Antimicrobial resistance patterns and prevalence of integrons in Shigella species isolated from children with diarrhea in southwest Iran
Objective: To investigate the antimicrobial resistance patterns and prevalence of integrons in Shigella species isolated from children with diarrhea in southwest Iran. Methods: In this study, 1 530 stool samples were collected from children under 15 years with diarrhea referred to teaching hospitals...
Published in: | Asian Pacific Journal of Tropical Medicine |
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ftdoajarticles:oai:doaj.org/article:7ade4721edd8441a8374018d496ec095 2023-05-15T15:07:50+02:00 Antimicrobial resistance patterns and prevalence of integrons in Shigella species isolated from children with diarrhea in southwest Iran Nabi Jomehzadeh Maryam Afzali Khadijeh Ahmadi Shokrollah Salmanzadeh Fateme Jahangiri Mehr 2021-01-01T00:00:00Z https://doi.org/10.4103/1995-7645.281529 https://doaj.org/article/7ade4721edd8441a8374018d496ec095 EN eng Wolters Kluwer Medknow Publications http://www.apjtm.org/article.asp?issn=1995-7645;year=2021;volume=14;issue=2;spage=78;epage=82;aulast=Jomehzadeh https://doaj.org/toc/2352-4146 2352-4146 doi:10.4103/1995-7645.281529 https://doaj.org/article/7ade4721edd8441a8374018d496ec095 Asian Pacific Journal of Tropical Medicine, Vol 14, Iss 2, Pp 78-82 (2021) integrons shigella spp. multi-drug resistance pcr Arctic medicine. Tropical medicine RC955-962 article 2021 ftdoajarticles https://doi.org/10.4103/1995-7645.281529 2022-12-30T22:08:30Z Objective: To investigate the antimicrobial resistance patterns and prevalence of integrons in Shigella species isolated from children with diarrhea in southwest Iran. Methods: In this study, 1 530 stool samples were collected from children under 15 years with diarrhea referred to teaching hospitals in Ahvaz and Abadan, southwest Iran. Shigella spp. were identified by standard biochemical tests and PCR. The antibiotic resistance pattern of all Shigella isolates was determined by the disk diffusion method and minimum inhibitory concentration (MIC) by E-test. Results: Of 1 530 stool samples, 91 (5.9%, 91/1 530) were positive for Shigella spp. the most common Shigella isolates were Shigella flexneri 47 (51.6%, 47/1 530). Antibiotic susceptibility tests showed that the highest antibiotic resistance was related to trimethoprim-sulfamethoxazole (87.9%, 80/91) and ampicillin (86.8%, 79/91). Multiplex PCR results revealed that 56% and 86.9% of Shigella isolates carried integron class I and integron class II genes, respectively. None of the isolates included the integron class III gene. Conclusions: The high prevalence of multi-drug resistance in Shigella isolates in our area increases the concerns about dissemination of the antibiotic-resistant isolates in this bacterium. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Medicine 14 2 78 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
integrons shigella spp. multi-drug resistance pcr Arctic medicine. Tropical medicine RC955-962 |
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integrons shigella spp. multi-drug resistance pcr Arctic medicine. Tropical medicine RC955-962 Nabi Jomehzadeh Maryam Afzali Khadijeh Ahmadi Shokrollah Salmanzadeh Fateme Jahangiri Mehr Antimicrobial resistance patterns and prevalence of integrons in Shigella species isolated from children with diarrhea in southwest Iran |
topic_facet |
integrons shigella spp. multi-drug resistance pcr Arctic medicine. Tropical medicine RC955-962 |
description |
Objective: To investigate the antimicrobial resistance patterns and prevalence of integrons in Shigella species isolated from children with diarrhea in southwest Iran. Methods: In this study, 1 530 stool samples were collected from children under 15 years with diarrhea referred to teaching hospitals in Ahvaz and Abadan, southwest Iran. Shigella spp. were identified by standard biochemical tests and PCR. The antibiotic resistance pattern of all Shigella isolates was determined by the disk diffusion method and minimum inhibitory concentration (MIC) by E-test. Results: Of 1 530 stool samples, 91 (5.9%, 91/1 530) were positive for Shigella spp. the most common Shigella isolates were Shigella flexneri 47 (51.6%, 47/1 530). Antibiotic susceptibility tests showed that the highest antibiotic resistance was related to trimethoprim-sulfamethoxazole (87.9%, 80/91) and ampicillin (86.8%, 79/91). Multiplex PCR results revealed that 56% and 86.9% of Shigella isolates carried integron class I and integron class II genes, respectively. None of the isolates included the integron class III gene. Conclusions: The high prevalence of multi-drug resistance in Shigella isolates in our area increases the concerns about dissemination of the antibiotic-resistant isolates in this bacterium. |
format |
Article in Journal/Newspaper |
author |
Nabi Jomehzadeh Maryam Afzali Khadijeh Ahmadi Shokrollah Salmanzadeh Fateme Jahangiri Mehr |
author_facet |
Nabi Jomehzadeh Maryam Afzali Khadijeh Ahmadi Shokrollah Salmanzadeh Fateme Jahangiri Mehr |
author_sort |
Nabi Jomehzadeh |
title |
Antimicrobial resistance patterns and prevalence of integrons in Shigella species isolated from children with diarrhea in southwest Iran |
title_short |
Antimicrobial resistance patterns and prevalence of integrons in Shigella species isolated from children with diarrhea in southwest Iran |
title_full |
Antimicrobial resistance patterns and prevalence of integrons in Shigella species isolated from children with diarrhea in southwest Iran |
title_fullStr |
Antimicrobial resistance patterns and prevalence of integrons in Shigella species isolated from children with diarrhea in southwest Iran |
title_full_unstemmed |
Antimicrobial resistance patterns and prevalence of integrons in Shigella species isolated from children with diarrhea in southwest Iran |
title_sort |
antimicrobial resistance patterns and prevalence of integrons in shigella species isolated from children with diarrhea in southwest iran |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2021 |
url |
https://doi.org/10.4103/1995-7645.281529 https://doaj.org/article/7ade4721edd8441a8374018d496ec095 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Medicine, Vol 14, Iss 2, Pp 78-82 (2021) |
op_relation |
http://www.apjtm.org/article.asp?issn=1995-7645;year=2021;volume=14;issue=2;spage=78;epage=82;aulast=Jomehzadeh https://doaj.org/toc/2352-4146 2352-4146 doi:10.4103/1995-7645.281529 https://doaj.org/article/7ade4721edd8441a8374018d496ec095 |
op_doi |
https://doi.org/10.4103/1995-7645.281529 |
container_title |
Asian Pacific Journal of Tropical Medicine |
container_volume |
14 |
container_issue |
2 |
container_start_page |
78 |
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1766339258396704768 |