω Ar of 12.3 ± 0.3, which we demonstrate is consistent with published skeletal Mg∕Ca, Sr∕Ca, B∕Ca, δ 11 B, and δ 44 Ca data. Raman measurements are rapid ( ≤ 1 s), high-resolution ( ≤ 1 µm), precise (derived Ω Ar ± 1 to 2 per spectrum depending on instrument configuration), accurate ( ±2 if Ω Ar < 20), and require minimal sample preparation, making the technique well suited for testing the sensitivity of coral calcifying fluid Ω Ar to ocean acidification and warming using samples from natural and laboratory settings. To demonstrate this, we also show a high-resolution time series of Ω Ar over multiple years of growth in a Porites skeleton from the Great Barrier Reef, and we evaluate the response of Ω Ar in juvenile Acropora cultured under elevated CO 2 and temperature. Article in Journal/Newspaper Ocean acidification Directory of Open Access Journals: DOAJ Articles Biogeosciences 14 22 5253 5269 |
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Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 |
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Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 T. M. DeCarlo J. P. D'Olivo T. Foster M. Holcomb T. Becker M. T. McCulloch Coral calcifying fluid aragonite saturation states derived from Raman spectroscopy |
topic_facet |
Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 |
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Quantifying the saturation state of aragonite (Ω Ar ) within the calcifying fluid of corals is critical for understanding their biomineralization process and sensitivity to environmental changes including ocean acidification. Recent advances in microscopy, microprobes, and isotope geochemistry enable the determination of calcifying fluid pH and [CO 3 2− ], but direct quantification of Ω Ar (where Ω Ar = [CO 3 2− ][Ca 2+ ]∕ K sp ) has proved elusive. Here we test a new technique for deriving Ω Ar based on Raman spectroscopy. First, we analysed abiogenic aragonite crystals precipitated under a range of Ω Ar from 10 to 34, and we found a strong dependence of Raman peak width on Ω Ar with no significant effects of other factors including pH, Mg∕Ca partitioning, and temperature. Validation of our Raman technique for corals is difficult because there are presently no direct measurements of calcifying fluid Ω Ar available for comparison. However, Raman analysis of the international coral standard JCp-1 produced Ω Ar of 12.3 ± 0.3, which we demonstrate is consistent with published skeletal Mg∕Ca, Sr∕Ca, B∕Ca, δ 11 B, and δ 44 Ca data. Raman measurements are rapid ( ≤ 1 s), high-resolution ( ≤ 1 µm), precise (derived Ω Ar ± 1 to 2 per spectrum depending on instrument configuration), accurate ( ±2 if Ω Ar < 20), and require minimal sample preparation, making the technique well suited for testing the sensitivity of coral calcifying fluid Ω Ar to ocean acidification and warming using samples from natural and laboratory settings. To demonstrate this, we also show a high-resolution time series of Ω Ar over multiple years of growth in a Porites skeleton from the Great Barrier Reef, and we evaluate the response of Ω Ar in juvenile Acropora cultured under elevated CO 2 and temperature. |
format |
Article in Journal/Newspaper |
author |
T. M. DeCarlo J. P. D'Olivo T. Foster M. Holcomb T. Becker M. T. McCulloch |
author_facet |
T. M. DeCarlo J. P. D'Olivo T. Foster M. Holcomb T. Becker M. T. McCulloch |
author_sort |
T. M. DeCarlo |
title |
Coral calcifying fluid aragonite saturation states derived from Raman spectroscopy |
title_short |
Coral calcifying fluid aragonite saturation states derived from Raman spectroscopy |
title_full |
Coral calcifying fluid aragonite saturation states derived from Raman spectroscopy |
title_fullStr |
Coral calcifying fluid aragonite saturation states derived from Raman spectroscopy |
title_full_unstemmed |
Coral calcifying fluid aragonite saturation states derived from Raman spectroscopy |
title_sort |
coral calcifying fluid aragonite saturation states derived from raman spectroscopy |
publisher |
Copernicus Publications |
publishDate |
2017 |
url |
https://doi.org/10.5194/bg-14-5253-2017 https://doaj.org/article/7ba215bf6f3a480099b89b77102a64f7 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
Biogeosciences, Vol 14, Pp 5253-5269 (2017) |
op_relation |
https://www.biogeosciences.net/14/5253/2017/bg-14-5253-2017.pdf https://doaj.org/toc/1726-4170 https://doaj.org/toc/1726-4189 doi:10.5194/bg-14-5253-2017 1726-4170 1726-4189 https://doaj.org/article/7ba215bf6f3a480099b89b77102a64f7 |
op_doi |
https://doi.org/10.5194/bg-14-5253-2017 |
container_title |
Biogeosciences |
container_volume |
14 |
container_issue |
22 |
container_start_page |
5253 |
op_container_end_page |
5269 |
_version_ |
1766157863774846976 |