Dried-blood spots: a cost-effective field method for the detection of Chikungunya virus circulation in remote areas.
BACKGROUND: In 2005, there were outbreaks of febrile polyarthritis due to Chikungunya virus (CHIKV) in the Comoros Islands. CHIKV then spread to other islands in the Indian Ocean: La Réunion, Mauritius, Seychelles and Madagascar. These outbreaks revealed the lack of surveillance and preparedness of...
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ftdoajarticles:oai:doaj.org/article:7b76bd88fc434eeab04a86fdc836f1ee 2023-05-15T15:13:54+02:00 Dried-blood spots: a cost-effective field method for the detection of Chikungunya virus circulation in remote areas. Soa Fy Andriamandimby Jean-Michel Heraud Laurence Randrianasolo Jean Théophile Rafisandratantsoa Seta Andriamamonjy Vincent Richard 2013-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0002339 https://doaj.org/article/7b76bd88fc434eeab04a86fdc836f1ee EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3723542?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002339 https://doaj.org/article/7b76bd88fc434eeab04a86fdc836f1ee PLoS Neglected Tropical Diseases, Vol 7, Iss 7, p e2339 (2013) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2013 ftdoajarticles https://doi.org/10.1371/journal.pntd.0002339 2022-12-30T21:57:31Z BACKGROUND: In 2005, there were outbreaks of febrile polyarthritis due to Chikungunya virus (CHIKV) in the Comoros Islands. CHIKV then spread to other islands in the Indian Ocean: La Réunion, Mauritius, Seychelles and Madagascar. These outbreaks revealed the lack of surveillance and preparedness of Madagascar and other countries. Thus, it was decided in 2007 to establish a syndrome-based surveillance network to monitor dengue-like illness. OBJECTIVE: This study aims to evaluate the use of capillary blood samples blotted on filter papers for molecular diagnosis of CHIKV infection. Venous blood samples can be difficult to obtain and the shipment of serum in appropriate temperature conditions is too costly for most developing countries. METHODOLOGY AND PRINCIPAL FINDINGS: Venous blood and dried-blood blotted on filter paper (DBFP) were collected during the last CHIKV outbreak in Madagascar (2010) and as part of our routine surveillance of dengue-like illness. All samples were tested by real-time RT-PCR and results with serum and DBFP samples were compared for each patient. The sensitivity and specificity of tests performed with DBFP, relative to those with venous samples (defined as 100%) were 93.1% (95% CI:[84.7-97.7]) and 94.4% (95% CI:[88.3-97.7]), respectively. The Kappa coefficient 0.87 (95% CI:[0.80-0.94]) was excellent. CONCLUSION: This study shows that DBFP specimens can be used as a cost-effective alternative sampling method for the surveillance and monitoring of CHIKV circulation and emergence in developing countries, and probably also for other arboviruses. The loss of sensitivity is insignificant and involved a very small number of patients, all with low viral loads. Whether viruses can be isolated from dried blood spots remains to be determined. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Indian PLoS Neglected Tropical Diseases 7 7 e2339 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Soa Fy Andriamandimby Jean-Michel Heraud Laurence Randrianasolo Jean Théophile Rafisandratantsoa Seta Andriamamonjy Vincent Richard Dried-blood spots: a cost-effective field method for the detection of Chikungunya virus circulation in remote areas. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND: In 2005, there were outbreaks of febrile polyarthritis due to Chikungunya virus (CHIKV) in the Comoros Islands. CHIKV then spread to other islands in the Indian Ocean: La Réunion, Mauritius, Seychelles and Madagascar. These outbreaks revealed the lack of surveillance and preparedness of Madagascar and other countries. Thus, it was decided in 2007 to establish a syndrome-based surveillance network to monitor dengue-like illness. OBJECTIVE: This study aims to evaluate the use of capillary blood samples blotted on filter papers for molecular diagnosis of CHIKV infection. Venous blood samples can be difficult to obtain and the shipment of serum in appropriate temperature conditions is too costly for most developing countries. METHODOLOGY AND PRINCIPAL FINDINGS: Venous blood and dried-blood blotted on filter paper (DBFP) were collected during the last CHIKV outbreak in Madagascar (2010) and as part of our routine surveillance of dengue-like illness. All samples were tested by real-time RT-PCR and results with serum and DBFP samples were compared for each patient. The sensitivity and specificity of tests performed with DBFP, relative to those with venous samples (defined as 100%) were 93.1% (95% CI:[84.7-97.7]) and 94.4% (95% CI:[88.3-97.7]), respectively. The Kappa coefficient 0.87 (95% CI:[0.80-0.94]) was excellent. CONCLUSION: This study shows that DBFP specimens can be used as a cost-effective alternative sampling method for the surveillance and monitoring of CHIKV circulation and emergence in developing countries, and probably also for other arboviruses. The loss of sensitivity is insignificant and involved a very small number of patients, all with low viral loads. Whether viruses can be isolated from dried blood spots remains to be determined. |
format |
Article in Journal/Newspaper |
author |
Soa Fy Andriamandimby Jean-Michel Heraud Laurence Randrianasolo Jean Théophile Rafisandratantsoa Seta Andriamamonjy Vincent Richard |
author_facet |
Soa Fy Andriamandimby Jean-Michel Heraud Laurence Randrianasolo Jean Théophile Rafisandratantsoa Seta Andriamamonjy Vincent Richard |
author_sort |
Soa Fy Andriamandimby |
title |
Dried-blood spots: a cost-effective field method for the detection of Chikungunya virus circulation in remote areas. |
title_short |
Dried-blood spots: a cost-effective field method for the detection of Chikungunya virus circulation in remote areas. |
title_full |
Dried-blood spots: a cost-effective field method for the detection of Chikungunya virus circulation in remote areas. |
title_fullStr |
Dried-blood spots: a cost-effective field method for the detection of Chikungunya virus circulation in remote areas. |
title_full_unstemmed |
Dried-blood spots: a cost-effective field method for the detection of Chikungunya virus circulation in remote areas. |
title_sort |
dried-blood spots: a cost-effective field method for the detection of chikungunya virus circulation in remote areas. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2013 |
url |
https://doi.org/10.1371/journal.pntd.0002339 https://doaj.org/article/7b76bd88fc434eeab04a86fdc836f1ee |
geographic |
Arctic Indian |
geographic_facet |
Arctic Indian |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 7, Iss 7, p e2339 (2013) |
op_relation |
http://europepmc.org/articles/PMC3723542?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002339 https://doaj.org/article/7b76bd88fc434eeab04a86fdc836f1ee |
op_doi |
https://doi.org/10.1371/journal.pntd.0002339 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
7 |
container_issue |
7 |
container_start_page |
e2339 |
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1766344415084806144 |