Production and characterization of monoclonal antibodies to the edta extract of Leptospira interrogans, serovar icterohaemorrhagiae
Monoclonal antibodies (MABs) ivere produced against an etbylenediaminetetraacetate (EDTA) extract of Leptospira interrogans serovar icterohaemorrhagiae being characterized by gel precipitation as IgM and IgG (IgGl and IgG2b). The EDTA extract was detected as several bands by silver staining in SDS-P...
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Sociedade Brasileira de Medicina Tropical (SBMT)
1996
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ftdoajarticles:oai:doaj.org/article:7ae9632ca7ad4f5da40c58c78965d740 2023-05-15T15:10:37+02:00 Production and characterization of monoclonal antibodies to the edta extract of Leptospira interrogans, serovar icterohaemorrhagiae Lilian Terezinha de Queiroz Leite Mauricio Resende Wanderley de Souza Elizabeth R.S. Camargos Matilde Cota Koury 1996-10-01T00:00:00Z https://doaj.org/article/7ae9632ca7ad4f5da40c58c78965d740 EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86821996000500012 https://doaj.org/toc/0037-8682 https://doaj.org/toc/1678-9849 0037-8682 1678-9849 https://doaj.org/article/7ae9632ca7ad4f5da40c58c78965d740 Revista da Sociedade Brasileira de Medicina Tropical, Vol 29, Iss 5, Pp 483-489 (1996) Leptospira Anticorpos monoclonais Proteção passiva Susceptibility Monoclonal antibodies Passive protection Arctic medicine. Tropical medicine RC955-962 article 1996 ftdoajarticles 2022-12-31T02:52:58Z Monoclonal antibodies (MABs) ivere produced against an etbylenediaminetetraacetate (EDTA) extract of Leptospira interrogans serovar icterohaemorrhagiae being characterized by gel precipitation as IgM and IgG (IgGl and IgG2b). The EDTA extract was detected as several bands by silver staining in SDS-PAGE. In the Western blot the bands around 20 KDa reacted with a monoclonal antibody, 47B4D6, and was oxidized by periodate and was not digested by pronase, suggesting that the determinant is of carbohydrate nature, lmmunocytochemistry, using colloidal gold labeling, showed that an EDTA extract determinant recognized by monoclonal antibody 47B4D6, is localized under the outer envelope of serovar icterohaemorrhagiae. Hoe AIAB raised against the EDTA extract was not able to protect hamsters from lethal challenge with virulent homologous leptospires. Anticorpos monoclonais (AcM) foram produzidos contra o extrato EDTA obtido de Leptospira interrogans, sorovar icterohaemorrhagiae. Pelo teste de precipitação foram caracterizados como IgM e IgG (IgGl e IgG2). A eletroforese em gel de poliacrilamida do extrato EDTA revelou diversas bandas quando corada pela prata. No "Western blot", as bandas em torno de 20 kDa reagiram com o AcM 47B4D6, foram oxidadas pelo periodato e não digeridas pela pronase, sugerindo que o determinante é de natureza carboidrato. O determinante reconhecido pelo AcM 47B4D6 estã localizado sob o envelope externo como revelado pela imunocitoquímica usando marcação com ouro coloidal. O AcM contra extrato EDTA do sorovar icterohaemorrahagiae não protegeu hamsters quando inoculados com lepstopira homóloga virulenta. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Sob’ ENVELOPE(66.156,66.156,66.322,66.322) |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Leptospira Anticorpos monoclonais Proteção passiva Susceptibility Monoclonal antibodies Passive protection Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
Leptospira Anticorpos monoclonais Proteção passiva Susceptibility Monoclonal antibodies Passive protection Arctic medicine. Tropical medicine RC955-962 Lilian Terezinha de Queiroz Leite Mauricio Resende Wanderley de Souza Elizabeth R.S. Camargos Matilde Cota Koury Production and characterization of monoclonal antibodies to the edta extract of Leptospira interrogans, serovar icterohaemorrhagiae |
topic_facet |
Leptospira Anticorpos monoclonais Proteção passiva Susceptibility Monoclonal antibodies Passive protection Arctic medicine. Tropical medicine RC955-962 |
description |
Monoclonal antibodies (MABs) ivere produced against an etbylenediaminetetraacetate (EDTA) extract of Leptospira interrogans serovar icterohaemorrhagiae being characterized by gel precipitation as IgM and IgG (IgGl and IgG2b). The EDTA extract was detected as several bands by silver staining in SDS-PAGE. In the Western blot the bands around 20 KDa reacted with a monoclonal antibody, 47B4D6, and was oxidized by periodate and was not digested by pronase, suggesting that the determinant is of carbohydrate nature, lmmunocytochemistry, using colloidal gold labeling, showed that an EDTA extract determinant recognized by monoclonal antibody 47B4D6, is localized under the outer envelope of serovar icterohaemorrhagiae. Hoe AIAB raised against the EDTA extract was not able to protect hamsters from lethal challenge with virulent homologous leptospires. Anticorpos monoclonais (AcM) foram produzidos contra o extrato EDTA obtido de Leptospira interrogans, sorovar icterohaemorrhagiae. Pelo teste de precipitação foram caracterizados como IgM e IgG (IgGl e IgG2). A eletroforese em gel de poliacrilamida do extrato EDTA revelou diversas bandas quando corada pela prata. No "Western blot", as bandas em torno de 20 kDa reagiram com o AcM 47B4D6, foram oxidadas pelo periodato e não digeridas pela pronase, sugerindo que o determinante é de natureza carboidrato. O determinante reconhecido pelo AcM 47B4D6 estã localizado sob o envelope externo como revelado pela imunocitoquímica usando marcação com ouro coloidal. O AcM contra extrato EDTA do sorovar icterohaemorrahagiae não protegeu hamsters quando inoculados com lepstopira homóloga virulenta. |
format |
Article in Journal/Newspaper |
author |
Lilian Terezinha de Queiroz Leite Mauricio Resende Wanderley de Souza Elizabeth R.S. Camargos Matilde Cota Koury |
author_facet |
Lilian Terezinha de Queiroz Leite Mauricio Resende Wanderley de Souza Elizabeth R.S. Camargos Matilde Cota Koury |
author_sort |
Lilian Terezinha de Queiroz Leite |
title |
Production and characterization of monoclonal antibodies to the edta extract of Leptospira interrogans, serovar icterohaemorrhagiae |
title_short |
Production and characterization of monoclonal antibodies to the edta extract of Leptospira interrogans, serovar icterohaemorrhagiae |
title_full |
Production and characterization of monoclonal antibodies to the edta extract of Leptospira interrogans, serovar icterohaemorrhagiae |
title_fullStr |
Production and characterization of monoclonal antibodies to the edta extract of Leptospira interrogans, serovar icterohaemorrhagiae |
title_full_unstemmed |
Production and characterization of monoclonal antibodies to the edta extract of Leptospira interrogans, serovar icterohaemorrhagiae |
title_sort |
production and characterization of monoclonal antibodies to the edta extract of leptospira interrogans, serovar icterohaemorrhagiae |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
1996 |
url |
https://doaj.org/article/7ae9632ca7ad4f5da40c58c78965d740 |
long_lat |
ENVELOPE(66.156,66.156,66.322,66.322) |
geographic |
Arctic Sob’ |
geographic_facet |
Arctic Sob’ |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 29, Iss 5, Pp 483-489 (1996) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86821996000500012 https://doaj.org/toc/0037-8682 https://doaj.org/toc/1678-9849 0037-8682 1678-9849 https://doaj.org/article/7ae9632ca7ad4f5da40c58c78965d740 |
_version_ |
1766341615081750528 |