Eukaryotic microorganisms in cold environments. Examples from Pyrenean glaciers
Little is known about the viability of eukaryotic microorganisms preserved in icy regions. Here we report on the diversity of microbial eukaryotes in ice samples derived from four Pyrenean glaciers. The species composition of eukaryotic communities in these glaciers is unknown mostly because of the...
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ftdoajarticles:oai:doaj.org/article:78569f4d413d4468a2637335e8efb582 2023-05-15T16:38:20+02:00 Eukaryotic microorganisms in cold environments. Examples from Pyrenean glaciers Laura eGarcia-Descalzo Eva eGarcia-Lopez Marina ePostigo Fernando eBaquero Alberto eAlcazar Cristina eCid 2013-03-01T00:00:00Z https://doi.org/10.3389/fmicb.2013.00055 https://doaj.org/article/78569f4d413d4468a2637335e8efb582 EN eng Frontiers Media S.A. http://journal.frontiersin.org/Journal/10.3389/fmicb.2013.00055/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2013.00055 https://doaj.org/article/78569f4d413d4468a2637335e8efb582 Frontiers in Microbiology, Vol 4 (2013) Climate Change Metagenomics Biodiversity glaciers Scanning electron microscopy eukaryotic microorganisms Microbiology QR1-502 article 2013 ftdoajarticles https://doi.org/10.3389/fmicb.2013.00055 2022-12-31T01:35:36Z Little is known about the viability of eukaryotic microorganisms preserved in icy regions. Here we report on the diversity of microbial eukaryotes in ice samples derived from four Pyrenean glaciers. The species composition of eukaryotic communities in these glaciers is unknown mostly because of the presence of a multi-year ice cap, and it is not clear whether they harbor the same populations. The recent deglaciation of these areas is allowing an easy access to glacial layers that correspond to the Little Ice Age although some isolated deposits are attributed to previous glacial cycles. In this study, we use molecular 18S rRNA-based approaches to characterize some of the microbial eukaryotic populations associated with Pyrenean glaciers. Firstly, we performed a chemical and microscopical characterization of ice samples. Secondly, molecular analyses revealed interesting protist genetic diversity in glaciers. In order to understand the microbial composition of the ice samples the eukaryotic communities resident in the glacial samples were examined by amplifying community DNA and constructing clone libraries with 18S rRNA primers. After removal of potential chimeric sequences and derreplication of identical sequences, phylogenetic analysis demonstrated that several different protists could be identified. Protist diversity was more phylum rich in Aneto and Monte Perdido glaciers. The dominant taxonomic groups across all samples (> 1 % of all sequences) were Viridiplantae and Rhizaria. Significant variations in relative abundances of protist phyla between higher and lower glaciers were observed. At the genus level, significant differences were also recorded for the dominant genera Chloromonas, Raphidonema , Heteromita , Koliella and Bodomorpha. In addition, protist community structure showed significant differences between glaciers. The relative abundances of protist groups at different taxonomic levels correlated with the altitude and area of glaciers and with pH. Article in Journal/Newspaper Ice cap Directory of Open Access Journals: DOAJ Articles Frontiers in Microbiology 4 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Climate Change Metagenomics Biodiversity glaciers Scanning electron microscopy eukaryotic microorganisms Microbiology QR1-502 |
spellingShingle |
Climate Change Metagenomics Biodiversity glaciers Scanning electron microscopy eukaryotic microorganisms Microbiology QR1-502 Laura eGarcia-Descalzo Eva eGarcia-Lopez Marina ePostigo Fernando eBaquero Alberto eAlcazar Cristina eCid Eukaryotic microorganisms in cold environments. Examples from Pyrenean glaciers |
topic_facet |
Climate Change Metagenomics Biodiversity glaciers Scanning electron microscopy eukaryotic microorganisms Microbiology QR1-502 |
description |
Little is known about the viability of eukaryotic microorganisms preserved in icy regions. Here we report on the diversity of microbial eukaryotes in ice samples derived from four Pyrenean glaciers. The species composition of eukaryotic communities in these glaciers is unknown mostly because of the presence of a multi-year ice cap, and it is not clear whether they harbor the same populations. The recent deglaciation of these areas is allowing an easy access to glacial layers that correspond to the Little Ice Age although some isolated deposits are attributed to previous glacial cycles. In this study, we use molecular 18S rRNA-based approaches to characterize some of the microbial eukaryotic populations associated with Pyrenean glaciers. Firstly, we performed a chemical and microscopical characterization of ice samples. Secondly, molecular analyses revealed interesting protist genetic diversity in glaciers. In order to understand the microbial composition of the ice samples the eukaryotic communities resident in the glacial samples were examined by amplifying community DNA and constructing clone libraries with 18S rRNA primers. After removal of potential chimeric sequences and derreplication of identical sequences, phylogenetic analysis demonstrated that several different protists could be identified. Protist diversity was more phylum rich in Aneto and Monte Perdido glaciers. The dominant taxonomic groups across all samples (> 1 % of all sequences) were Viridiplantae and Rhizaria. Significant variations in relative abundances of protist phyla between higher and lower glaciers were observed. At the genus level, significant differences were also recorded for the dominant genera Chloromonas, Raphidonema , Heteromita , Koliella and Bodomorpha. In addition, protist community structure showed significant differences between glaciers. The relative abundances of protist groups at different taxonomic levels correlated with the altitude and area of glaciers and with pH. |
format |
Article in Journal/Newspaper |
author |
Laura eGarcia-Descalzo Eva eGarcia-Lopez Marina ePostigo Fernando eBaquero Alberto eAlcazar Cristina eCid |
author_facet |
Laura eGarcia-Descalzo Eva eGarcia-Lopez Marina ePostigo Fernando eBaquero Alberto eAlcazar Cristina eCid |
author_sort |
Laura eGarcia-Descalzo |
title |
Eukaryotic microorganisms in cold environments. Examples from Pyrenean glaciers |
title_short |
Eukaryotic microorganisms in cold environments. Examples from Pyrenean glaciers |
title_full |
Eukaryotic microorganisms in cold environments. Examples from Pyrenean glaciers |
title_fullStr |
Eukaryotic microorganisms in cold environments. Examples from Pyrenean glaciers |
title_full_unstemmed |
Eukaryotic microorganisms in cold environments. Examples from Pyrenean glaciers |
title_sort |
eukaryotic microorganisms in cold environments. examples from pyrenean glaciers |
publisher |
Frontiers Media S.A. |
publishDate |
2013 |
url |
https://doi.org/10.3389/fmicb.2013.00055 https://doaj.org/article/78569f4d413d4468a2637335e8efb582 |
genre |
Ice cap |
genre_facet |
Ice cap |
op_source |
Frontiers in Microbiology, Vol 4 (2013) |
op_relation |
http://journal.frontiersin.org/Journal/10.3389/fmicb.2013.00055/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2013.00055 https://doaj.org/article/78569f4d413d4468a2637335e8efb582 |
op_doi |
https://doi.org/10.3389/fmicb.2013.00055 |
container_title |
Frontiers in Microbiology |
container_volume |
4 |
_version_ |
1766028609828421632 |