Exposure of the Plasmodium falciparum clonally variant STEVOR proteins on the merozoite surface
Abstract Background Plasmodium falciparum merozoites are free invasive forms that invade host erythrocytes in iterative cycles in the presence of different arms of the immune system. Variant antigens are known to play a role in immune evasion and several gene families coding for variant antigens hav...
Published in: | Malaria Journal |
---|---|
Main Authors: | , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
BMC
2011
|
Subjects: | |
Online Access: | https://doi.org/10.1186/1475-2875-10-58 https://doaj.org/article/790537d821e64878b79c7339e5df344f |
id |
ftdoajarticles:oai:doaj.org/article:790537d821e64878b79c7339e5df344f |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:790537d821e64878b79c7339e5df344f 2023-05-15T15:10:02+02:00 Exposure of the Plasmodium falciparum clonally variant STEVOR proteins on the merozoite surface Meri Seppo Khattab Ayman 2011-03-01T00:00:00Z https://doi.org/10.1186/1475-2875-10-58 https://doaj.org/article/790537d821e64878b79c7339e5df344f EN eng BMC http://www.malariajournal.com/content/10/1/58 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-10-58 1475-2875 https://doaj.org/article/790537d821e64878b79c7339e5df344f Malaria Journal, Vol 10, Iss 1, p 58 (2011) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2011 ftdoajarticles https://doi.org/10.1186/1475-2875-10-58 2022-12-30T21:44:50Z Abstract Background Plasmodium falciparum merozoites are free invasive forms that invade host erythrocytes in iterative cycles in the presence of different arms of the immune system. Variant antigens are known to play a role in immune evasion and several gene families coding for variant antigens have been identified in P. falciparum . However, none of them have been reported to be expressed on the surface of merozoites. Methods Flow cytometry, immunofluorescence microscopy, and immunoblotting assays were performed to assess surface exposure, membrane association and stage specific expression of the STEVOR family of variants proteins, respectively. Results Using a polyclonal antibody (anti-PFL2610w) with a broad specificity towards different STEVOR variants, the STEVOR proteins were identified on the surface of non-permeabilized/non-fixed merozoites in flow cytometry assays. Anti-PFL2610w antibody showed that several STEVORs were expressed in the trophozoite stage of the parasite but only one variant was integrated into the merozoite membrane. Moreover, this antibody failed to identify STEVORs on the surface of the parent schizont infected erythrocytes (IE) although they were readily identified when schizont IE were permeabilized. Conclusions These data suggest for a role for STEVOR in immune evasion by P. falciparum merozoites to allow successful invasion of erythrocytes. Additionally, the expression of STEVORs in the schizont stage may only represent a step in the biogenesis process of the merozoite surface coat. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 10 1 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Meri Seppo Khattab Ayman Exposure of the Plasmodium falciparum clonally variant STEVOR proteins on the merozoite surface |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Plasmodium falciparum merozoites are free invasive forms that invade host erythrocytes in iterative cycles in the presence of different arms of the immune system. Variant antigens are known to play a role in immune evasion and several gene families coding for variant antigens have been identified in P. falciparum . However, none of them have been reported to be expressed on the surface of merozoites. Methods Flow cytometry, immunofluorescence microscopy, and immunoblotting assays were performed to assess surface exposure, membrane association and stage specific expression of the STEVOR family of variants proteins, respectively. Results Using a polyclonal antibody (anti-PFL2610w) with a broad specificity towards different STEVOR variants, the STEVOR proteins were identified on the surface of non-permeabilized/non-fixed merozoites in flow cytometry assays. Anti-PFL2610w antibody showed that several STEVORs were expressed in the trophozoite stage of the parasite but only one variant was integrated into the merozoite membrane. Moreover, this antibody failed to identify STEVORs on the surface of the parent schizont infected erythrocytes (IE) although they were readily identified when schizont IE were permeabilized. Conclusions These data suggest for a role for STEVOR in immune evasion by P. falciparum merozoites to allow successful invasion of erythrocytes. Additionally, the expression of STEVORs in the schizont stage may only represent a step in the biogenesis process of the merozoite surface coat. |
format |
Article in Journal/Newspaper |
author |
Meri Seppo Khattab Ayman |
author_facet |
Meri Seppo Khattab Ayman |
author_sort |
Meri Seppo |
title |
Exposure of the Plasmodium falciparum clonally variant STEVOR proteins on the merozoite surface |
title_short |
Exposure of the Plasmodium falciparum clonally variant STEVOR proteins on the merozoite surface |
title_full |
Exposure of the Plasmodium falciparum clonally variant STEVOR proteins on the merozoite surface |
title_fullStr |
Exposure of the Plasmodium falciparum clonally variant STEVOR proteins on the merozoite surface |
title_full_unstemmed |
Exposure of the Plasmodium falciparum clonally variant STEVOR proteins on the merozoite surface |
title_sort |
exposure of the plasmodium falciparum clonally variant stevor proteins on the merozoite surface |
publisher |
BMC |
publishDate |
2011 |
url |
https://doi.org/10.1186/1475-2875-10-58 https://doaj.org/article/790537d821e64878b79c7339e5df344f |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 10, Iss 1, p 58 (2011) |
op_relation |
http://www.malariajournal.com/content/10/1/58 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-10-58 1475-2875 https://doaj.org/article/790537d821e64878b79c7339e5df344f |
op_doi |
https://doi.org/10.1186/1475-2875-10-58 |
container_title |
Malaria Journal |
container_volume |
10 |
container_issue |
1 |
_version_ |
1766341100222545920 |