Evaluation of PCR Approaches for Detection of Bartonella bacilliformis in Blood Samples.
BACKGROUND:The lack of an effective diagnostic tool for Carrion's disease leads to misdiagnosis, wrong treatments and perpetuation of asymptomatic carriers living in endemic areas. Conventional PCR approaches have been reported as a diagnostic technique. However, the detection limit of these te...
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ftdoajarticles:oai:doaj.org/article:79a92dcda860462d9ba9be5f0a21d879 2023-05-15T15:08:59+02:00 Evaluation of PCR Approaches for Detection of Bartonella bacilliformis in Blood Samples. Cláudia Gomes Sandra Martinez-Puchol Maria J Pons Jorge Bazán Carmen Tinco Juana del Valle Joaquim Ruiz 2016-03-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0004529 https://doaj.org/article/79a92dcda860462d9ba9be5f0a21d879 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4784731?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004529 https://doaj.org/article/79a92dcda860462d9ba9be5f0a21d879 PLoS Neglected Tropical Diseases, Vol 10, Iss 3, p e0004529 (2016) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2016 ftdoajarticles https://doi.org/10.1371/journal.pntd.0004529 2022-12-31T15:54:44Z BACKGROUND:The lack of an effective diagnostic tool for Carrion's disease leads to misdiagnosis, wrong treatments and perpetuation of asymptomatic carriers living in endemic areas. Conventional PCR approaches have been reported as a diagnostic technique. However, the detection limit of these techniques is not clear as well as if its usefulness in low bacteriemia cases. The aim of this study was to evaluate the detection limit of 3 PCR approaches. METHODOLOGY/PRINCIPAL FINDINGS:We determined the detection limit of 3 different PCR approaches: Bartonella-specific 16S rRNA, fla and its genes. We also evaluated the viability of dry blood spots to be used as a sample transport system. Our results show that 16S rRNA PCR is the approach with a lowest detection limit, 5 CFU/μL, and thus, the best diagnostic PCR tool studied. Dry blood spots diminish the sensitivity of the assay. CONCLUSIONS/SIGNIFICANCE:From the tested PCRs, the 16S rRNA PCR-approach is the best to be used in the direct blood detection of acute cases of Carrion's disease. However its use in samples from dry blood spots results in easier management of transport samples in rural areas, a slight decrease in the sensitivity was observed. The usefulness to detect by PCR the presence of low-bacteriemic or asymptomatic carriers is doubtful, showing the need to search for new more sensible techniques. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 10 3 e0004529 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Cláudia Gomes Sandra Martinez-Puchol Maria J Pons Jorge Bazán Carmen Tinco Juana del Valle Joaquim Ruiz Evaluation of PCR Approaches for Detection of Bartonella bacilliformis in Blood Samples. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND:The lack of an effective diagnostic tool for Carrion's disease leads to misdiagnosis, wrong treatments and perpetuation of asymptomatic carriers living in endemic areas. Conventional PCR approaches have been reported as a diagnostic technique. However, the detection limit of these techniques is not clear as well as if its usefulness in low bacteriemia cases. The aim of this study was to evaluate the detection limit of 3 PCR approaches. METHODOLOGY/PRINCIPAL FINDINGS:We determined the detection limit of 3 different PCR approaches: Bartonella-specific 16S rRNA, fla and its genes. We also evaluated the viability of dry blood spots to be used as a sample transport system. Our results show that 16S rRNA PCR is the approach with a lowest detection limit, 5 CFU/μL, and thus, the best diagnostic PCR tool studied. Dry blood spots diminish the sensitivity of the assay. CONCLUSIONS/SIGNIFICANCE:From the tested PCRs, the 16S rRNA PCR-approach is the best to be used in the direct blood detection of acute cases of Carrion's disease. However its use in samples from dry blood spots results in easier management of transport samples in rural areas, a slight decrease in the sensitivity was observed. The usefulness to detect by PCR the presence of low-bacteriemic or asymptomatic carriers is doubtful, showing the need to search for new more sensible techniques. |
format |
Article in Journal/Newspaper |
author |
Cláudia Gomes Sandra Martinez-Puchol Maria J Pons Jorge Bazán Carmen Tinco Juana del Valle Joaquim Ruiz |
author_facet |
Cláudia Gomes Sandra Martinez-Puchol Maria J Pons Jorge Bazán Carmen Tinco Juana del Valle Joaquim Ruiz |
author_sort |
Cláudia Gomes |
title |
Evaluation of PCR Approaches for Detection of Bartonella bacilliformis in Blood Samples. |
title_short |
Evaluation of PCR Approaches for Detection of Bartonella bacilliformis in Blood Samples. |
title_full |
Evaluation of PCR Approaches for Detection of Bartonella bacilliformis in Blood Samples. |
title_fullStr |
Evaluation of PCR Approaches for Detection of Bartonella bacilliformis in Blood Samples. |
title_full_unstemmed |
Evaluation of PCR Approaches for Detection of Bartonella bacilliformis in Blood Samples. |
title_sort |
evaluation of pcr approaches for detection of bartonella bacilliformis in blood samples. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2016 |
url |
https://doi.org/10.1371/journal.pntd.0004529 https://doaj.org/article/79a92dcda860462d9ba9be5f0a21d879 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 10, Iss 3, p e0004529 (2016) |
op_relation |
http://europepmc.org/articles/PMC4784731?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004529 https://doaj.org/article/79a92dcda860462d9ba9be5f0a21d879 |
op_doi |
https://doi.org/10.1371/journal.pntd.0004529 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
10 |
container_issue |
3 |
container_start_page |
e0004529 |
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1766340245725380608 |