The recovery and protective effects of asiatic acid on differentiated human neuroblastoma SH-SY5Y cells cytotoxic-induced by cholesterol

• Published in: Asian Pacific Journal of Tropical Biomedicine
• Main Authors: Kanchanat Ternchoocheep, Damrassamon Surangkul, Sukhgij Ysothonsreekul
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wolters Kluwer Medknow Publications 2017
• Subjects: Asiatic acid; Cholesterol; SH-SY5Y cells; Cell viability; Arctic medicine. Tropical medicine; RC955-962; Biology (General); QH301-705.5; Arctic
• Online Access: https://doi.org/10.1016/j.apjtb.2017.01.012; https://doaj.org/article/7b4e6c49b35646ce8a418ca4d48f7488

Objective: To investigate the effect of asiatic acid (AA) on the differentiated human neuroblastoma SH-SY5Y cells cytotoxic-induced by cholesterol. Methods: Human neuroblastoma SH-SY5Y cells were either exposed to different concentrations of AA or treated with different doses of cholesterol to reveal their responding viability by MTT assay. The selective 1 μmol/L concentration of AA was then used to test for either the protective or the recovery effects on the cells treated with 250 μmol/L concentration of cholesterol. Results: AA has a propensity to directly increase the viability of differentiated human neuroblastoma SH-SY5Y cells. Cholesterol has significant cytotoxic effect on those cells in a concentration-dependent manner. AA has the ability to slightly recover the viability of the differentiated culture cytotoxic-induced by cholesterol but could not protect those cells from cytotoxic-induced by cholesterol. Conclusions: High concentrations of cholesterol were observed to be harmful to the neurons and AA had a slight effect of reducing neuronal death caused by cholesterol.