Evidence of an antimicrobial-immunomodulatory role of Atlantic salmon cathelicidins during infection with Yersinia ruckeri.
Cathelicidins are a family of antimicrobial peptides that act as effector molecules of the innate immune system with broad-spectrum antimicrobial properties. These evolutionary conserved cationic host-defence peptides are integral components of the immune response of fish, which are generally believ...
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ftdoajarticles:oai:doaj.org/article:79e5cc4399534fa1b67e6d1c0d371d00 2023-05-15T15:31:12+02:00 Evidence of an antimicrobial-immunomodulatory role of Atlantic salmon cathelicidins during infection with Yersinia ruckeri. Andrew Bridle Elizabeth Nosworthy Mark Polinski Barbara Nowak 2011-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0023417 https://doaj.org/article/79e5cc4399534fa1b67e6d1c0d371d00 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3153500?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0023417 https://doaj.org/article/79e5cc4399534fa1b67e6d1c0d371d00 PLoS ONE, Vol 6, Iss 8, p e23417 (2011) Medicine R Science Q article 2011 ftdoajarticles https://doi.org/10.1371/journal.pone.0023417 2022-12-31T12:50:34Z Cathelicidins are a family of antimicrobial peptides that act as effector molecules of the innate immune system with broad-spectrum antimicrobial properties. These evolutionary conserved cationic host-defence peptides are integral components of the immune response of fish, which are generally believed to rely heavily on innate immune defences to invading pathogens. In this study we showed that Atlantic salmon cathelicidin 1 and 2 (asCATH1 and asCATH2) stimulated peripheral blood leukocytes increasing the transcription of the chemokine interleukin-8. Further, functional differences were identified between the two cathelicidins. In the presence of serum, asCATH1 displayed greatly diminished host haemolytic activity, while the constitutively expressed asCATH2 had no haemolytic activity with or without serum. These findings support our hypothesis that fish cathelicidins exert their primary antimicrobial action at the site of pathogen invasion such as epithelial surfaces. Further, we hypothesise that like their mammalian counterparts in the presence of serum they act as mediators of the innate and adaptive immune response via the release of cytokines thus indirectly protecting against a variety of pathogens. We highlight the importance of this immunomodulatory role from the involvement of asCATHs during an infection with the fish pathogen Yersinia ruckeri. While we were able to demonstrate in vitro that asCATH1 and 2, possessed direct microbicidal activity against the fish pathogen, Vibrio anguillarum, and a common gram negative bacterium, Escherichia coli, little or no bactericidal activity was found against Y. ruckeri. The contribution of either asCATH in the immune response or as a potential virulence factor during yersiniosis is highlighted from the increased expression of asCATH1 and 2 mRNA during an in vivo challenge with Y. ruckeri . We propose that Atlantic salmon cathelicidins participate in the interplay between the innate and adaptive immune systems via the release of cytokines enabling a more effective ... Article in Journal/Newspaper Atlantic salmon Directory of Open Access Journals: DOAJ Articles PLoS ONE 6 8 e23417 |
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Medicine R Science Q |
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Medicine R Science Q Andrew Bridle Elizabeth Nosworthy Mark Polinski Barbara Nowak Evidence of an antimicrobial-immunomodulatory role of Atlantic salmon cathelicidins during infection with Yersinia ruckeri. |
topic_facet |
Medicine R Science Q |
description |
Cathelicidins are a family of antimicrobial peptides that act as effector molecules of the innate immune system with broad-spectrum antimicrobial properties. These evolutionary conserved cationic host-defence peptides are integral components of the immune response of fish, which are generally believed to rely heavily on innate immune defences to invading pathogens. In this study we showed that Atlantic salmon cathelicidin 1 and 2 (asCATH1 and asCATH2) stimulated peripheral blood leukocytes increasing the transcription of the chemokine interleukin-8. Further, functional differences were identified between the two cathelicidins. In the presence of serum, asCATH1 displayed greatly diminished host haemolytic activity, while the constitutively expressed asCATH2 had no haemolytic activity with or without serum. These findings support our hypothesis that fish cathelicidins exert their primary antimicrobial action at the site of pathogen invasion such as epithelial surfaces. Further, we hypothesise that like their mammalian counterparts in the presence of serum they act as mediators of the innate and adaptive immune response via the release of cytokines thus indirectly protecting against a variety of pathogens. We highlight the importance of this immunomodulatory role from the involvement of asCATHs during an infection with the fish pathogen Yersinia ruckeri. While we were able to demonstrate in vitro that asCATH1 and 2, possessed direct microbicidal activity against the fish pathogen, Vibrio anguillarum, and a common gram negative bacterium, Escherichia coli, little or no bactericidal activity was found against Y. ruckeri. The contribution of either asCATH in the immune response or as a potential virulence factor during yersiniosis is highlighted from the increased expression of asCATH1 and 2 mRNA during an in vivo challenge with Y. ruckeri . We propose that Atlantic salmon cathelicidins participate in the interplay between the innate and adaptive immune systems via the release of cytokines enabling a more effective ... |
format |
Article in Journal/Newspaper |
author |
Andrew Bridle Elizabeth Nosworthy Mark Polinski Barbara Nowak |
author_facet |
Andrew Bridle Elizabeth Nosworthy Mark Polinski Barbara Nowak |
author_sort |
Andrew Bridle |
title |
Evidence of an antimicrobial-immunomodulatory role of Atlantic salmon cathelicidins during infection with Yersinia ruckeri. |
title_short |
Evidence of an antimicrobial-immunomodulatory role of Atlantic salmon cathelicidins during infection with Yersinia ruckeri. |
title_full |
Evidence of an antimicrobial-immunomodulatory role of Atlantic salmon cathelicidins during infection with Yersinia ruckeri. |
title_fullStr |
Evidence of an antimicrobial-immunomodulatory role of Atlantic salmon cathelicidins during infection with Yersinia ruckeri. |
title_full_unstemmed |
Evidence of an antimicrobial-immunomodulatory role of Atlantic salmon cathelicidins during infection with Yersinia ruckeri. |
title_sort |
evidence of an antimicrobial-immunomodulatory role of atlantic salmon cathelicidins during infection with yersinia ruckeri. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2011 |
url |
https://doi.org/10.1371/journal.pone.0023417 https://doaj.org/article/79e5cc4399534fa1b67e6d1c0d371d00 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
PLoS ONE, Vol 6, Iss 8, p e23417 (2011) |
op_relation |
http://europepmc.org/articles/PMC3153500?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0023417 https://doaj.org/article/79e5cc4399534fa1b67e6d1c0d371d00 |
op_doi |
https://doi.org/10.1371/journal.pone.0023417 |
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PLoS ONE |
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6 |
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8 |
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e23417 |
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