Isolation and characterization of actinobacteria from Yalujiang coastal wetland, North China
Objective: To evaluate various types of samples from the different marine environments as sources of actinomycetes from the Yalujiang coastal wetland, North China, and to screen their antimicrobial properties. Further, the identified actinomycetes were characterized based on morphological, biochemic...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
---|---|
Main Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wolters Kluwer Medknow Publications
2015
|
Subjects: | |
Online Access: | https://doi.org/10.1016/j.apjtb.2015.04.007 https://doaj.org/article/79576c5f1c3d4434b233199b0ef959e9 |
id |
ftdoajarticles:oai:doaj.org/article:79576c5f1c3d4434b233199b0ef959e9 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:79576c5f1c3d4434b233199b0ef959e9 2023-05-15T15:05:59+02:00 Isolation and characterization of actinobacteria from Yalujiang coastal wetland, North China Jicheng Yu Liu Zhang Qiu Liu Xiaohui Qi Ying Ji Beom Seok Kim 2015-07-01T00:00:00Z https://doi.org/10.1016/j.apjtb.2015.04.007 https://doaj.org/article/79576c5f1c3d4434b233199b0ef959e9 EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169115000921 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2015.04.007 https://doaj.org/article/79576c5f1c3d4434b233199b0ef959e9 Asian Pacific Journal of Tropical Biomedicine, Vol 5, Iss 7, Pp 555-560 (2015) Actinomycetes Antimicrobial activity 16S rRNA Yalujiang coastal wetland Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2015 ftdoajarticles https://doi.org/10.1016/j.apjtb.2015.04.007 2022-12-31T00:00:32Z Objective: To evaluate various types of samples from the different marine environments as sources of actinomycetes from the Yalujiang coastal wetland, North China, and to screen their antimicrobial properties. Further, the identified actinomycetes were characterized based on morphological, biochemical, and physiological characteristics. Methods: Eight different production media were used to isolate actinomycetes from different stations of marine soil sediments in Yalujiang coastal wetland and the genotypic positions were established by 16S rDNA. Results: A total of 172 actinomycetal isolates were obtained from 13 samples using five media. The most effective culture media in the isolation of actinobacteria were Gause's Synthetic agar and Starch-casein agar. Among 172 isolates, 46 isolates (26.74%) showed antibacterial activity, 70.93% belonged to the genus Streptomyces, others were Micromonospora spp. and Rhodococcus spp. Out of the 46 isolates, two cultures were further supported by morphological characterization analysis. Conclusions: This is the first report about actinomycetes isolated from Yalujiang coastal wetland and it seems that the promising isolates from the unusual/unexplored wetland may prove to be an important step in the development of microbial natural product research. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 5 7 555 560 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Actinomycetes Antimicrobial activity 16S rRNA Yalujiang coastal wetland Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
Actinomycetes Antimicrobial activity 16S rRNA Yalujiang coastal wetland Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Jicheng Yu Liu Zhang Qiu Liu Xiaohui Qi Ying Ji Beom Seok Kim Isolation and characterization of actinobacteria from Yalujiang coastal wetland, North China |
topic_facet |
Actinomycetes Antimicrobial activity 16S rRNA Yalujiang coastal wetland Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To evaluate various types of samples from the different marine environments as sources of actinomycetes from the Yalujiang coastal wetland, North China, and to screen their antimicrobial properties. Further, the identified actinomycetes were characterized based on morphological, biochemical, and physiological characteristics. Methods: Eight different production media were used to isolate actinomycetes from different stations of marine soil sediments in Yalujiang coastal wetland and the genotypic positions were established by 16S rDNA. Results: A total of 172 actinomycetal isolates were obtained from 13 samples using five media. The most effective culture media in the isolation of actinobacteria were Gause's Synthetic agar and Starch-casein agar. Among 172 isolates, 46 isolates (26.74%) showed antibacterial activity, 70.93% belonged to the genus Streptomyces, others were Micromonospora spp. and Rhodococcus spp. Out of the 46 isolates, two cultures were further supported by morphological characterization analysis. Conclusions: This is the first report about actinomycetes isolated from Yalujiang coastal wetland and it seems that the promising isolates from the unusual/unexplored wetland may prove to be an important step in the development of microbial natural product research. |
format |
Article in Journal/Newspaper |
author |
Jicheng Yu Liu Zhang Qiu Liu Xiaohui Qi Ying Ji Beom Seok Kim |
author_facet |
Jicheng Yu Liu Zhang Qiu Liu Xiaohui Qi Ying Ji Beom Seok Kim |
author_sort |
Jicheng Yu |
title |
Isolation and characterization of actinobacteria from Yalujiang coastal wetland, North China |
title_short |
Isolation and characterization of actinobacteria from Yalujiang coastal wetland, North China |
title_full |
Isolation and characterization of actinobacteria from Yalujiang coastal wetland, North China |
title_fullStr |
Isolation and characterization of actinobacteria from Yalujiang coastal wetland, North China |
title_full_unstemmed |
Isolation and characterization of actinobacteria from Yalujiang coastal wetland, North China |
title_sort |
isolation and characterization of actinobacteria from yalujiang coastal wetland, north china |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2015 |
url |
https://doi.org/10.1016/j.apjtb.2015.04.007 https://doaj.org/article/79576c5f1c3d4434b233199b0ef959e9 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 5, Iss 7, Pp 555-560 (2015) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169115000921 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2015.04.007 https://doaj.org/article/79576c5f1c3d4434b233199b0ef959e9 |
op_doi |
https://doi.org/10.1016/j.apjtb.2015.04.007 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
5 |
container_issue |
7 |
container_start_page |
555 |
op_container_end_page |
560 |
_version_ |
1766337658673430528 |