Accuracy of molecular biology techniques for the diagnosis of Strongyloides stercoralis infection-A systematic review and meta-analysis.
Strongyloides stercoralis infection is a neglected tropical disease which can lead to severe symptoms and even death in immunosuppressed people. Unfortunately, its diagnosis is hampered by the lack of a gold standard, as the sensitivity of traditional parasitological tests (including microscopic exa...
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ftdoajarticles:oai:doaj.org/article:7837700931cf4a00a01c24fa9839abe3 2023-05-15T15:09:39+02:00 Accuracy of molecular biology techniques for the diagnosis of Strongyloides stercoralis infection-A systematic review and meta-analysis. Dora Buonfrate Ana Requena-Mendez Andrea Angheben Michela Cinquini Mario Cruciani Andrea Fittipaldo Giovanni Giorli Federico Gobbi Chiara Piubelli Zeno Bisoffi 2018-02-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0006229 https://doaj.org/article/7837700931cf4a00a01c24fa9839abe3 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5823464?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006229 https://doaj.org/article/7837700931cf4a00a01c24fa9839abe3 PLoS Neglected Tropical Diseases, Vol 12, Iss 2, p e0006229 (2018) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2018 ftdoajarticles https://doi.org/10.1371/journal.pntd.0006229 2022-12-31T01:54:46Z Strongyloides stercoralis infection is a neglected tropical disease which can lead to severe symptoms and even death in immunosuppressed people. Unfortunately, its diagnosis is hampered by the lack of a gold standard, as the sensitivity of traditional parasitological tests (including microscopic examination of stool samples and coproculture) is low. Hence, alternative diagnostic methods, such as molecular biology techniques (mostly polymerase chain reaction, PCR) have been implemented. However, there are discrepancies in the reported accuracy of PCR.A systematic review with meta-analysis was conducted in order to evaluate the accuracy of PCR for the diagnosis of S. stercoralis infection. The protocol was registered with PROSPERO International Prospective Register of Systematic Reviews (record: CRD42016054298). Fourteen studies, 12 of which evaluating real-time PCR, were included in the analysis. The specificity of the techniques resulted high (ranging from 93 to 95%, according to the reference test(s) used). When all molecular techniques were compared to parasitological methods, the sensitivity of PCR was assessed at 71.8% (95% CI 52.2-85.5), that decreased to 61.8% (95% CI 42.0-78.4) when serology was added among the reference tests. Similarly, sensitivity of real-time PCR resulted 64.4% (95% CI 46.2-77.7) when compared to parasitological methods only, 56.5% (95% CI 39.2-72.4) including serology.PCR might not be suitable for screening purpose, whereas it might have a role as a confirmatory test. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 12 2 e0006229 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Dora Buonfrate Ana Requena-Mendez Andrea Angheben Michela Cinquini Mario Cruciani Andrea Fittipaldo Giovanni Giorli Federico Gobbi Chiara Piubelli Zeno Bisoffi Accuracy of molecular biology techniques for the diagnosis of Strongyloides stercoralis infection-A systematic review and meta-analysis. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Strongyloides stercoralis infection is a neglected tropical disease which can lead to severe symptoms and even death in immunosuppressed people. Unfortunately, its diagnosis is hampered by the lack of a gold standard, as the sensitivity of traditional parasitological tests (including microscopic examination of stool samples and coproculture) is low. Hence, alternative diagnostic methods, such as molecular biology techniques (mostly polymerase chain reaction, PCR) have been implemented. However, there are discrepancies in the reported accuracy of PCR.A systematic review with meta-analysis was conducted in order to evaluate the accuracy of PCR for the diagnosis of S. stercoralis infection. The protocol was registered with PROSPERO International Prospective Register of Systematic Reviews (record: CRD42016054298). Fourteen studies, 12 of which evaluating real-time PCR, were included in the analysis. The specificity of the techniques resulted high (ranging from 93 to 95%, according to the reference test(s) used). When all molecular techniques were compared to parasitological methods, the sensitivity of PCR was assessed at 71.8% (95% CI 52.2-85.5), that decreased to 61.8% (95% CI 42.0-78.4) when serology was added among the reference tests. Similarly, sensitivity of real-time PCR resulted 64.4% (95% CI 46.2-77.7) when compared to parasitological methods only, 56.5% (95% CI 39.2-72.4) including serology.PCR might not be suitable for screening purpose, whereas it might have a role as a confirmatory test. |
format |
Article in Journal/Newspaper |
author |
Dora Buonfrate Ana Requena-Mendez Andrea Angheben Michela Cinquini Mario Cruciani Andrea Fittipaldo Giovanni Giorli Federico Gobbi Chiara Piubelli Zeno Bisoffi |
author_facet |
Dora Buonfrate Ana Requena-Mendez Andrea Angheben Michela Cinquini Mario Cruciani Andrea Fittipaldo Giovanni Giorli Federico Gobbi Chiara Piubelli Zeno Bisoffi |
author_sort |
Dora Buonfrate |
title |
Accuracy of molecular biology techniques for the diagnosis of Strongyloides stercoralis infection-A systematic review and meta-analysis. |
title_short |
Accuracy of molecular biology techniques for the diagnosis of Strongyloides stercoralis infection-A systematic review and meta-analysis. |
title_full |
Accuracy of molecular biology techniques for the diagnosis of Strongyloides stercoralis infection-A systematic review and meta-analysis. |
title_fullStr |
Accuracy of molecular biology techniques for the diagnosis of Strongyloides stercoralis infection-A systematic review and meta-analysis. |
title_full_unstemmed |
Accuracy of molecular biology techniques for the diagnosis of Strongyloides stercoralis infection-A systematic review and meta-analysis. |
title_sort |
accuracy of molecular biology techniques for the diagnosis of strongyloides stercoralis infection-a systematic review and meta-analysis. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2018 |
url |
https://doi.org/10.1371/journal.pntd.0006229 https://doaj.org/article/7837700931cf4a00a01c24fa9839abe3 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 12, Iss 2, p e0006229 (2018) |
op_relation |
http://europepmc.org/articles/PMC5823464?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006229 https://doaj.org/article/7837700931cf4a00a01c24fa9839abe3 |
op_doi |
https://doi.org/10.1371/journal.pntd.0006229 |
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PLOS Neglected Tropical Diseases |
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12 |
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2 |
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e0006229 |
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1766340802948104192 |