Molecular Cloning and Polymorphism Analysis of PmFGF18 from Pinctada fucata martensii
Fibroblast growth factor 18 (FGF18) plays an important functional role in skeletal growth and development. The FGF18 gene was characterized in pearl oyster Pinctada fucata martensii ( PmFGF18 ) with the full-length sequence containing an open reading frame of 714 bp encoding 237 amino acids. The dom...
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ftdoajarticles:oai:doaj.org/article:7aaa25cdd03241d28747bd90adba0937 2023-05-15T15:58:40+02:00 Molecular Cloning and Polymorphism Analysis of PmFGF18 from Pinctada fucata martensii Ruijuan Hao Chuchu Mo Linda Adzigbli Chuangye Yang Yuewen Deng Qingheng Wang 2020-11-01T00:00:00Z https://doi.org/10.3390/jmse8110896 https://doaj.org/article/7aaa25cdd03241d28747bd90adba0937 EN eng MDPI AG https://www.mdpi.com/2077-1312/8/11/896 https://doaj.org/toc/2077-1312 doi:10.3390/jmse8110896 2077-1312 https://doaj.org/article/7aaa25cdd03241d28747bd90adba0937 Journal of Marine Science and Engineering, Vol 8, Iss 896, p 896 (2020) FGF18 SNP growth traits Pinctada fucata martensii Naval architecture. Shipbuilding. Marine engineering VM1-989 Oceanography GC1-1581 article 2020 ftdoajarticles https://doi.org/10.3390/jmse8110896 2022-12-31T12:04:53Z Fibroblast growth factor 18 (FGF18) plays an important functional role in skeletal growth and development. The FGF18 gene was characterized in pearl oyster Pinctada fucata martensii ( PmFGF18 ) with the full-length sequence containing an open reading frame of 714 bp encoding 237 amino acids. The domain analysis of PmFGF18 showed a distinctive FGF domain, with a high similarity to FGF18 protein sequences from Crassostrea gigas (43.35%) and C. virginica (37.43%). PmFGF18 expression was revealed in all analyzed tissues with a significantly higher expression level in the fast-growing group than the slow-growing group. The analysis of PmFGF18 polymorphism demonstrated 33 SNPs (single nucleotide polymorphisms) in the CDS and promoter region of PmFGF18 sequence. Association analysis revealed 19 SNPs (2 SNPs from CDS and 17 SNPs from the promoter region) associating significantly with growth traits. Among the associated SNPs, one SNP g.50918198 A > C was verified in the other breeding line. Therefore, PmFGF18 can be utilized as a candidate gene for growth, and its related SNPs could be used in selective breeding of P. f. martensii for the improvement of growth traits. Article in Journal/Newspaper Crassostrea gigas Directory of Open Access Journals: DOAJ Articles Journal of Marine Science and Engineering 8 11 896 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
FGF18 SNP growth traits Pinctada fucata martensii Naval architecture. Shipbuilding. Marine engineering VM1-989 Oceanography GC1-1581 |
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FGF18 SNP growth traits Pinctada fucata martensii Naval architecture. Shipbuilding. Marine engineering VM1-989 Oceanography GC1-1581 Ruijuan Hao Chuchu Mo Linda Adzigbli Chuangye Yang Yuewen Deng Qingheng Wang Molecular Cloning and Polymorphism Analysis of PmFGF18 from Pinctada fucata martensii |
topic_facet |
FGF18 SNP growth traits Pinctada fucata martensii Naval architecture. Shipbuilding. Marine engineering VM1-989 Oceanography GC1-1581 |
description |
Fibroblast growth factor 18 (FGF18) plays an important functional role in skeletal growth and development. The FGF18 gene was characterized in pearl oyster Pinctada fucata martensii ( PmFGF18 ) with the full-length sequence containing an open reading frame of 714 bp encoding 237 amino acids. The domain analysis of PmFGF18 showed a distinctive FGF domain, with a high similarity to FGF18 protein sequences from Crassostrea gigas (43.35%) and C. virginica (37.43%). PmFGF18 expression was revealed in all analyzed tissues with a significantly higher expression level in the fast-growing group than the slow-growing group. The analysis of PmFGF18 polymorphism demonstrated 33 SNPs (single nucleotide polymorphisms) in the CDS and promoter region of PmFGF18 sequence. Association analysis revealed 19 SNPs (2 SNPs from CDS and 17 SNPs from the promoter region) associating significantly with growth traits. Among the associated SNPs, one SNP g.50918198 A > C was verified in the other breeding line. Therefore, PmFGF18 can be utilized as a candidate gene for growth, and its related SNPs could be used in selective breeding of P. f. martensii for the improvement of growth traits. |
format |
Article in Journal/Newspaper |
author |
Ruijuan Hao Chuchu Mo Linda Adzigbli Chuangye Yang Yuewen Deng Qingheng Wang |
author_facet |
Ruijuan Hao Chuchu Mo Linda Adzigbli Chuangye Yang Yuewen Deng Qingheng Wang |
author_sort |
Ruijuan Hao |
title |
Molecular Cloning and Polymorphism Analysis of PmFGF18 from Pinctada fucata martensii |
title_short |
Molecular Cloning and Polymorphism Analysis of PmFGF18 from Pinctada fucata martensii |
title_full |
Molecular Cloning and Polymorphism Analysis of PmFGF18 from Pinctada fucata martensii |
title_fullStr |
Molecular Cloning and Polymorphism Analysis of PmFGF18 from Pinctada fucata martensii |
title_full_unstemmed |
Molecular Cloning and Polymorphism Analysis of PmFGF18 from Pinctada fucata martensii |
title_sort |
molecular cloning and polymorphism analysis of pmfgf18 from pinctada fucata martensii |
publisher |
MDPI AG |
publishDate |
2020 |
url |
https://doi.org/10.3390/jmse8110896 https://doaj.org/article/7aaa25cdd03241d28747bd90adba0937 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Journal of Marine Science and Engineering, Vol 8, Iss 896, p 896 (2020) |
op_relation |
https://www.mdpi.com/2077-1312/8/11/896 https://doaj.org/toc/2077-1312 doi:10.3390/jmse8110896 2077-1312 https://doaj.org/article/7aaa25cdd03241d28747bd90adba0937 |
op_doi |
https://doi.org/10.3390/jmse8110896 |
container_title |
Journal of Marine Science and Engineering |
container_volume |
8 |
container_issue |
11 |
container_start_page |
896 |
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1766394440989016064 |