Comparative Proteomics Identifies Host Immune System Proteins Affected by Infection with Mycobacterium bovis.
Mycobacteria of the Mycobacterium tuberculosis complex (MTBC) greatly impact human and animal health worldwide. The mycobacterial life cycle is complex, and the mechanisms resulting in pathogen infection and survival in host cells are not fully understood. Eurasian wild boar (Sus scrofa) are natural...
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ftdoajarticles:oai:doaj.org/article:7b5e831bdea64fdbbfd56b80ea798500 2023-05-15T15:14:30+02:00 Comparative Proteomics Identifies Host Immune System Proteins Affected by Infection with Mycobacterium bovis. Vladimir López Margarita Villar João Queirós Joaquín Vicente Lourdes Mateos-Hernández Iratxe Díez-Delgado Marinela Contreras Paulo C Alves Pilar Alberdi Christian Gortázar José de la Fuente 2016-03-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0004541 https://doaj.org/article/7b5e831bdea64fdbbfd56b80ea798500 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4814110?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004541 https://doaj.org/article/7b5e831bdea64fdbbfd56b80ea798500 PLoS Neglected Tropical Diseases, Vol 10, Iss 3, p e0004541 (2016) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2016 ftdoajarticles https://doi.org/10.1371/journal.pntd.0004541 2022-12-31T11:55:29Z Mycobacteria of the Mycobacterium tuberculosis complex (MTBC) greatly impact human and animal health worldwide. The mycobacterial life cycle is complex, and the mechanisms resulting in pathogen infection and survival in host cells are not fully understood. Eurasian wild boar (Sus scrofa) are natural reservoir hosts for MTBC and a model for mycobacterial infection and tuberculosis (TB). In the wild boar TB model, mycobacterial infection affects the expression of innate and adaptive immune response genes in mandibular lymph nodes and oropharyngeal tonsils, and biomarkers have been proposed as correlates with resistance to natural infection. However, the mechanisms used by mycobacteria to manipulate host immune response are not fully characterized. Our hypothesis is that the immune system proteins under-represented in infected animals, when compared to uninfected controls, are used by mycobacteria to guarantee pathogen infection and transmission. To address this hypothesis, a comparative proteomics approach was used to compare host response between uninfected (TB-) and M. bovis-infected young (TB+) and adult animals with different infection status [TB lesions localized in the head (TB+) or affecting multiple organs (TB++)]. The results identified host immune system proteins that play an important role in host response to mycobacteria. Calcium binding protein A9, Heme peroxidase, Lactotransferrin, Cathelicidin and Peptidoglycan-recognition protein were under-represented in TB+ animals when compared to uninfected TB- controls, but protein levels were higher as infection progressed in TB++ animals when compared to TB- and/or TB+ adult wild boar. MHCI was the only protein over-represented in TB+ adult wild boar when compared to uninfected TB- controls. The results reported here suggest that M. bovis manipulates host immune response by reducing the production of immune system proteins. However, as infection progresses, wild boar immune response recovers to limit pathogen multiplication and promote survival, facilitating ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 10 3 e0004541 |
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language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Vladimir López Margarita Villar João Queirós Joaquín Vicente Lourdes Mateos-Hernández Iratxe Díez-Delgado Marinela Contreras Paulo C Alves Pilar Alberdi Christian Gortázar José de la Fuente Comparative Proteomics Identifies Host Immune System Proteins Affected by Infection with Mycobacterium bovis. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Mycobacteria of the Mycobacterium tuberculosis complex (MTBC) greatly impact human and animal health worldwide. The mycobacterial life cycle is complex, and the mechanisms resulting in pathogen infection and survival in host cells are not fully understood. Eurasian wild boar (Sus scrofa) are natural reservoir hosts for MTBC and a model for mycobacterial infection and tuberculosis (TB). In the wild boar TB model, mycobacterial infection affects the expression of innate and adaptive immune response genes in mandibular lymph nodes and oropharyngeal tonsils, and biomarkers have been proposed as correlates with resistance to natural infection. However, the mechanisms used by mycobacteria to manipulate host immune response are not fully characterized. Our hypothesis is that the immune system proteins under-represented in infected animals, when compared to uninfected controls, are used by mycobacteria to guarantee pathogen infection and transmission. To address this hypothesis, a comparative proteomics approach was used to compare host response between uninfected (TB-) and M. bovis-infected young (TB+) and adult animals with different infection status [TB lesions localized in the head (TB+) or affecting multiple organs (TB++)]. The results identified host immune system proteins that play an important role in host response to mycobacteria. Calcium binding protein A9, Heme peroxidase, Lactotransferrin, Cathelicidin and Peptidoglycan-recognition protein were under-represented in TB+ animals when compared to uninfected TB- controls, but protein levels were higher as infection progressed in TB++ animals when compared to TB- and/or TB+ adult wild boar. MHCI was the only protein over-represented in TB+ adult wild boar when compared to uninfected TB- controls. The results reported here suggest that M. bovis manipulates host immune response by reducing the production of immune system proteins. However, as infection progresses, wild boar immune response recovers to limit pathogen multiplication and promote survival, facilitating ... |
format |
Article in Journal/Newspaper |
author |
Vladimir López Margarita Villar João Queirós Joaquín Vicente Lourdes Mateos-Hernández Iratxe Díez-Delgado Marinela Contreras Paulo C Alves Pilar Alberdi Christian Gortázar José de la Fuente |
author_facet |
Vladimir López Margarita Villar João Queirós Joaquín Vicente Lourdes Mateos-Hernández Iratxe Díez-Delgado Marinela Contreras Paulo C Alves Pilar Alberdi Christian Gortázar José de la Fuente |
author_sort |
Vladimir López |
title |
Comparative Proteomics Identifies Host Immune System Proteins Affected by Infection with Mycobacterium bovis. |
title_short |
Comparative Proteomics Identifies Host Immune System Proteins Affected by Infection with Mycobacterium bovis. |
title_full |
Comparative Proteomics Identifies Host Immune System Proteins Affected by Infection with Mycobacterium bovis. |
title_fullStr |
Comparative Proteomics Identifies Host Immune System Proteins Affected by Infection with Mycobacterium bovis. |
title_full_unstemmed |
Comparative Proteomics Identifies Host Immune System Proteins Affected by Infection with Mycobacterium bovis. |
title_sort |
comparative proteomics identifies host immune system proteins affected by infection with mycobacterium bovis. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2016 |
url |
https://doi.org/10.1371/journal.pntd.0004541 https://doaj.org/article/7b5e831bdea64fdbbfd56b80ea798500 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 10, Iss 3, p e0004541 (2016) |
op_relation |
http://europepmc.org/articles/PMC4814110?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004541 https://doaj.org/article/7b5e831bdea64fdbbfd56b80ea798500 |
op_doi |
https://doi.org/10.1371/journal.pntd.0004541 |
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PLOS Neglected Tropical Diseases |
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10 |
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3 |
container_start_page |
e0004541 |
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1766344947249709056 |