Novel neuroprotective peptides in the venom of the solitary scoliid wasp Scolia decorata ventralis
Abstract Background Solitary wasp venoms may be a rich source of neuroactive substances, since their venoms are used for paralyzing preys. We have been exploring bioactive constituents of solitary wasp venoms and, in this study, the component profile of the venom from a solitary scoliid wasp, Scolia...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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Online Access: | https://doi.org/10.1590/1678-9199-jvatitd-2020-0171 https://doaj.org/article/7810f13e60dd4e9fb2b3f2b07adaa076 |
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ftdoajarticles:oai:doaj.org/article:7810f13e60dd4e9fb2b3f2b07adaa076 2023-05-15T15:16:52+02:00 Novel neuroprotective peptides in the venom of the solitary scoliid wasp Scolia decorata ventralis Carlos Alberto-Silva Fernanda Calheta Vieira Portaro Roberto Tadashi Kodama Halyne Queiroz Pantaleão Marisa Rangel Ken-ichi Nihei Katsuhiro Konno 2021-06-01T00:00:00Z https://doi.org/10.1590/1678-9199-jvatitd-2020-0171 https://doaj.org/article/7810f13e60dd4e9fb2b3f2b07adaa076 EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992021000100313&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1590/1678-9199-jvatitd-2020-0171 https://doaj.org/article/7810f13e60dd4e9fb2b3f2b07adaa076 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 27 (2021) Comprehensive analysis LC-ESI-MS Solitary wasp Venom Neuroprotective peptide Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2021 ftdoajarticles https://doi.org/10.1590/1678-9199-jvatitd-2020-0171 2022-12-31T15:15:13Z Abstract Background Solitary wasp venoms may be a rich source of neuroactive substances, since their venoms are used for paralyzing preys. We have been exploring bioactive constituents of solitary wasp venoms and, in this study, the component profile of the venom from a solitary scoliid wasp, Scolia decorata ventralis, was investigated through a comprehensive analysis using LC-MS. Two peptides were synthesized, and their neuroprotective properties were evaluated. Methods A reverse-phase HPLC connected to ESI-MS was used for LC-MS analyses. Online mass fingerprinting was performed from TIC, and data-dependent tandem mass spectrometry gave the MS/MS spectra. The sequences of two major peptide components were determined by MALDI-TOF/TOF MS analysis, confirmed by solid phase synthesis. Using the synthetic peptides, biological activities were assessed. Cell integrity tests and neuroprotection analyzes using H2O2 as an oxidative stress inducer were performed for both peptides. Results Online mass fingerprinting revealed that the venom contains 123 components, and the MS/MS analysis resulted in 33 full sequences of peptide components. The two main peptides, α-scoliidine (DYVTVKGFSPLR) and β-scoliidine (DYVTVKGFSPLRKA), present homology with the bradykinin C-terminal. Despite this, both peptides did not behave as substrates or inhibitors of ACE, indicating that they do not interact with this metallopeptidase. In further studies, β-scoliidine, but not α -scoliidine, showed protective effects against oxidative stress-induced neurotoxicity in PC12 cells through integrity and metabolism cell assays. Interestingly, β-scoliidine has the extension of the KA dipeptide at the C-terminal in comparison with α-scoliidine. Conclusion Comprehensive LC-MS and MS/MS analyses from the Scolia decorata ventralis venom displayed the component profile of this venom. β-scoliidine showed an effective cytoprotective effect, probably due to the observed increase in the number of cells. This is the first report of solitary wasp venom peptides ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 27 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Comprehensive analysis LC-ESI-MS Solitary wasp Venom Neuroprotective peptide Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
Comprehensive analysis LC-ESI-MS Solitary wasp Venom Neuroprotective peptide Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 Carlos Alberto-Silva Fernanda Calheta Vieira Portaro Roberto Tadashi Kodama Halyne Queiroz Pantaleão Marisa Rangel Ken-ichi Nihei Katsuhiro Konno Novel neuroprotective peptides in the venom of the solitary scoliid wasp Scolia decorata ventralis |
topic_facet |
Comprehensive analysis LC-ESI-MS Solitary wasp Venom Neuroprotective peptide Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Abstract Background Solitary wasp venoms may be a rich source of neuroactive substances, since their venoms are used for paralyzing preys. We have been exploring bioactive constituents of solitary wasp venoms and, in this study, the component profile of the venom from a solitary scoliid wasp, Scolia decorata ventralis, was investigated through a comprehensive analysis using LC-MS. Two peptides were synthesized, and their neuroprotective properties were evaluated. Methods A reverse-phase HPLC connected to ESI-MS was used for LC-MS analyses. Online mass fingerprinting was performed from TIC, and data-dependent tandem mass spectrometry gave the MS/MS spectra. The sequences of two major peptide components were determined by MALDI-TOF/TOF MS analysis, confirmed by solid phase synthesis. Using the synthetic peptides, biological activities were assessed. Cell integrity tests and neuroprotection analyzes using H2O2 as an oxidative stress inducer were performed for both peptides. Results Online mass fingerprinting revealed that the venom contains 123 components, and the MS/MS analysis resulted in 33 full sequences of peptide components. The two main peptides, α-scoliidine (DYVTVKGFSPLR) and β-scoliidine (DYVTVKGFSPLRKA), present homology with the bradykinin C-terminal. Despite this, both peptides did not behave as substrates or inhibitors of ACE, indicating that they do not interact with this metallopeptidase. In further studies, β-scoliidine, but not α -scoliidine, showed protective effects against oxidative stress-induced neurotoxicity in PC12 cells through integrity and metabolism cell assays. Interestingly, β-scoliidine has the extension of the KA dipeptide at the C-terminal in comparison with α-scoliidine. Conclusion Comprehensive LC-MS and MS/MS analyses from the Scolia decorata ventralis venom displayed the component profile of this venom. β-scoliidine showed an effective cytoprotective effect, probably due to the observed increase in the number of cells. This is the first report of solitary wasp venom peptides ... |
format |
Article in Journal/Newspaper |
author |
Carlos Alberto-Silva Fernanda Calheta Vieira Portaro Roberto Tadashi Kodama Halyne Queiroz Pantaleão Marisa Rangel Ken-ichi Nihei Katsuhiro Konno |
author_facet |
Carlos Alberto-Silva Fernanda Calheta Vieira Portaro Roberto Tadashi Kodama Halyne Queiroz Pantaleão Marisa Rangel Ken-ichi Nihei Katsuhiro Konno |
author_sort |
Carlos Alberto-Silva |
title |
Novel neuroprotective peptides in the venom of the solitary scoliid wasp Scolia decorata ventralis |
title_short |
Novel neuroprotective peptides in the venom of the solitary scoliid wasp Scolia decorata ventralis |
title_full |
Novel neuroprotective peptides in the venom of the solitary scoliid wasp Scolia decorata ventralis |
title_fullStr |
Novel neuroprotective peptides in the venom of the solitary scoliid wasp Scolia decorata ventralis |
title_full_unstemmed |
Novel neuroprotective peptides in the venom of the solitary scoliid wasp Scolia decorata ventralis |
title_sort |
novel neuroprotective peptides in the venom of the solitary scoliid wasp scolia decorata ventralis |
publisher |
SciELO |
publishDate |
2021 |
url |
https://doi.org/10.1590/1678-9199-jvatitd-2020-0171 https://doaj.org/article/7810f13e60dd4e9fb2b3f2b07adaa076 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 27 (2021) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992021000100313&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1590/1678-9199-jvatitd-2020-0171 https://doaj.org/article/7810f13e60dd4e9fb2b3f2b07adaa076 |
op_doi |
https://doi.org/10.1590/1678-9199-jvatitd-2020-0171 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
27 |
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1766347170286403584 |