Phenotypic and genotypic profile of pyrethroid resistance in populations of the mosquito Aedes aegypti from Goiânia, Central West Brazil

• Published in: Revista da Sociedade Brasileira de Medicina Tropical
• Main Authors: Francesca Guaracyaba Garcia Chapadense, Everton Kort Kamp Fernandes, José Bento Pereira Lima, Ademir Jesus Martins, Luana Carrara Silva, Welington Tristão da Rocha, Adelair Helena dos Santos, Pedro Cravo
• Format: Article in Journal/Newspaper
• Language: English
• Published: Sociedade Brasileira de Medicina Tropical (SBMT) 2015
• Subjects: Aedes aegypti; Resistance; Voltage-gated sodium channel; Arctic medicine. Tropical medicine; RC955-962; Arctic
• Online Access: https://doi.org/10.1590/0037-8682-0046-2015; https://doaj.org/article/7963af0e5ce5460a8ecc96001417cadc

ABSTRACTINTRODUCTION:The mosquito Aedes aegypti has evolved resistance to pyrethroid insecticides. The present study evaluated Ae. aegypti from Goiânia for the resistant phenotype and for mutations associated with resistance.METHODS:Insecticide dose-response bioassays were conducted on mosquitoes descended from field-collected eggs, and polymerase chain reaction (PCR) was used to genotype 90 individuals at sites implicated in pyrethroid resistance.RESULTS:All mosquito populations displayed high levels of resistance to deltamethrin, as well as high frequencies of the 1016Ile kdr and 1534Cys kdrmutations.CONCLUSIONS:Aedes aegypti populations in the Goiânia area are highly resistant to deltamethrin, presumably due to high frequencies of kdr(knockdown-resistance) mutations.