Screening blood-fed mosquitoes for the diagnosis of filarioid helminths and avian malaria
Abstract Background Both Dirofilaria repens and recently D. immitis are known to be endemic in Hungary. As one of several recent cases, the fatal case of a dog infested with D. immitis in Szeged, Southern Hungary, received attention from the media. Hence it was decided to catch mosquitoes in the gar...
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ftdoajarticles:oai:doaj.org/article:795bd966a3444873b2a2553169344e6f 2023-07-02T03:33:53+02:00 Screening blood-fed mosquitoes for the diagnosis of filarioid helminths and avian malaria Carina Zittra Zsanett Kocziha Szilárd Pinnyei Josef Harl Katrin Kieser Alice Laciny Barbara Eigner Katja Silbermayr Georg G Duscher Éva Fok Hans-Peter Fuehrer 2015-01-01T00:00:00Z https://doi.org/10.1186/s13071-015-0637-4 https://doaj.org/article/795bd966a3444873b2a2553169344e6f EN eng BMC https://doi.org/10.1186/s13071-015-0637-4 https://doaj.org/toc/1756-3305 doi:10.1186/s13071-015-0637-4 1756-3305 https://doaj.org/article/795bd966a3444873b2a2553169344e6f Parasites & Vectors, Vol 8, Iss 1, Pp 1-6 (2015) Culicidae Dirofilaria Setaria tundra Blood-fed Avian malaria Cytochrome c oxidase I Infectious and parasitic diseases RC109-216 article 2015 ftdoajarticles https://doi.org/10.1186/s13071-015-0637-4 2023-06-11T00:37:44Z Abstract Background Both Dirofilaria repens and recently D. immitis are known to be endemic in Hungary. As one of several recent cases, the fatal case of a dog infested with D. immitis in Szeged, Southern Hungary, received attention from the media. Hence it was decided to catch mosquitoes in the garden where the dog lived to screen for filarioid helminths and Plasmodium spp. using molecular tools. Methods Mosquitoes were caught in Szeged, in the garden where the infected dog was kept, in July 2013 with M-360 electric mosquito traps and were stored in ethanol until further procedure. Female mosquitoes were classified to genus level by morphology. Each mosquito was homogenized and analyzed for filarioid helminths and avian malaria using standardized PCR techniques. Positive mosquito samples were further identified to species level by comparing a section of the mitochondrial COI gene to GenBank® entries. Results In this study, 267 blood-fed mosquitoes were caught in July 2013 in Szeged. Subsequent molecular screening revealed that not only D. immitis was present in the analyzed specimens but also DNA of D. repens, Setaria tundra and Plasmodium spp. was confirmed. Conclusions The analysis of blood-fed mosquitoes for the diagnosis of Dirofilaria spp. and other mosquito-borne pathogens seems to be an adequate technique to evaluate if filarioid helminths are present in a certain area. Usually only unfed female mosquitoes are analyzed for epidemiological studies. However, blood-fed mosquitoes can only be used for screening if a pathogen is present because the role of the mosquito as vector cannot be classified (blood of bitten host). Furthermore, Setaria tundra was confirmed for the first time in Hungary. Article in Journal/Newspaper Tundra Directory of Open Access Journals: DOAJ Articles Parasites & Vectors 8 1 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Culicidae Dirofilaria Setaria tundra Blood-fed Avian malaria Cytochrome c oxidase I Infectious and parasitic diseases RC109-216 |
spellingShingle |
Culicidae Dirofilaria Setaria tundra Blood-fed Avian malaria Cytochrome c oxidase I Infectious and parasitic diseases RC109-216 Carina Zittra Zsanett Kocziha Szilárd Pinnyei Josef Harl Katrin Kieser Alice Laciny Barbara Eigner Katja Silbermayr Georg G Duscher Éva Fok Hans-Peter Fuehrer Screening blood-fed mosquitoes for the diagnosis of filarioid helminths and avian malaria |
topic_facet |
Culicidae Dirofilaria Setaria tundra Blood-fed Avian malaria Cytochrome c oxidase I Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Both Dirofilaria repens and recently D. immitis are known to be endemic in Hungary. As one of several recent cases, the fatal case of a dog infested with D. immitis in Szeged, Southern Hungary, received attention from the media. Hence it was decided to catch mosquitoes in the garden where the dog lived to screen for filarioid helminths and Plasmodium spp. using molecular tools. Methods Mosquitoes were caught in Szeged, in the garden where the infected dog was kept, in July 2013 with M-360 electric mosquito traps and were stored in ethanol until further procedure. Female mosquitoes were classified to genus level by morphology. Each mosquito was homogenized and analyzed for filarioid helminths and avian malaria using standardized PCR techniques. Positive mosquito samples were further identified to species level by comparing a section of the mitochondrial COI gene to GenBank® entries. Results In this study, 267 blood-fed mosquitoes were caught in July 2013 in Szeged. Subsequent molecular screening revealed that not only D. immitis was present in the analyzed specimens but also DNA of D. repens, Setaria tundra and Plasmodium spp. was confirmed. Conclusions The analysis of blood-fed mosquitoes for the diagnosis of Dirofilaria spp. and other mosquito-borne pathogens seems to be an adequate technique to evaluate if filarioid helminths are present in a certain area. Usually only unfed female mosquitoes are analyzed for epidemiological studies. However, blood-fed mosquitoes can only be used for screening if a pathogen is present because the role of the mosquito as vector cannot be classified (blood of bitten host). Furthermore, Setaria tundra was confirmed for the first time in Hungary. |
format |
Article in Journal/Newspaper |
author |
Carina Zittra Zsanett Kocziha Szilárd Pinnyei Josef Harl Katrin Kieser Alice Laciny Barbara Eigner Katja Silbermayr Georg G Duscher Éva Fok Hans-Peter Fuehrer |
author_facet |
Carina Zittra Zsanett Kocziha Szilárd Pinnyei Josef Harl Katrin Kieser Alice Laciny Barbara Eigner Katja Silbermayr Georg G Duscher Éva Fok Hans-Peter Fuehrer |
author_sort |
Carina Zittra |
title |
Screening blood-fed mosquitoes for the diagnosis of filarioid helminths and avian malaria |
title_short |
Screening blood-fed mosquitoes for the diagnosis of filarioid helminths and avian malaria |
title_full |
Screening blood-fed mosquitoes for the diagnosis of filarioid helminths and avian malaria |
title_fullStr |
Screening blood-fed mosquitoes for the diagnosis of filarioid helminths and avian malaria |
title_full_unstemmed |
Screening blood-fed mosquitoes for the diagnosis of filarioid helminths and avian malaria |
title_sort |
screening blood-fed mosquitoes for the diagnosis of filarioid helminths and avian malaria |
publisher |
BMC |
publishDate |
2015 |
url |
https://doi.org/10.1186/s13071-015-0637-4 https://doaj.org/article/795bd966a3444873b2a2553169344e6f |
genre |
Tundra |
genre_facet |
Tundra |
op_source |
Parasites & Vectors, Vol 8, Iss 1, Pp 1-6 (2015) |
op_relation |
https://doi.org/10.1186/s13071-015-0637-4 https://doaj.org/toc/1756-3305 doi:10.1186/s13071-015-0637-4 1756-3305 https://doaj.org/article/795bd966a3444873b2a2553169344e6f |
op_doi |
https://doi.org/10.1186/s13071-015-0637-4 |
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Parasites & Vectors |
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