Chemotherapeutic control of Gram-positive infection in white sea bream (Diplodus sargus, Linnaeus 1758) broodstock
Aim: This study aimed to identify the pathogenic bacteria responsible for the septicemic disease affecting white sea bream brooders and determining the sensitivity of the recovered isolates to different antibiotics followed by estimation of long-acting oxytetracycline (OTC) efficacy in controlling t...
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ftdoajarticles:oai:doaj.org/article:7aa75fc2bb53491a9aa449e271d6b2ae 2023-05-15T18:43:47+02:00 Chemotherapeutic control of Gram-positive infection in white sea bream (Diplodus sargus, Linnaeus 1758) broodstock Nadia G. M. Ali Ibrahim M. Aboyadak Heba S. El-Sayed 2019-02-01T00:00:00Z https://doi.org/10.14202/vetworld.2019.316-324 https://doaj.org/article/7aa75fc2bb53491a9aa449e271d6b2ae EN eng Veterinary World http://www.veterinaryworld.org/Vol.12/February-2019/20.pdf https://doaj.org/toc/0972-8988 https://doaj.org/toc/2231-0916 doi:10.14202/vetworld.2019.316-324 0972-8988 2231-0916 https://doaj.org/article/7aa75fc2bb53491a9aa449e271d6b2ae Veterinary World, Vol 12, Iss 2, Pp 316-324 (2019) Bacillus cereus histopathology high-performance liquid chromatography oxytetracycline sensitivity Staphylococcus epidermidis Animal culture SF1-1100 Veterinary medicine SF600-1100 article 2019 ftdoajarticles https://doi.org/10.14202/vetworld.2019.316-324 2022-12-31T13:01:14Z Aim: This study aimed to identify the pathogenic bacteria responsible for the septicemic disease affecting white sea bream brooders and determining the sensitivity of the recovered isolates to different antibiotics followed by estimation of long-acting oxytetracycline (OTC) efficacy in controlling this disease, and finally, determining the proper dose regimen. Materials and Methods: Biolog microbial identification system was used for determination of the pathogens which are responsible for this disease. Agar disk diffusion test and minimum inhibitory concentration (MIC) were used to determine the antibiotic susceptibility of recovered isolates. Oxytetracycline (OTC) was used at a dose level of 100 mg/kg body weight for the treatment of diseased fish, and the OTC concentration in the serum samples was determined by high-performance liquid chromatography. Results: Fifteen Staphylococcus epidermidis and 11 Bacillus cereus isolates were recovered from the lesion of muscle, tail, eye, and heart blood. S. epidermidis isolates were sensitive to OTC, ciprofloxacin, enrofloxacin, spiramycin, erythromycin (E), and florfenicol. B. cereus isolates were sensitive to all mentioned antibiotics except E. Based on the MIC test, all B. cereus isolates were sensitive to OTC with MIC ranging between <0.125 and 4 μg/ml and 11 S. epidermidis isolates were sensitive with MIC ranging between <0.125 and 8 μg/ml, while four isolates were resistant. Different degrees of degenerative changes were present in the hepatopancreas, posterior kidney, eye, and skin tissues of diseased fish. Conclusion: Single intraperitoneal injection of long-acting OTC at a dose of 100 mg/kg body weight was effective in termination of S. epidermidis and B. cereus infection in white sea bream (D. sargus) broodstock. Article in Journal/Newspaper White Sea Directory of Open Access Journals: DOAJ Articles White Sea Veterinary World 12 2 316 324 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Bacillus cereus histopathology high-performance liquid chromatography oxytetracycline sensitivity Staphylococcus epidermidis Animal culture SF1-1100 Veterinary medicine SF600-1100 |
spellingShingle |
Bacillus cereus histopathology high-performance liquid chromatography oxytetracycline sensitivity Staphylococcus epidermidis Animal culture SF1-1100 Veterinary medicine SF600-1100 Nadia G. M. Ali Ibrahim M. Aboyadak Heba S. El-Sayed Chemotherapeutic control of Gram-positive infection in white sea bream (Diplodus sargus, Linnaeus 1758) broodstock |
topic_facet |
Bacillus cereus histopathology high-performance liquid chromatography oxytetracycline sensitivity Staphylococcus epidermidis Animal culture SF1-1100 Veterinary medicine SF600-1100 |
description |
Aim: This study aimed to identify the pathogenic bacteria responsible for the septicemic disease affecting white sea bream brooders and determining the sensitivity of the recovered isolates to different antibiotics followed by estimation of long-acting oxytetracycline (OTC) efficacy in controlling this disease, and finally, determining the proper dose regimen. Materials and Methods: Biolog microbial identification system was used for determination of the pathogens which are responsible for this disease. Agar disk diffusion test and minimum inhibitory concentration (MIC) were used to determine the antibiotic susceptibility of recovered isolates. Oxytetracycline (OTC) was used at a dose level of 100 mg/kg body weight for the treatment of diseased fish, and the OTC concentration in the serum samples was determined by high-performance liquid chromatography. Results: Fifteen Staphylococcus epidermidis and 11 Bacillus cereus isolates were recovered from the lesion of muscle, tail, eye, and heart blood. S. epidermidis isolates were sensitive to OTC, ciprofloxacin, enrofloxacin, spiramycin, erythromycin (E), and florfenicol. B. cereus isolates were sensitive to all mentioned antibiotics except E. Based on the MIC test, all B. cereus isolates were sensitive to OTC with MIC ranging between <0.125 and 4 μg/ml and 11 S. epidermidis isolates were sensitive with MIC ranging between <0.125 and 8 μg/ml, while four isolates were resistant. Different degrees of degenerative changes were present in the hepatopancreas, posterior kidney, eye, and skin tissues of diseased fish. Conclusion: Single intraperitoneal injection of long-acting OTC at a dose of 100 mg/kg body weight was effective in termination of S. epidermidis and B. cereus infection in white sea bream (D. sargus) broodstock. |
format |
Article in Journal/Newspaper |
author |
Nadia G. M. Ali Ibrahim M. Aboyadak Heba S. El-Sayed |
author_facet |
Nadia G. M. Ali Ibrahim M. Aboyadak Heba S. El-Sayed |
author_sort |
Nadia G. M. Ali |
title |
Chemotherapeutic control of Gram-positive infection in white sea bream (Diplodus sargus, Linnaeus 1758) broodstock |
title_short |
Chemotherapeutic control of Gram-positive infection in white sea bream (Diplodus sargus, Linnaeus 1758) broodstock |
title_full |
Chemotherapeutic control of Gram-positive infection in white sea bream (Diplodus sargus, Linnaeus 1758) broodstock |
title_fullStr |
Chemotherapeutic control of Gram-positive infection in white sea bream (Diplodus sargus, Linnaeus 1758) broodstock |
title_full_unstemmed |
Chemotherapeutic control of Gram-positive infection in white sea bream (Diplodus sargus, Linnaeus 1758) broodstock |
title_sort |
chemotherapeutic control of gram-positive infection in white sea bream (diplodus sargus, linnaeus 1758) broodstock |
publisher |
Veterinary World |
publishDate |
2019 |
url |
https://doi.org/10.14202/vetworld.2019.316-324 https://doaj.org/article/7aa75fc2bb53491a9aa449e271d6b2ae |
geographic |
White Sea |
geographic_facet |
White Sea |
genre |
White Sea |
genre_facet |
White Sea |
op_source |
Veterinary World, Vol 12, Iss 2, Pp 316-324 (2019) |
op_relation |
http://www.veterinaryworld.org/Vol.12/February-2019/20.pdf https://doaj.org/toc/0972-8988 https://doaj.org/toc/2231-0916 doi:10.14202/vetworld.2019.316-324 0972-8988 2231-0916 https://doaj.org/article/7aa75fc2bb53491a9aa449e271d6b2ae |
op_doi |
https://doi.org/10.14202/vetworld.2019.316-324 |
container_title |
Veterinary World |
container_volume |
12 |
container_issue |
2 |
container_start_page |
316 |
op_container_end_page |
324 |
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1766234280354119680 |