Bone marrow chimeric mice reveal a dual role for CD36 in Plasmodium berghei ANKA infection

Abstract Background Adhesion of Plasmodium -infected red blood cells (iRBC) to different host cells, ranging from endothelial to red blood cells, is associated to malaria pathology. In vitro studies have shown the relevance of CD36 for adhesion phenotypes of Plasmodium falciparum iRBC such as seques...

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Published in:Malaria Journal
Main Authors: Febbraio Maria, Portugal Sílvia, Cunha-Rodrigues Margarida, Mota Maria M
Format: Article in Journal/Newspaper
Language:English
Published: BMC 2007
Subjects:
Online Access:https://doi.org/10.1186/1475-2875-6-32
https://doaj.org/article/7b12c921cc8d47c2b1b480c9b60b3f01
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spelling ftdoajarticles:oai:doaj.org/article:7b12c921cc8d47c2b1b480c9b60b3f01 2023-05-15T15:15:11+02:00 Bone marrow chimeric mice reveal a dual role for CD36 in Plasmodium berghei ANKA infection Febbraio Maria Portugal Sílvia Cunha-Rodrigues Margarida Mota Maria M 2007-03-01T00:00:00Z https://doi.org/10.1186/1475-2875-6-32 https://doaj.org/article/7b12c921cc8d47c2b1b480c9b60b3f01 EN eng BMC http://www.malariajournal.com/content/6/1/32 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-6-32 1475-2875 https://doaj.org/article/7b12c921cc8d47c2b1b480c9b60b3f01 Malaria Journal, Vol 6, Iss 1, p 32 (2007) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2007 ftdoajarticles https://doi.org/10.1186/1475-2875-6-32 2022-12-31T08:08:48Z Abstract Background Adhesion of Plasmodium -infected red blood cells (iRBC) to different host cells, ranging from endothelial to red blood cells, is associated to malaria pathology. In vitro studies have shown the relevance of CD36 for adhesion phenotypes of Plasmodium falciparum iRBC such as sequestration, platelet mediated clumping and non-opsonic uptake of iRBC. Different adhesion phenotypes involve different host cells and are associated with different pathological outcomes of disease. Studies with different human populations with CD36 polymorphisms failed to attribute a clear role to CD36 expression in human malaria. Up to the present, no in vivo model has been available to study the relevance of different CD36 adhesion phenotypes to the pathological course of Plasmodium infection. Methods Using CD36-deficient mice and their control littermates, CD36 bone marrow chimeric mice, expressing CD36 exclusively in haematopoietic cells or in non-haematopoietic cells, were generated. Irradiated CD36 -/- and wild type mice were also reconstituted with syngeneic cells to control for the effects of irradiation. The reconstituted mice were infected with Plasmodium berghei ANKA and analysed for the development of blood parasitaemia and neurological symptoms. Results All mice reconstituted with syngeneic bone marrow cells as well as chimeric mice expressing CD36 exclusively in non-haematopoietic cells died from experimental cerebral malaria between day 6 and 12 after infection. A significant proportion of chimeric mice expressing CD36 only in haematopoietic cells did not die from cerebral malaria. Conclusion The analysis of bone marrow chimeric mice reveals a dual role of CD36 in P. berghei ANKA infection. Expression of CD36 in haematopoietic cells, most likely macrophages and dendritic cells, has a beneficial effect that is masked in normal mice by adverse effects of CD36 expression in non-haematopoietic cells, most likely endothelial cells. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 6 1 32
institution Open Polar
collection Directory of Open Access Journals: DOAJ Articles
op_collection_id ftdoajarticles
language English
topic Arctic medicine. Tropical medicine
RC955-962
Infectious and parasitic diseases
RC109-216
spellingShingle Arctic medicine. Tropical medicine
RC955-962
Infectious and parasitic diseases
RC109-216
Febbraio Maria
Portugal Sílvia
Cunha-Rodrigues Margarida
Mota Maria M
Bone marrow chimeric mice reveal a dual role for CD36 in Plasmodium berghei ANKA infection
topic_facet Arctic medicine. Tropical medicine
RC955-962
Infectious and parasitic diseases
RC109-216
description Abstract Background Adhesion of Plasmodium -infected red blood cells (iRBC) to different host cells, ranging from endothelial to red blood cells, is associated to malaria pathology. In vitro studies have shown the relevance of CD36 for adhesion phenotypes of Plasmodium falciparum iRBC such as sequestration, platelet mediated clumping and non-opsonic uptake of iRBC. Different adhesion phenotypes involve different host cells and are associated with different pathological outcomes of disease. Studies with different human populations with CD36 polymorphisms failed to attribute a clear role to CD36 expression in human malaria. Up to the present, no in vivo model has been available to study the relevance of different CD36 adhesion phenotypes to the pathological course of Plasmodium infection. Methods Using CD36-deficient mice and their control littermates, CD36 bone marrow chimeric mice, expressing CD36 exclusively in haematopoietic cells or in non-haematopoietic cells, were generated. Irradiated CD36 -/- and wild type mice were also reconstituted with syngeneic cells to control for the effects of irradiation. The reconstituted mice were infected with Plasmodium berghei ANKA and analysed for the development of blood parasitaemia and neurological symptoms. Results All mice reconstituted with syngeneic bone marrow cells as well as chimeric mice expressing CD36 exclusively in non-haematopoietic cells died from experimental cerebral malaria between day 6 and 12 after infection. A significant proportion of chimeric mice expressing CD36 only in haematopoietic cells did not die from cerebral malaria. Conclusion The analysis of bone marrow chimeric mice reveals a dual role of CD36 in P. berghei ANKA infection. Expression of CD36 in haematopoietic cells, most likely macrophages and dendritic cells, has a beneficial effect that is masked in normal mice by adverse effects of CD36 expression in non-haematopoietic cells, most likely endothelial cells.
format Article in Journal/Newspaper
author Febbraio Maria
Portugal Sílvia
Cunha-Rodrigues Margarida
Mota Maria M
author_facet Febbraio Maria
Portugal Sílvia
Cunha-Rodrigues Margarida
Mota Maria M
author_sort Febbraio Maria
title Bone marrow chimeric mice reveal a dual role for CD36 in Plasmodium berghei ANKA infection
title_short Bone marrow chimeric mice reveal a dual role for CD36 in Plasmodium berghei ANKA infection
title_full Bone marrow chimeric mice reveal a dual role for CD36 in Plasmodium berghei ANKA infection
title_fullStr Bone marrow chimeric mice reveal a dual role for CD36 in Plasmodium berghei ANKA infection
title_full_unstemmed Bone marrow chimeric mice reveal a dual role for CD36 in Plasmodium berghei ANKA infection
title_sort bone marrow chimeric mice reveal a dual role for cd36 in plasmodium berghei anka infection
publisher BMC
publishDate 2007
url https://doi.org/10.1186/1475-2875-6-32
https://doaj.org/article/7b12c921cc8d47c2b1b480c9b60b3f01
geographic Arctic
geographic_facet Arctic
genre Arctic
genre_facet Arctic
op_source Malaria Journal, Vol 6, Iss 1, p 32 (2007)
op_relation http://www.malariajournal.com/content/6/1/32
https://doaj.org/toc/1475-2875
doi:10.1186/1475-2875-6-32
1475-2875
https://doaj.org/article/7b12c921cc8d47c2b1b480c9b60b3f01
op_doi https://doi.org/10.1186/1475-2875-6-32
container_title Malaria Journal
container_volume 6
container_issue 1
container_start_page 32
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