No evidence of amplified Plasmodium falciparum plasmepsin II gene copy number in an area with artemisinin-resistant malaria along the China–Myanmar border
Abstract Background The emergence and spread of artemisinin resistance in Plasmodium falciparum poses a threat to malaria eradication, including China’s plan to eliminate malaria by 2020. Piperaquine (PPQ) resistance has emerged in Cambodia, compromising an important partner drug that is widely used...
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ftdoajarticles:oai:doaj.org/article:79bfe86e4ae7400e8836e00276a1895f 2023-05-15T15:18:06+02:00 No evidence of amplified Plasmodium falciparum plasmepsin II gene copy number in an area with artemisinin-resistant malaria along the China–Myanmar border Fang Huang Biraj Shrestha Hui Liu Lin-Hua Tang Shui-Sen Zhou Xiao-Nong Zhou Shannon Takala-Harrison Pascal Ringwald Myaing M. Nyunt Christopher V. Plowe 2020-09-01T00:00:00Z https://doi.org/10.1186/s12936-020-03410-6 https://doaj.org/article/79bfe86e4ae7400e8836e00276a1895f EN eng BMC http://link.springer.com/article/10.1186/s12936-020-03410-6 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-020-03410-6 1475-2875 https://doaj.org/article/79bfe86e4ae7400e8836e00276a1895f Malaria Journal, Vol 19, Iss 1, Pp 1-9 (2020) Plasmodium falciparum Artemisinin resistance Piperaquine Plasmepsin II China–Myanmar border Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2020 ftdoajarticles https://doi.org/10.1186/s12936-020-03410-6 2022-12-31T00:24:23Z Abstract Background The emergence and spread of artemisinin resistance in Plasmodium falciparum poses a threat to malaria eradication, including China’s plan to eliminate malaria by 2020. Piperaquine (PPQ) resistance has emerged in Cambodia, compromising an important partner drug that is widely used in China in the form of dihydroartemisinin (DHA)-PPQ. Several mutations in a P. falciparum gene encoding a kelch protein on chromosome 13 (k13) are associated with artemisinin resistance and have arisen spread in the Great Mekong subregion, including the China–Myanmar border. Multiple copies of the plasmepsin II/III (pm2/3) genes, located on chromosome 14, have been shown to be associated with PPQ resistance. Methods The therapeutic efficacy of DHA-PPQ for the treatment of uncomplicated P. falciparum was evaluated along the China–Myanmar border from 2010 to 2014. The dry blood spots samples collected in the efficacy study prior DHA-PPQ treatment and from the local hospital by passive detection were used to amplify k13 and pm2. Polymorphisms within k13 were genotyped by capillary sequencing and pm2 copy number was quantified by relative-quantitative real-time polymerase chain reaction. Treatment outcome was evaluated with the World Health Organization protocol. A linear regression model was used to estimate the association between the day 3 positive rate and k13 mutation and the relationship of the pm2 copy number variants and k13 mutations. Results DHA-PPQ was effective for uncomplicated P. falciparum infection in Yunnan Province with cure rates > 95%. Twelve non synonymous mutations in the k13 domain were observed among the 268 samples with the prevalence of 44.0% and the predominant mutation was F446I with a prevalence of 32.8%. Only one sample was observed with multi-copies of pm2, including parasites with and without k13 mutations. The therapeutic efficacy of DHA-PPQ was > 95% along the China–Myanmar border, consistent with the lack of amplification of pm2. Conclusion DHA-PPQ for uncomplicated P. falciparum ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 19 1 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Plasmodium falciparum Artemisinin resistance Piperaquine Plasmepsin II China–Myanmar border Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Plasmodium falciparum Artemisinin resistance Piperaquine Plasmepsin II China–Myanmar border Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Fang Huang Biraj Shrestha Hui Liu Lin-Hua Tang Shui-Sen Zhou Xiao-Nong Zhou Shannon Takala-Harrison Pascal Ringwald Myaing M. Nyunt Christopher V. Plowe No evidence of amplified Plasmodium falciparum plasmepsin II gene copy number in an area with artemisinin-resistant malaria along the China–Myanmar border |
topic_facet |
Plasmodium falciparum Artemisinin resistance Piperaquine Plasmepsin II China–Myanmar border Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background The emergence and spread of artemisinin resistance in Plasmodium falciparum poses a threat to malaria eradication, including China’s plan to eliminate malaria by 2020. Piperaquine (PPQ) resistance has emerged in Cambodia, compromising an important partner drug that is widely used in China in the form of dihydroartemisinin (DHA)-PPQ. Several mutations in a P. falciparum gene encoding a kelch protein on chromosome 13 (k13) are associated with artemisinin resistance and have arisen spread in the Great Mekong subregion, including the China–Myanmar border. Multiple copies of the plasmepsin II/III (pm2/3) genes, located on chromosome 14, have been shown to be associated with PPQ resistance. Methods The therapeutic efficacy of DHA-PPQ for the treatment of uncomplicated P. falciparum was evaluated along the China–Myanmar border from 2010 to 2014. The dry blood spots samples collected in the efficacy study prior DHA-PPQ treatment and from the local hospital by passive detection were used to amplify k13 and pm2. Polymorphisms within k13 were genotyped by capillary sequencing and pm2 copy number was quantified by relative-quantitative real-time polymerase chain reaction. Treatment outcome was evaluated with the World Health Organization protocol. A linear regression model was used to estimate the association between the day 3 positive rate and k13 mutation and the relationship of the pm2 copy number variants and k13 mutations. Results DHA-PPQ was effective for uncomplicated P. falciparum infection in Yunnan Province with cure rates > 95%. Twelve non synonymous mutations in the k13 domain were observed among the 268 samples with the prevalence of 44.0% and the predominant mutation was F446I with a prevalence of 32.8%. Only one sample was observed with multi-copies of pm2, including parasites with and without k13 mutations. The therapeutic efficacy of DHA-PPQ was > 95% along the China–Myanmar border, consistent with the lack of amplification of pm2. Conclusion DHA-PPQ for uncomplicated P. falciparum ... |
format |
Article in Journal/Newspaper |
author |
Fang Huang Biraj Shrestha Hui Liu Lin-Hua Tang Shui-Sen Zhou Xiao-Nong Zhou Shannon Takala-Harrison Pascal Ringwald Myaing M. Nyunt Christopher V. Plowe |
author_facet |
Fang Huang Biraj Shrestha Hui Liu Lin-Hua Tang Shui-Sen Zhou Xiao-Nong Zhou Shannon Takala-Harrison Pascal Ringwald Myaing M. Nyunt Christopher V. Plowe |
author_sort |
Fang Huang |
title |
No evidence of amplified Plasmodium falciparum plasmepsin II gene copy number in an area with artemisinin-resistant malaria along the China–Myanmar border |
title_short |
No evidence of amplified Plasmodium falciparum plasmepsin II gene copy number in an area with artemisinin-resistant malaria along the China–Myanmar border |
title_full |
No evidence of amplified Plasmodium falciparum plasmepsin II gene copy number in an area with artemisinin-resistant malaria along the China–Myanmar border |
title_fullStr |
No evidence of amplified Plasmodium falciparum plasmepsin II gene copy number in an area with artemisinin-resistant malaria along the China–Myanmar border |
title_full_unstemmed |
No evidence of amplified Plasmodium falciparum plasmepsin II gene copy number in an area with artemisinin-resistant malaria along the China–Myanmar border |
title_sort |
no evidence of amplified plasmodium falciparum plasmepsin ii gene copy number in an area with artemisinin-resistant malaria along the china–myanmar border |
publisher |
BMC |
publishDate |
2020 |
url |
https://doi.org/10.1186/s12936-020-03410-6 https://doaj.org/article/79bfe86e4ae7400e8836e00276a1895f |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 19, Iss 1, Pp 1-9 (2020) |
op_relation |
http://link.springer.com/article/10.1186/s12936-020-03410-6 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-020-03410-6 1475-2875 https://doaj.org/article/79bfe86e4ae7400e8836e00276a1895f |
op_doi |
https://doi.org/10.1186/s12936-020-03410-6 |
container_title |
Malaria Journal |
container_volume |
19 |
container_issue |
1 |
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1766348338749243392 |