A crucial role in fertility for the oyster angiotensin-converting enzyme orthologue CgACE.
Angiotensin-converting enzyme (ACE) is a highly conserved metallopeptidase. In mammals, the somatic isoform governs blood pressure whereas the germinal isoform (tACE) is required for fertility. In Ecdysozoans, ACE-like enzymes are implicated in reproduction. Despite ACE orthologues being present fro...
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ftdoajarticles:oai:doaj.org/article:7a2fedf4be3843f6948da0333e3892a1 2023-05-15T15:58:19+02:00 A crucial role in fertility for the oyster angiotensin-converting enzyme orthologue CgACE. Guillaume Riviere Alexandre Fellous Alban Franco Benoit Bernay Pascal Favrel 2011-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0027833 https://doaj.org/article/7a2fedf4be3843f6948da0333e3892a1 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3235092?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0027833 https://doaj.org/article/7a2fedf4be3843f6948da0333e3892a1 PLoS ONE, Vol 6, Iss 12, p e27833 (2011) Medicine R Science Q article 2011 ftdoajarticles https://doi.org/10.1371/journal.pone.0027833 2022-12-31T01:50:03Z Angiotensin-converting enzyme (ACE) is a highly conserved metallopeptidase. In mammals, the somatic isoform governs blood pressure whereas the germinal isoform (tACE) is required for fertility. In Ecdysozoans, ACE-like enzymes are implicated in reproduction. Despite ACE orthologues being present from bacteria to humans, their function(s) remain(s) unknown in distant organisms such as Lophotrochozoans. In silico analysis of an oyster (Crassostrea gigas) EST library suggested the presence of an ACE orthologue in molluscs. Primer walking and 5'-RACE revealed that the 1.9 kb cDNA encodes CgACE, a 632 amino acid protein displaying a conserved single active site and a putative C-terminal transmembrane anchor, thus resembling human tACE, as supported by molecular modelling. FRET activity assays and Maldi-TOF spectrometry indicated that CgACE is a functional dipeptidyl-carboxypeptidase which is active on Angiotensin I and sensitive to ACE inhibitors and chloride ion concentration. Immunocytochemistry revealed that, as its human counterpart, recombinant CgACE is synthesised as a transmembrane enzyme. RT-qPCR, in-situ hybridization and immunohistochemistry shed light on a tissue, and development stage, specific expression pattern for CgACE, which is increased in the gonad during spermatogenesis. The use of ACE inhibitors in vivo indicates that the dipeptidase activity of CgACE is crucial for the oyster fertilization. Our study demonstrates that a transmembrane active ACE is present in the oyster Crassostrea gigas, and for the first time ascribes a functional role for ACE in Lophotrochozoans. Its biological function in reproduction is conserved from molluscs to humans, a finding of particular evolutionary interest especially since oysters represent the most important aquaculture resource worldwide. Article in Journal/Newspaper Crassostrea gigas Directory of Open Access Journals: DOAJ Articles PLoS ONE 6 12 e27833 |
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Medicine R Science Q Guillaume Riviere Alexandre Fellous Alban Franco Benoit Bernay Pascal Favrel A crucial role in fertility for the oyster angiotensin-converting enzyme orthologue CgACE. |
topic_facet |
Medicine R Science Q |
description |
Angiotensin-converting enzyme (ACE) is a highly conserved metallopeptidase. In mammals, the somatic isoform governs blood pressure whereas the germinal isoform (tACE) is required for fertility. In Ecdysozoans, ACE-like enzymes are implicated in reproduction. Despite ACE orthologues being present from bacteria to humans, their function(s) remain(s) unknown in distant organisms such as Lophotrochozoans. In silico analysis of an oyster (Crassostrea gigas) EST library suggested the presence of an ACE orthologue in molluscs. Primer walking and 5'-RACE revealed that the 1.9 kb cDNA encodes CgACE, a 632 amino acid protein displaying a conserved single active site and a putative C-terminal transmembrane anchor, thus resembling human tACE, as supported by molecular modelling. FRET activity assays and Maldi-TOF spectrometry indicated that CgACE is a functional dipeptidyl-carboxypeptidase which is active on Angiotensin I and sensitive to ACE inhibitors and chloride ion concentration. Immunocytochemistry revealed that, as its human counterpart, recombinant CgACE is synthesised as a transmembrane enzyme. RT-qPCR, in-situ hybridization and immunohistochemistry shed light on a tissue, and development stage, specific expression pattern for CgACE, which is increased in the gonad during spermatogenesis. The use of ACE inhibitors in vivo indicates that the dipeptidase activity of CgACE is crucial for the oyster fertilization. Our study demonstrates that a transmembrane active ACE is present in the oyster Crassostrea gigas, and for the first time ascribes a functional role for ACE in Lophotrochozoans. Its biological function in reproduction is conserved from molluscs to humans, a finding of particular evolutionary interest especially since oysters represent the most important aquaculture resource worldwide. |
format |
Article in Journal/Newspaper |
author |
Guillaume Riviere Alexandre Fellous Alban Franco Benoit Bernay Pascal Favrel |
author_facet |
Guillaume Riviere Alexandre Fellous Alban Franco Benoit Bernay Pascal Favrel |
author_sort |
Guillaume Riviere |
title |
A crucial role in fertility for the oyster angiotensin-converting enzyme orthologue CgACE. |
title_short |
A crucial role in fertility for the oyster angiotensin-converting enzyme orthologue CgACE. |
title_full |
A crucial role in fertility for the oyster angiotensin-converting enzyme orthologue CgACE. |
title_fullStr |
A crucial role in fertility for the oyster angiotensin-converting enzyme orthologue CgACE. |
title_full_unstemmed |
A crucial role in fertility for the oyster angiotensin-converting enzyme orthologue CgACE. |
title_sort |
crucial role in fertility for the oyster angiotensin-converting enzyme orthologue cgace. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2011 |
url |
https://doi.org/10.1371/journal.pone.0027833 https://doaj.org/article/7a2fedf4be3843f6948da0333e3892a1 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
PLoS ONE, Vol 6, Iss 12, p e27833 (2011) |
op_relation |
http://europepmc.org/articles/PMC3235092?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0027833 https://doaj.org/article/7a2fedf4be3843f6948da0333e3892a1 |
op_doi |
https://doi.org/10.1371/journal.pone.0027833 |
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PLoS ONE |
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6 |
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12 |
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e27833 |
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