Anopheles mosquito surveillance in Madagascar reveals multiple blood feeding behavior and Plasmodium infection.
Background The Madagascar National Strategic Plan for Malaria Control 2018 (NSP) outlines malaria control pre-elimination strategies that include detailed goals for mosquito control. Primary surveillance protocols and mosquito control interventions focus on indoor vectors of malaria, while many pote...
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ftdoajarticles:oai:doaj.org/article:7877c05944fb4a508c7cbbb3b33aab36 2023-05-15T15:13:13+02:00 Anopheles mosquito surveillance in Madagascar reveals multiple blood feeding behavior and Plasmodium infection. Riley E Tedrow Tovonahary Rakotomanga Thiery Nepomichene Rosalind E Howes Jocelyn Ratovonjato Arséne C Ratsimbasoa Gavin J Svenson Peter A Zimmerman 2019-07-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0007176 https://doaj.org/article/7877c05944fb4a508c7cbbb3b33aab36 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0007176 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007176 https://doaj.org/article/7877c05944fb4a508c7cbbb3b33aab36 PLoS Neglected Tropical Diseases, Vol 13, Iss 7, p e0007176 (2019) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2019 ftdoajarticles https://doi.org/10.1371/journal.pntd.0007176 2022-12-31T09:15:21Z Background The Madagascar National Strategic Plan for Malaria Control 2018 (NSP) outlines malaria control pre-elimination strategies that include detailed goals for mosquito control. Primary surveillance protocols and mosquito control interventions focus on indoor vectors of malaria, while many potential vectors feed and rest outdoors. Here we describe the application of tools that advance our understanding of diversity, host choice, and Plasmodium infection in the Anopheline mosquitoes of the Western Highland Fringe of Madagascar. Methodology/principal findings We employed a modified barrier screen trap, the QUadrant Enabled Screen Trap (QUEST), in conjunction with the recently developed multiplex BLOOdmeal Detection Assay for Regional Transmission (BLOODART). We captured a total of 1252 female Anopheles mosquitoes (10 species), all of which were subjected to BLOODART analysis. QUEST collection captured a heterogenous distribution of mosquito density, diversity, host choice, and Plasmodium infection. Concordance between Anopheles morphology and BLOODART species identifications ranged from 93-99%. Mosquito feeding behavior in this collection frequently exhibited multiple blood meal hosts (single host = 53.6%, two hosts = 42.1%, three hosts = 4.3%). The overall percentage of human positive bloodmeals increased between the December 2017 and the April 2018 timepoints (27% to 44%). Plasmodium positivity was frequently observed in the abdomens of vectors considered to be of secondary importance, with an overall prevalence of 6%. Conclusions/significance The QUEST was an efficient tool for sampling exophilic Anopheline mosquitoes. Vectors considered to be of secondary importance were commonly found with Plasmodium DNA in their abdomens, indicating a need to account for these species in routine surveillance efforts. Mosquitoes exhibited multiple blood feeding behavior within a gonotrophic cycle, with predominantly non-human hosts in the bloodmeal. Taken together, this complex feeding behavior could enhance the role of ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 13 7 e0007176 |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Riley E Tedrow Tovonahary Rakotomanga Thiery Nepomichene Rosalind E Howes Jocelyn Ratovonjato Arséne C Ratsimbasoa Gavin J Svenson Peter A Zimmerman Anopheles mosquito surveillance in Madagascar reveals multiple blood feeding behavior and Plasmodium infection. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Background The Madagascar National Strategic Plan for Malaria Control 2018 (NSP) outlines malaria control pre-elimination strategies that include detailed goals for mosquito control. Primary surveillance protocols and mosquito control interventions focus on indoor vectors of malaria, while many potential vectors feed and rest outdoors. Here we describe the application of tools that advance our understanding of diversity, host choice, and Plasmodium infection in the Anopheline mosquitoes of the Western Highland Fringe of Madagascar. Methodology/principal findings We employed a modified barrier screen trap, the QUadrant Enabled Screen Trap (QUEST), in conjunction with the recently developed multiplex BLOOdmeal Detection Assay for Regional Transmission (BLOODART). We captured a total of 1252 female Anopheles mosquitoes (10 species), all of which were subjected to BLOODART analysis. QUEST collection captured a heterogenous distribution of mosquito density, diversity, host choice, and Plasmodium infection. Concordance between Anopheles morphology and BLOODART species identifications ranged from 93-99%. Mosquito feeding behavior in this collection frequently exhibited multiple blood meal hosts (single host = 53.6%, two hosts = 42.1%, three hosts = 4.3%). The overall percentage of human positive bloodmeals increased between the December 2017 and the April 2018 timepoints (27% to 44%). Plasmodium positivity was frequently observed in the abdomens of vectors considered to be of secondary importance, with an overall prevalence of 6%. Conclusions/significance The QUEST was an efficient tool for sampling exophilic Anopheline mosquitoes. Vectors considered to be of secondary importance were commonly found with Plasmodium DNA in their abdomens, indicating a need to account for these species in routine surveillance efforts. Mosquitoes exhibited multiple blood feeding behavior within a gonotrophic cycle, with predominantly non-human hosts in the bloodmeal. Taken together, this complex feeding behavior could enhance the role of ... |
format |
Article in Journal/Newspaper |
author |
Riley E Tedrow Tovonahary Rakotomanga Thiery Nepomichene Rosalind E Howes Jocelyn Ratovonjato Arséne C Ratsimbasoa Gavin J Svenson Peter A Zimmerman |
author_facet |
Riley E Tedrow Tovonahary Rakotomanga Thiery Nepomichene Rosalind E Howes Jocelyn Ratovonjato Arséne C Ratsimbasoa Gavin J Svenson Peter A Zimmerman |
author_sort |
Riley E Tedrow |
title |
Anopheles mosquito surveillance in Madagascar reveals multiple blood feeding behavior and Plasmodium infection. |
title_short |
Anopheles mosquito surveillance in Madagascar reveals multiple blood feeding behavior and Plasmodium infection. |
title_full |
Anopheles mosquito surveillance in Madagascar reveals multiple blood feeding behavior and Plasmodium infection. |
title_fullStr |
Anopheles mosquito surveillance in Madagascar reveals multiple blood feeding behavior and Plasmodium infection. |
title_full_unstemmed |
Anopheles mosquito surveillance in Madagascar reveals multiple blood feeding behavior and Plasmodium infection. |
title_sort |
anopheles mosquito surveillance in madagascar reveals multiple blood feeding behavior and plasmodium infection. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2019 |
url |
https://doi.org/10.1371/journal.pntd.0007176 https://doaj.org/article/7877c05944fb4a508c7cbbb3b33aab36 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 13, Iss 7, p e0007176 (2019) |
op_relation |
https://doi.org/10.1371/journal.pntd.0007176 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007176 https://doaj.org/article/7877c05944fb4a508c7cbbb3b33aab36 |
op_doi |
https://doi.org/10.1371/journal.pntd.0007176 |
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PLOS Neglected Tropical Diseases |
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13 |
container_issue |
7 |
container_start_page |
e0007176 |
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