Presence of two alternative kdr -like mutations, L1014F and L1014S, and a novel mutation, V1010L, in the voltage gated Na + channel of Anopheles culicifacies from Orissa, India
Abstract Background Knockdown resistance in insects resulting from mutation(s) in the voltage gated Na + channel (VGSC) is one of the mechanisms of resistance against DDT and pyrethroids. Recently a point mutation leading to Leu-to-Phe substitution in the VGSC at residue 1014, a most common kdr muta...
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ftdoajarticles:oai:doaj.org/article:7ab34fbb96f94c709f2328914d4ad999 2023-05-15T15:17:39+02:00 Presence of two alternative kdr -like mutations, L1014F and L1014S, and a novel mutation, V1010L, in the voltage gated Na + channel of Anopheles culicifacies from Orissa, India Bhatt Rajendra M Pradhan Sabyasachi Das Manoj K Dykes Cherry L Singh Om P Agrawal Om P Adak Tridibes 2010-05-01T00:00:00Z https://doi.org/10.1186/1475-2875-9-146 https://doaj.org/article/7ab34fbb96f94c709f2328914d4ad999 EN eng BMC http://www.malariajournal.com/content/9/1/146 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-9-146 1475-2875 https://doaj.org/article/7ab34fbb96f94c709f2328914d4ad999 Malaria Journal, Vol 9, Iss 1, p 146 (2010) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2010 ftdoajarticles https://doi.org/10.1186/1475-2875-9-146 2022-12-31T08:50:03Z Abstract Background Knockdown resistance in insects resulting from mutation(s) in the voltage gated Na + channel (VGSC) is one of the mechanisms of resistance against DDT and pyrethroids. Recently a point mutation leading to Leu-to-Phe substitution in the VGSC at residue 1014, a most common kdr mutation in insects, was reported in Anopheles culicifacies -a major malaria vector in the Indian subcontinent. This study reports the presence of two additional amino acid substitutions in the VGSC of an An. culicifacies population from Malkangiri district of Orissa, India. Methods Anopheles culicifacies sensu lato (s.l.) samples, collected from a population of Malkangiri district of Orissa (India), were sequenced for part of the second transmembrane segment of VGSC and analyzed for the presence of non-synonymous mutations. A new primer introduced restriction analysis-PCR (PIRA-PCR) was developed for the detection of the new mutation L1014S. The An. culicifacies population was genotyped for the presence of L1014F substitution by an amplification refractory mutation system (ARMS) and for L1014S substitutions by using a new PIRA-PCR developed in this study. The results were validated through DNA sequencing. Results DNA sequencing of An. culicifacies individuals collected from district Malkangiri revealed the presence of three amino acid substitutions in the IIS6 transmembrane segments of VGSC, each one resulting from a single point mutation. Two alternative point mutations, 3042A>T transversion or 3041T>C transition, were found at residue L1014 leading to Leu (TTA)-to-Phe (TTT) or -Ser (TCA) changes, respectively. A third and novel substitution, Val (GTG)-to-Leu (TTG or CTG), was identified at residue V1010 resulting from either of the two transversions–3028G>T or 3028G>C. The L1014S substitution co-existed with V1010L in all the samples analyzed irrespective of the type of point mutation associated with the latter. The PIRA-PCR strategy developed for the identification of the new mutation L1014S was found ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Indian Malaria Journal 9 1 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Bhatt Rajendra M Pradhan Sabyasachi Das Manoj K Dykes Cherry L Singh Om P Agrawal Om P Adak Tridibes Presence of two alternative kdr -like mutations, L1014F and L1014S, and a novel mutation, V1010L, in the voltage gated Na + channel of Anopheles culicifacies from Orissa, India |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Knockdown resistance in insects resulting from mutation(s) in the voltage gated Na + channel (VGSC) is one of the mechanisms of resistance against DDT and pyrethroids. Recently a point mutation leading to Leu-to-Phe substitution in the VGSC at residue 1014, a most common kdr mutation in insects, was reported in Anopheles culicifacies -a major malaria vector in the Indian subcontinent. This study reports the presence of two additional amino acid substitutions in the VGSC of an An. culicifacies population from Malkangiri district of Orissa, India. Methods Anopheles culicifacies sensu lato (s.l.) samples, collected from a population of Malkangiri district of Orissa (India), were sequenced for part of the second transmembrane segment of VGSC and analyzed for the presence of non-synonymous mutations. A new primer introduced restriction analysis-PCR (PIRA-PCR) was developed for the detection of the new mutation L1014S. The An. culicifacies population was genotyped for the presence of L1014F substitution by an amplification refractory mutation system (ARMS) and for L1014S substitutions by using a new PIRA-PCR developed in this study. The results were validated through DNA sequencing. Results DNA sequencing of An. culicifacies individuals collected from district Malkangiri revealed the presence of three amino acid substitutions in the IIS6 transmembrane segments of VGSC, each one resulting from a single point mutation. Two alternative point mutations, 3042A>T transversion or 3041T>C transition, were found at residue L1014 leading to Leu (TTA)-to-Phe (TTT) or -Ser (TCA) changes, respectively. A third and novel substitution, Val (GTG)-to-Leu (TTG or CTG), was identified at residue V1010 resulting from either of the two transversions–3028G>T or 3028G>C. The L1014S substitution co-existed with V1010L in all the samples analyzed irrespective of the type of point mutation associated with the latter. The PIRA-PCR strategy developed for the identification of the new mutation L1014S was found ... |
format |
Article in Journal/Newspaper |
author |
Bhatt Rajendra M Pradhan Sabyasachi Das Manoj K Dykes Cherry L Singh Om P Agrawal Om P Adak Tridibes |
author_facet |
Bhatt Rajendra M Pradhan Sabyasachi Das Manoj K Dykes Cherry L Singh Om P Agrawal Om P Adak Tridibes |
author_sort |
Bhatt Rajendra M |
title |
Presence of two alternative kdr -like mutations, L1014F and L1014S, and a novel mutation, V1010L, in the voltage gated Na + channel of Anopheles culicifacies from Orissa, India |
title_short |
Presence of two alternative kdr -like mutations, L1014F and L1014S, and a novel mutation, V1010L, in the voltage gated Na + channel of Anopheles culicifacies from Orissa, India |
title_full |
Presence of two alternative kdr -like mutations, L1014F and L1014S, and a novel mutation, V1010L, in the voltage gated Na + channel of Anopheles culicifacies from Orissa, India |
title_fullStr |
Presence of two alternative kdr -like mutations, L1014F and L1014S, and a novel mutation, V1010L, in the voltage gated Na + channel of Anopheles culicifacies from Orissa, India |
title_full_unstemmed |
Presence of two alternative kdr -like mutations, L1014F and L1014S, and a novel mutation, V1010L, in the voltage gated Na + channel of Anopheles culicifacies from Orissa, India |
title_sort |
presence of two alternative kdr -like mutations, l1014f and l1014s, and a novel mutation, v1010l, in the voltage gated na + channel of anopheles culicifacies from orissa, india |
publisher |
BMC |
publishDate |
2010 |
url |
https://doi.org/10.1186/1475-2875-9-146 https://doaj.org/article/7ab34fbb96f94c709f2328914d4ad999 |
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Arctic Indian |
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Arctic Indian |
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Arctic |
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Arctic |
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Malaria Journal, Vol 9, Iss 1, p 146 (2010) |
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http://www.malariajournal.com/content/9/1/146 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-9-146 1475-2875 https://doaj.org/article/7ab34fbb96f94c709f2328914d4ad999 |
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https://doi.org/10.1186/1475-2875-9-146 |
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Malaria Journal |
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