Abundant Atribacteria in deep marine sediment from the Adélie Basin, Antarctica
Bacteria belonging to the newly classified candidate phylum Atribacteria (formerly referred to as OP9 and JS1) are common in anoxic methane-rich sediments. However, the metabolic functions and biogeochemical role of these microorganisms in the subsurface remains unrealized due to the lack of pure cu...
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ftdoajarticles:oai:doaj.org/article:7a696d222fad4a8da02b03cc08c5cb83 2023-05-15T13:57:13+02:00 Abundant Atribacteria in deep marine sediment from the Adélie Basin, Antarctica Stephanie Ann Carr Beth N Orcutt Kevin W Mandernack John R. Spear 2015-08-01T00:00:00Z https://doi.org/10.3389/fmicb.2015.00872 https://doaj.org/article/7a696d222fad4a8da02b03cc08c5cb83 EN eng Frontiers Media S.A. http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00872/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2015.00872 https://doaj.org/article/7a696d222fad4a8da02b03cc08c5cb83 Frontiers in Microbiology, Vol 6 (2015) Methane sediment Geomicrobiology Single Cell Genomics Antarctica Atribacteria Microbiology QR1-502 article 2015 ftdoajarticles https://doi.org/10.3389/fmicb.2015.00872 2022-12-31T13:10:54Z Bacteria belonging to the newly classified candidate phylum Atribacteria (formerly referred to as OP9 and JS1) are common in anoxic methane-rich sediments. However, the metabolic functions and biogeochemical role of these microorganisms in the subsurface remains unrealized due to the lack of pure culture representatives. In this study of deep sediment from Antarctica's Adélie Basin, collected during Expedition 318 of the Integrated Ocean Drilling Program (IODP), Atribacteria-related sequences of the 16S rRNA gene were abundant (up to 51% of the sequences) and steadily increased in relative abundance with depth throughout the methane-rich zones. To better understand the metabolic potential of Atribacteria within this environment, and to compare with phylogenetically distinct Atribacteria from non-deep-sea environments, individual cells were sorted for single cell genomics from sediment collected from 97.41 meters below the seafloor from IODP Hole U1357C. As observed for non-marine Atribacteria, a partial single cell genome suggests a heterotrophic metabolism, with Atribacteria potentially producing fermentation products such as acetate, ethanol and CO2. These products may in turn support methanogens within the sediment microbial community and explain the frequent occurrence of Atribacteria in anoxic methane-rich sediments. This first report of a single cell genome from deep sediment broadens the known diversity within the Atribacteria phylum and highlights the potential role of Atribacteria in carbon cycling in deep sediment. Article in Journal/Newspaper Antarc* Antarctica Directory of Open Access Journals: DOAJ Articles Frontiers in Microbiology 6 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Methane sediment Geomicrobiology Single Cell Genomics Antarctica Atribacteria Microbiology QR1-502 |
spellingShingle |
Methane sediment Geomicrobiology Single Cell Genomics Antarctica Atribacteria Microbiology QR1-502 Stephanie Ann Carr Beth N Orcutt Kevin W Mandernack John R. Spear Abundant Atribacteria in deep marine sediment from the Adélie Basin, Antarctica |
topic_facet |
Methane sediment Geomicrobiology Single Cell Genomics Antarctica Atribacteria Microbiology QR1-502 |
description |
Bacteria belonging to the newly classified candidate phylum Atribacteria (formerly referred to as OP9 and JS1) are common in anoxic methane-rich sediments. However, the metabolic functions and biogeochemical role of these microorganisms in the subsurface remains unrealized due to the lack of pure culture representatives. In this study of deep sediment from Antarctica's Adélie Basin, collected during Expedition 318 of the Integrated Ocean Drilling Program (IODP), Atribacteria-related sequences of the 16S rRNA gene were abundant (up to 51% of the sequences) and steadily increased in relative abundance with depth throughout the methane-rich zones. To better understand the metabolic potential of Atribacteria within this environment, and to compare with phylogenetically distinct Atribacteria from non-deep-sea environments, individual cells were sorted for single cell genomics from sediment collected from 97.41 meters below the seafloor from IODP Hole U1357C. As observed for non-marine Atribacteria, a partial single cell genome suggests a heterotrophic metabolism, with Atribacteria potentially producing fermentation products such as acetate, ethanol and CO2. These products may in turn support methanogens within the sediment microbial community and explain the frequent occurrence of Atribacteria in anoxic methane-rich sediments. This first report of a single cell genome from deep sediment broadens the known diversity within the Atribacteria phylum and highlights the potential role of Atribacteria in carbon cycling in deep sediment. |
format |
Article in Journal/Newspaper |
author |
Stephanie Ann Carr Beth N Orcutt Kevin W Mandernack John R. Spear |
author_facet |
Stephanie Ann Carr Beth N Orcutt Kevin W Mandernack John R. Spear |
author_sort |
Stephanie Ann Carr |
title |
Abundant Atribacteria in deep marine sediment from the Adélie Basin, Antarctica |
title_short |
Abundant Atribacteria in deep marine sediment from the Adélie Basin, Antarctica |
title_full |
Abundant Atribacteria in deep marine sediment from the Adélie Basin, Antarctica |
title_fullStr |
Abundant Atribacteria in deep marine sediment from the Adélie Basin, Antarctica |
title_full_unstemmed |
Abundant Atribacteria in deep marine sediment from the Adélie Basin, Antarctica |
title_sort |
abundant atribacteria in deep marine sediment from the adélie basin, antarctica |
publisher |
Frontiers Media S.A. |
publishDate |
2015 |
url |
https://doi.org/10.3389/fmicb.2015.00872 https://doaj.org/article/7a696d222fad4a8da02b03cc08c5cb83 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Frontiers in Microbiology, Vol 6 (2015) |
op_relation |
http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00872/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2015.00872 https://doaj.org/article/7a696d222fad4a8da02b03cc08c5cb83 |
op_doi |
https://doi.org/10.3389/fmicb.2015.00872 |
container_title |
Frontiers in Microbiology |
container_volume |
6 |
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1766264814113390592 |