Assessment of Astaxanthin Accumulation in Hepatocytes of Atlantic Salmon Fed Different Diets Using NMR Spectroscopy
This study aimed to assess the astaxanthin (Ax) accumulation in hepatocytes isolated from farmed Atlantic salmon fed different diets (rich marine, poor, poor with marine phospholipids (MPL) and poor with docosahexaenoic acid (DHA)). Nuclear magnetic resonance (NMR) spectroscopy was used for the Ax d...
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MDPI AG
2020
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Online Access: | https://doi.org/10.3390/molecules25081769 https://doaj.org/article/79f27b8ea9be4a1eb82b835a1edb188b |
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ftdoajarticles:oai:doaj.org/article:79f27b8ea9be4a1eb82b835a1edb188b 2023-05-15T15:29:53+02:00 Assessment of Astaxanthin Accumulation in Hepatocytes of Atlantic Salmon Fed Different Diets Using NMR Spectroscopy Elena Shumilina Alessandra Ciampa Trine Ytrestøyl Alexander Dikiy 2020-04-01T00:00:00Z https://doi.org/10.3390/molecules25081769 https://doaj.org/article/79f27b8ea9be4a1eb82b835a1edb188b EN eng MDPI AG https://www.mdpi.com/1420-3049/25/8/1769 https://doaj.org/toc/1420-3049 doi:10.3390/molecules25081769 1420-3049 https://doaj.org/article/79f27b8ea9be4a1eb82b835a1edb188b Molecules, Vol 25, Iss 1769, p 1769 (2020) carotenoid astaxanthin Atlantic salmon fish diet fish feed plant proteins Organic chemistry QD241-441 article 2020 ftdoajarticles https://doi.org/10.3390/molecules25081769 2022-12-31T10:34:33Z This study aimed to assess the astaxanthin (Ax) accumulation in hepatocytes isolated from farmed Atlantic salmon fed different diets (rich marine, poor, poor with marine phospholipids (MPL) and poor with docosahexaenoic acid (DHA)). Nuclear magnetic resonance (NMR) spectroscopy was used for the Ax detection and quantification. The use of the 13 C-enriched Ax allowed the assessment of short-time Ax metabolism. The substitution of fish oil and meal in fish feed on plant analogs and the addition of MPL caused further catabolism and decrease of Ax accumulation in hepatocytes from 17 to about 6 mg/kg or to almost zero in the case of DHA addition. Signals assignment of the native and 13 C-enriched astaxanthin in acetone were performed using 1D and 2D NMR spectra. Article in Journal/Newspaper Atlantic salmon Directory of Open Access Journals: DOAJ Articles Molecules 25 8 1769 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
carotenoid astaxanthin Atlantic salmon fish diet fish feed plant proteins Organic chemistry QD241-441 |
spellingShingle |
carotenoid astaxanthin Atlantic salmon fish diet fish feed plant proteins Organic chemistry QD241-441 Elena Shumilina Alessandra Ciampa Trine Ytrestøyl Alexander Dikiy Assessment of Astaxanthin Accumulation in Hepatocytes of Atlantic Salmon Fed Different Diets Using NMR Spectroscopy |
topic_facet |
carotenoid astaxanthin Atlantic salmon fish diet fish feed plant proteins Organic chemistry QD241-441 |
description |
This study aimed to assess the astaxanthin (Ax) accumulation in hepatocytes isolated from farmed Atlantic salmon fed different diets (rich marine, poor, poor with marine phospholipids (MPL) and poor with docosahexaenoic acid (DHA)). Nuclear magnetic resonance (NMR) spectroscopy was used for the Ax detection and quantification. The use of the 13 C-enriched Ax allowed the assessment of short-time Ax metabolism. The substitution of fish oil and meal in fish feed on plant analogs and the addition of MPL caused further catabolism and decrease of Ax accumulation in hepatocytes from 17 to about 6 mg/kg or to almost zero in the case of DHA addition. Signals assignment of the native and 13 C-enriched astaxanthin in acetone were performed using 1D and 2D NMR spectra. |
format |
Article in Journal/Newspaper |
author |
Elena Shumilina Alessandra Ciampa Trine Ytrestøyl Alexander Dikiy |
author_facet |
Elena Shumilina Alessandra Ciampa Trine Ytrestøyl Alexander Dikiy |
author_sort |
Elena Shumilina |
title |
Assessment of Astaxanthin Accumulation in Hepatocytes of Atlantic Salmon Fed Different Diets Using NMR Spectroscopy |
title_short |
Assessment of Astaxanthin Accumulation in Hepatocytes of Atlantic Salmon Fed Different Diets Using NMR Spectroscopy |
title_full |
Assessment of Astaxanthin Accumulation in Hepatocytes of Atlantic Salmon Fed Different Diets Using NMR Spectroscopy |
title_fullStr |
Assessment of Astaxanthin Accumulation in Hepatocytes of Atlantic Salmon Fed Different Diets Using NMR Spectroscopy |
title_full_unstemmed |
Assessment of Astaxanthin Accumulation in Hepatocytes of Atlantic Salmon Fed Different Diets Using NMR Spectroscopy |
title_sort |
assessment of astaxanthin accumulation in hepatocytes of atlantic salmon fed different diets using nmr spectroscopy |
publisher |
MDPI AG |
publishDate |
2020 |
url |
https://doi.org/10.3390/molecules25081769 https://doaj.org/article/79f27b8ea9be4a1eb82b835a1edb188b |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Molecules, Vol 25, Iss 1769, p 1769 (2020) |
op_relation |
https://www.mdpi.com/1420-3049/25/8/1769 https://doaj.org/toc/1420-3049 doi:10.3390/molecules25081769 1420-3049 https://doaj.org/article/79f27b8ea9be4a1eb82b835a1edb188b |
op_doi |
https://doi.org/10.3390/molecules25081769 |
container_title |
Molecules |
container_volume |
25 |
container_issue |
8 |
container_start_page |
1769 |
_version_ |
1766360331915886592 |