Toxins from the Caribbean sea anemone Bunodeopsis globulifera increase cisplatin-induced cytotoxicity of lung adenocarcinoma cells
Background Lung cancer causes 1.4 million deaths worldwide while non-small-cell lung cancer (NSCLC) represents 80-85% of the cases. Cisplatin is a standard chemotherapy against this type of cancer; however, tumor cell resistance to this drug limits its efficacy. Sea anemones produce compounds with p...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
---|---|
Main Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
SciELO
2013
|
Subjects: | |
Online Access: | https://doi.org/10.1186/1678-9199-19-12 https://doaj.org/article/7ad104b738844330a4a350a51b895df4 |
id |
ftdoajarticles:oai:doaj.org/article:7ad104b738844330a4a350a51b895df4 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:7ad104b738844330a4a350a51b895df4 2023-05-15T15:11:17+02:00 Toxins from the Caribbean sea anemone Bunodeopsis globulifera increase cisplatin-induced cytotoxicity of lung adenocarcinoma cells Heidi I Monroy-Estrada Yolanda I Chirino Irma E Soria-Mercado Judith Sánchez-Rodríguez 2013-05-01T00:00:00Z https://doi.org/10.1186/1678-9199-19-12 https://doaj.org/article/7ad104b738844330a4a350a51b895df4 EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100308&lng=en&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1186/1678-9199-19-12 https://doaj.org/article/7ad104b738844330a4a350a51b895df4 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 19, Iss 0 (2013) Cnidaria Pharmacology Human lung cancer cells Cytotoxicity assay Cisplatin efficacy Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2013 ftdoajarticles https://doi.org/10.1186/1678-9199-19-12 2022-12-31T04:12:48Z Background Lung cancer causes 1.4 million deaths worldwide while non-small-cell lung cancer (NSCLC) represents 80-85% of the cases. Cisplatin is a standard chemotherapy against this type of cancer; however, tumor cell resistance to this drug limits its efficacy. Sea anemones produce compounds with pharmacological activities that may be useful for augmenting cisplatin efficacy. This study aimed to evaluate the pharmacological activities of crude venom (CV) from the sea anemone Bunodeopsis globulifera and four derived fractions (F1, F2, F3 and F4) to test their increase efficiency cisplatin cytotoxicity in human lung adenocarcinoma cells. Results Pre-exposure to CV, F1 and F2 fractions increases cisplatin cytotoxicity in human lung adenocarcinoma cells under specific conditions. Exposure to CV at 50 μgmL-1 induced a reduction of approximately 50% in cell viability, while a similar cytotoxic effect was observed when cell culture was exposed to F1 at 25 μgmL -1 or F2 at 50 μgmL-1. The cell culture exposure to F1 (10 μgmL-1) fraction combined with cisplatine (25 μM) provoked a decrease in MTT reduction until 65.57% while F2 (25 μgmL-1) fraction combined with cisplatin (10 μM) provoked a decrease in MTT reduction of 72.55%. Conclusions The F1 fraction had the greatest effect on the lung adenocarcinoma cell line compared with CV and F2. The combination of antineoplastic drugs and sea anemone toxins might allow a reduction of chemotherapeutic doses and thus mitigate side effects. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 19 1 12 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Cnidaria Pharmacology Human lung cancer cells Cytotoxicity assay Cisplatin efficacy Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
Cnidaria Pharmacology Human lung cancer cells Cytotoxicity assay Cisplatin efficacy Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 Heidi I Monroy-Estrada Yolanda I Chirino Irma E Soria-Mercado Judith Sánchez-Rodríguez Toxins from the Caribbean sea anemone Bunodeopsis globulifera increase cisplatin-induced cytotoxicity of lung adenocarcinoma cells |
topic_facet |
Cnidaria Pharmacology Human lung cancer cells Cytotoxicity assay Cisplatin efficacy Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Background Lung cancer causes 1.4 million deaths worldwide while non-small-cell lung cancer (NSCLC) represents 80-85% of the cases. Cisplatin is a standard chemotherapy against this type of cancer; however, tumor cell resistance to this drug limits its efficacy. Sea anemones produce compounds with pharmacological activities that may be useful for augmenting cisplatin efficacy. This study aimed to evaluate the pharmacological activities of crude venom (CV) from the sea anemone Bunodeopsis globulifera and four derived fractions (F1, F2, F3 and F4) to test their increase efficiency cisplatin cytotoxicity in human lung adenocarcinoma cells. Results Pre-exposure to CV, F1 and F2 fractions increases cisplatin cytotoxicity in human lung adenocarcinoma cells under specific conditions. Exposure to CV at 50 μgmL-1 induced a reduction of approximately 50% in cell viability, while a similar cytotoxic effect was observed when cell culture was exposed to F1 at 25 μgmL -1 or F2 at 50 μgmL-1. The cell culture exposure to F1 (10 μgmL-1) fraction combined with cisplatine (25 μM) provoked a decrease in MTT reduction until 65.57% while F2 (25 μgmL-1) fraction combined with cisplatin (10 μM) provoked a decrease in MTT reduction of 72.55%. Conclusions The F1 fraction had the greatest effect on the lung adenocarcinoma cell line compared with CV and F2. The combination of antineoplastic drugs and sea anemone toxins might allow a reduction of chemotherapeutic doses and thus mitigate side effects. |
format |
Article in Journal/Newspaper |
author |
Heidi I Monroy-Estrada Yolanda I Chirino Irma E Soria-Mercado Judith Sánchez-Rodríguez |
author_facet |
Heidi I Monroy-Estrada Yolanda I Chirino Irma E Soria-Mercado Judith Sánchez-Rodríguez |
author_sort |
Heidi I Monroy-Estrada |
title |
Toxins from the Caribbean sea anemone Bunodeopsis globulifera increase cisplatin-induced cytotoxicity of lung adenocarcinoma cells |
title_short |
Toxins from the Caribbean sea anemone Bunodeopsis globulifera increase cisplatin-induced cytotoxicity of lung adenocarcinoma cells |
title_full |
Toxins from the Caribbean sea anemone Bunodeopsis globulifera increase cisplatin-induced cytotoxicity of lung adenocarcinoma cells |
title_fullStr |
Toxins from the Caribbean sea anemone Bunodeopsis globulifera increase cisplatin-induced cytotoxicity of lung adenocarcinoma cells |
title_full_unstemmed |
Toxins from the Caribbean sea anemone Bunodeopsis globulifera increase cisplatin-induced cytotoxicity of lung adenocarcinoma cells |
title_sort |
toxins from the caribbean sea anemone bunodeopsis globulifera increase cisplatin-induced cytotoxicity of lung adenocarcinoma cells |
publisher |
SciELO |
publishDate |
2013 |
url |
https://doi.org/10.1186/1678-9199-19-12 https://doaj.org/article/7ad104b738844330a4a350a51b895df4 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 19, Iss 0 (2013) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100308&lng=en&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1186/1678-9199-19-12 https://doaj.org/article/7ad104b738844330a4a350a51b895df4 |
op_doi |
https://doi.org/10.1186/1678-9199-19-12 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
19 |
container_issue |
1 |
container_start_page |
12 |
_version_ |
1766342169921060864 |