Morinda citrifolia Linn. Reduces Parasite Load and Modulates Cytokines and Extracellular Matrix Proteins in C57BL/6 Mice Infected with Leishmania (Leishmania) amazonensis.
The absence of an effective vaccine and the debilitating chemotherapy for Leishmaniasis demonstrate the need for developing alternative treatments. Several studies conducted with Morinda citrifolia have shown various biological activities, including antileishmanial activity, however its mechanisms o...
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ftdoajarticles:oai:doaj.org/article:7b77ed341fc9418994919996953db5b6 2023-05-15T15:13:01+02:00 Morinda citrifolia Linn. Reduces Parasite Load and Modulates Cytokines and Extracellular Matrix Proteins in C57BL/6 Mice Infected with Leishmania (Leishmania) amazonensis. Fernando Almeida-Souza Flávia de Oliveira Cardoso Bruno Vinicius da Conceição Souza Tânia Zaverucha do Valle Joicy Cortez de Sá Iara Dos Santos da Silva Oliveira Celeste da Silva Freitas de Souza Carla Junqueira Moragas Tellis Maria do Socorro Dos Santos Chagas Maria Dutra Behrens Ana Lúcia Abreu-Silva Kátia da Silva Calabrese 2016-08-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0004900 https://doaj.org/article/7b77ed341fc9418994919996953db5b6 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5006983?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004900 https://doaj.org/article/7b77ed341fc9418994919996953db5b6 PLoS Neglected Tropical Diseases, Vol 10, Iss 8, p e0004900 (2016) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2016 ftdoajarticles https://doi.org/10.1371/journal.pntd.0004900 2022-12-31T05:08:07Z The absence of an effective vaccine and the debilitating chemotherapy for Leishmaniasis demonstrate the need for developing alternative treatments. Several studies conducted with Morinda citrifolia have shown various biological activities, including antileishmanial activity, however its mechanisms of action are unknown. This study aimed to analyze the in vivo activity of M. citrifolia fruit juice (Noni) against Leishmania (Leishmania) amazonensis in C57BL/6 mice. M. citrifolia fruit juice from the Brazilian Amazon has shown the same constitution of other juices produced around the world and liquid chromatography-mass spectrometry analysis identified five compounds: deacetylasperulosidic acid, asperulosidic acid, rutin, nonioside B and nonioside C. Daily intragastric treatment with Noni was carried out after 55 days of L. (L.) amazonensis infection in C57BL/6 mice. Parasitic loads, cytokine and extracellular protein matrix expressions of the lesion site were analyzed by qPCR. Histopathology of the lesion site, lymph nodes and liver were performed to evaluate the inflammatory processes. Cytokines and biochemical parameters of toxicity from sera were also evaluated. The Noni treatment at 500 mg.kg-1.day-1 for 60 days decreased the lesion size and parasitic load in the footpad infected with L. (L.) amazonensis. The site of infection also showed decreased inflammatory infiltrates and decreased cytokine expressions for IL-12, TNF-α, TGF-β and IL-10. On the other hand, Noni treatment enhanced the extracellular matrix protein expressions of collagen IV, fibronectin and laminin in the infected footpad as well collagen I and II, fibronectin and laminin in the mock-infected footpads. No toxicity was observed at the end of treatment. These data show the efficacy of Noni treatment. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 10 8 e0004900 |
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Directory of Open Access Journals: DOAJ Articles |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Fernando Almeida-Souza Flávia de Oliveira Cardoso Bruno Vinicius da Conceição Souza Tânia Zaverucha do Valle Joicy Cortez de Sá Iara Dos Santos da Silva Oliveira Celeste da Silva Freitas de Souza Carla Junqueira Moragas Tellis Maria do Socorro Dos Santos Chagas Maria Dutra Behrens Ana Lúcia Abreu-Silva Kátia da Silva Calabrese Morinda citrifolia Linn. Reduces Parasite Load and Modulates Cytokines and Extracellular Matrix Proteins in C57BL/6 Mice Infected with Leishmania (Leishmania) amazonensis. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
The absence of an effective vaccine and the debilitating chemotherapy for Leishmaniasis demonstrate the need for developing alternative treatments. Several studies conducted with Morinda citrifolia have shown various biological activities, including antileishmanial activity, however its mechanisms of action are unknown. This study aimed to analyze the in vivo activity of M. citrifolia fruit juice (Noni) against Leishmania (Leishmania) amazonensis in C57BL/6 mice. M. citrifolia fruit juice from the Brazilian Amazon has shown the same constitution of other juices produced around the world and liquid chromatography-mass spectrometry analysis identified five compounds: deacetylasperulosidic acid, asperulosidic acid, rutin, nonioside B and nonioside C. Daily intragastric treatment with Noni was carried out after 55 days of L. (L.) amazonensis infection in C57BL/6 mice. Parasitic loads, cytokine and extracellular protein matrix expressions of the lesion site were analyzed by qPCR. Histopathology of the lesion site, lymph nodes and liver were performed to evaluate the inflammatory processes. Cytokines and biochemical parameters of toxicity from sera were also evaluated. The Noni treatment at 500 mg.kg-1.day-1 for 60 days decreased the lesion size and parasitic load in the footpad infected with L. (L.) amazonensis. The site of infection also showed decreased inflammatory infiltrates and decreased cytokine expressions for IL-12, TNF-α, TGF-β and IL-10. On the other hand, Noni treatment enhanced the extracellular matrix protein expressions of collagen IV, fibronectin and laminin in the infected footpad as well collagen I and II, fibronectin and laminin in the mock-infected footpads. No toxicity was observed at the end of treatment. These data show the efficacy of Noni treatment. |
format |
Article in Journal/Newspaper |
author |
Fernando Almeida-Souza Flávia de Oliveira Cardoso Bruno Vinicius da Conceição Souza Tânia Zaverucha do Valle Joicy Cortez de Sá Iara Dos Santos da Silva Oliveira Celeste da Silva Freitas de Souza Carla Junqueira Moragas Tellis Maria do Socorro Dos Santos Chagas Maria Dutra Behrens Ana Lúcia Abreu-Silva Kátia da Silva Calabrese |
author_facet |
Fernando Almeida-Souza Flávia de Oliveira Cardoso Bruno Vinicius da Conceição Souza Tânia Zaverucha do Valle Joicy Cortez de Sá Iara Dos Santos da Silva Oliveira Celeste da Silva Freitas de Souza Carla Junqueira Moragas Tellis Maria do Socorro Dos Santos Chagas Maria Dutra Behrens Ana Lúcia Abreu-Silva Kátia da Silva Calabrese |
author_sort |
Fernando Almeida-Souza |
title |
Morinda citrifolia Linn. Reduces Parasite Load and Modulates Cytokines and Extracellular Matrix Proteins in C57BL/6 Mice Infected with Leishmania (Leishmania) amazonensis. |
title_short |
Morinda citrifolia Linn. Reduces Parasite Load and Modulates Cytokines and Extracellular Matrix Proteins in C57BL/6 Mice Infected with Leishmania (Leishmania) amazonensis. |
title_full |
Morinda citrifolia Linn. Reduces Parasite Load and Modulates Cytokines and Extracellular Matrix Proteins in C57BL/6 Mice Infected with Leishmania (Leishmania) amazonensis. |
title_fullStr |
Morinda citrifolia Linn. Reduces Parasite Load and Modulates Cytokines and Extracellular Matrix Proteins in C57BL/6 Mice Infected with Leishmania (Leishmania) amazonensis. |
title_full_unstemmed |
Morinda citrifolia Linn. Reduces Parasite Load and Modulates Cytokines and Extracellular Matrix Proteins in C57BL/6 Mice Infected with Leishmania (Leishmania) amazonensis. |
title_sort |
morinda citrifolia linn. reduces parasite load and modulates cytokines and extracellular matrix proteins in c57bl/6 mice infected with leishmania (leishmania) amazonensis. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2016 |
url |
https://doi.org/10.1371/journal.pntd.0004900 https://doaj.org/article/7b77ed341fc9418994919996953db5b6 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 10, Iss 8, p e0004900 (2016) |
op_relation |
http://europepmc.org/articles/PMC5006983?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004900 https://doaj.org/article/7b77ed341fc9418994919996953db5b6 |
op_doi |
https://doi.org/10.1371/journal.pntd.0004900 |
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PLOS Neglected Tropical Diseases |
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e0004900 |
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