Circulation of West Nile virus in mosquitoes approximate to the migratory bird stopover in West Coast Malaysia.
Being a tropical country with a conducive environment for mosquitoes, mosquito-borne illnesses such as dengue, chikungunya, lymphatic filariasis, malaria, and Japanese encephalitis are prevalent in Malaysia. Recent studies reported asymptomatic infection of West Nile virus (WNV) in animals and human...
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ftdoajarticles:oai:doaj.org/article:79a1126af350447182cb704413636490 2023-06-11T04:09:36+02:00 Circulation of West Nile virus in mosquitoes approximate to the migratory bird stopover in West Coast Malaysia. Jafar Ali Natasha Abd Rahaman Yasmin Reuben Sunil Kumar Sharma Saulol Hamid Nur-Fazila Md Isa Nur-Mahiza Siti Suri Arshad Hussni Omar Mohammed Kiven Kumar Shih Keng Loong Mohd Kharip Shah Ahmad Khusaini 2023-04-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0011255 https://doaj.org/article/79a1126af350447182cb704413636490 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0011255 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0011255 https://doaj.org/article/79a1126af350447182cb704413636490 PLoS Neglected Tropical Diseases, Vol 17, Iss 4, p e0011255 (2023) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2023 ftdoajarticles https://doi.org/10.1371/journal.pntd.0011255 2023-05-07T00:31:57Z Being a tropical country with a conducive environment for mosquitoes, mosquito-borne illnesses such as dengue, chikungunya, lymphatic filariasis, malaria, and Japanese encephalitis are prevalent in Malaysia. Recent studies reported asymptomatic infection of West Nile virus (WNV) in animals and humans, but none of the studies included mosquitoes, except for one report made half a century ago. Considering the scarcity of information, our study sampled mosquitoes near migratory bird stopover wetland areas of West Coast Malaysia located in the Kuala Gula Bird Sanctuary and Kapar Energy Venture, during the southward migration period in October 2017 and September 2018. Our previous publication reported that migratory birds were positive for WNV antibody and RNA. Using a nested RT-PCR analysis, WNV RNA was detected in 35 (12.8%) out of 285 mosquito pools consisting of 2,635 mosquitoes, most of which were Culex spp. (species). Sanger sequencing and phylogenetic analysis revealed that the sequences grouped within lineage 2 and shared 90.12%-97.01% similarity with sequences found locally as well as those from Africa, Germany, Romania, Italy, and Israel. Evidence of WNV in the mosquitoes substantiates the need for continued surveillance of WNV in Malaysia. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 17 4 e0011255 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Jafar Ali Natasha Abd Rahaman Yasmin Reuben Sunil Kumar Sharma Saulol Hamid Nur-Fazila Md Isa Nur-Mahiza Siti Suri Arshad Hussni Omar Mohammed Kiven Kumar Shih Keng Loong Mohd Kharip Shah Ahmad Khusaini Circulation of West Nile virus in mosquitoes approximate to the migratory bird stopover in West Coast Malaysia. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Being a tropical country with a conducive environment for mosquitoes, mosquito-borne illnesses such as dengue, chikungunya, lymphatic filariasis, malaria, and Japanese encephalitis are prevalent in Malaysia. Recent studies reported asymptomatic infection of West Nile virus (WNV) in animals and humans, but none of the studies included mosquitoes, except for one report made half a century ago. Considering the scarcity of information, our study sampled mosquitoes near migratory bird stopover wetland areas of West Coast Malaysia located in the Kuala Gula Bird Sanctuary and Kapar Energy Venture, during the southward migration period in October 2017 and September 2018. Our previous publication reported that migratory birds were positive for WNV antibody and RNA. Using a nested RT-PCR analysis, WNV RNA was detected in 35 (12.8%) out of 285 mosquito pools consisting of 2,635 mosquitoes, most of which were Culex spp. (species). Sanger sequencing and phylogenetic analysis revealed that the sequences grouped within lineage 2 and shared 90.12%-97.01% similarity with sequences found locally as well as those from Africa, Germany, Romania, Italy, and Israel. Evidence of WNV in the mosquitoes substantiates the need for continued surveillance of WNV in Malaysia. |
format |
Article in Journal/Newspaper |
author |
Jafar Ali Natasha Abd Rahaman Yasmin Reuben Sunil Kumar Sharma Saulol Hamid Nur-Fazila Md Isa Nur-Mahiza Siti Suri Arshad Hussni Omar Mohammed Kiven Kumar Shih Keng Loong Mohd Kharip Shah Ahmad Khusaini |
author_facet |
Jafar Ali Natasha Abd Rahaman Yasmin Reuben Sunil Kumar Sharma Saulol Hamid Nur-Fazila Md Isa Nur-Mahiza Siti Suri Arshad Hussni Omar Mohammed Kiven Kumar Shih Keng Loong Mohd Kharip Shah Ahmad Khusaini |
author_sort |
Jafar Ali Natasha |
title |
Circulation of West Nile virus in mosquitoes approximate to the migratory bird stopover in West Coast Malaysia. |
title_short |
Circulation of West Nile virus in mosquitoes approximate to the migratory bird stopover in West Coast Malaysia. |
title_full |
Circulation of West Nile virus in mosquitoes approximate to the migratory bird stopover in West Coast Malaysia. |
title_fullStr |
Circulation of West Nile virus in mosquitoes approximate to the migratory bird stopover in West Coast Malaysia. |
title_full_unstemmed |
Circulation of West Nile virus in mosquitoes approximate to the migratory bird stopover in West Coast Malaysia. |
title_sort |
circulation of west nile virus in mosquitoes approximate to the migratory bird stopover in west coast malaysia. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2023 |
url |
https://doi.org/10.1371/journal.pntd.0011255 https://doaj.org/article/79a1126af350447182cb704413636490 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 17, Iss 4, p e0011255 (2023) |
op_relation |
https://doi.org/10.1371/journal.pntd.0011255 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0011255 https://doaj.org/article/79a1126af350447182cb704413636490 |
op_doi |
https://doi.org/10.1371/journal.pntd.0011255 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
17 |
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4 |
container_start_page |
e0011255 |
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1768383557608144896 |