Serotype and mating type characterization of Cryptococcus neoformans by Multiplex PCR Caracterização de sorotipo e "mating type" de Cryptococcus neoformans por PCR Multiplex
Cryptococcus neoformans is an encapsulated yeast, etiological agent of cryptococcosis. The species is commonly associated with pigeon droppings and plant materials. The aim of the present work was to verify the presence of the yeast in pigeon droppings, and to identify the isolates obtained in serot...
Published in: | Revista do Instituto de Medicina Tropical de São Paulo |
---|---|
Main Authors: | , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Universidade de São Paulo (USP)
2007
|
Subjects: | |
Online Access: | https://doi.org/10.1590/S0036-46652007000400002 https://doaj.org/article/780ebf481edd45b9a81917527c434b57 |
id |
ftdoajarticles:oai:doaj.org/article:780ebf481edd45b9a81917527c434b57 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:780ebf481edd45b9a81917527c434b57 2024-09-09T19:28:30+00:00 Serotype and mating type characterization of Cryptococcus neoformans by Multiplex PCR Caracterização de sorotipo e "mating type" de Cryptococcus neoformans por PCR Multiplex Vívian Gonçalves Carvalho Mateus Souza Terceti Amanda Latercia Tranches Dias Claudete Rodrigues Paula Juliana Pereira Lyon Antônio Martins de Siqueira Marília Caixeta Franco 2007-08-01T00:00:00Z https://doi.org/10.1590/S0036-46652007000400002 https://doaj.org/article/780ebf481edd45b9a81917527c434b57 EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652007000400002 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46652007000400002 0036-4665 1678-9946 https://doaj.org/article/780ebf481edd45b9a81917527c434b57 Revista do Instituto de Medicina Tropical de São Paulo, Vol 49, Iss 4, Pp 207-210 (2007) Cryptococcus neoformans Serotype Mating Type PCR Kit Crypto Check® Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2007 ftdoajarticles https://doi.org/10.1590/S0036-46652007000400002 2024-08-05T17:49:31Z Cryptococcus neoformans is an encapsulated yeast, etiological agent of cryptococcosis. The species is commonly associated with pigeon droppings and plant materials. The aim of the present work was to verify the presence of the yeast in pigeon droppings, and to identify the isolates obtained in serotypes and mating types (MAT). Ten samples of pigeon droppings were collected in the rural area of the city of Alfenas, Brazil. Samples were inoculated in agar Niger medium for fungal isolation and 22 isolates with characteristics of C. neoformans were obtained. The serotypes and MAT were determined by multiplex PCR using specific primers. Serotypes were also determined by using the Kit Crypto Check. Among the 22 samples evaluated, eight were identified as C. neoformans by classic identification tests. These samples were characterized as serotype A by the Kit Crypto check and as serotype A MAT alpha by the multiplex PCR. The present study reinforces the evidence that pigeon droppings are a reservoir for C. neoformans and confirms the prevalence of C. neoformans var. grubii (Aalpha) among environmental isolates. It also demonstrates that multiplex PCR is an acceptable alternative for serotype analysis because it reduces the costs for each reaction and analyses serotype and MAT simultaneously. Cryptococcus neoformans é levedura encapsulada, agente etiológico da criptococose. As espécies são comumente associadas com fezes de pombos e material vegetal. O objetivo do presente trabalho foi verificar a presença de leveduras em fezes de pombos e identificar os isolados em relação aos sorotipos e "mating types". Dez amostras de fezes de pombos foram coletadas na zona rural da cidade de Alfenas, Brasil. As amostras foram inoculadas em agar Niger e 22 isolados com características de C. neoformans foram obtidos. Os sorotipos e "mating types" foram determinados pela PCR multiplex e os sorotipos foram identificados também pelo Kit Crypto Check. Dentre as 22 amostras avaliadas, oito foram identificadas como C. neoformans através dos ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista do Instituto de Medicina Tropical de São Paulo 49 4 207 210 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Cryptococcus neoformans Serotype Mating Type PCR Kit Crypto Check® Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Cryptococcus neoformans Serotype Mating Type PCR Kit Crypto Check® Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Vívian Gonçalves Carvalho Mateus Souza Terceti Amanda Latercia Tranches Dias Claudete Rodrigues Paula Juliana Pereira Lyon Antônio Martins de Siqueira Marília Caixeta Franco Serotype and mating type characterization of Cryptococcus neoformans by Multiplex PCR Caracterização de sorotipo e "mating type" de Cryptococcus neoformans por PCR Multiplex |
topic_facet |
Cryptococcus neoformans Serotype Mating Type PCR Kit Crypto Check® Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Cryptococcus neoformans is an encapsulated yeast, etiological agent of cryptococcosis. The species is commonly associated with pigeon droppings and plant materials. The aim of the present work was to verify the presence of the yeast in pigeon droppings, and to identify the isolates obtained in serotypes and mating types (MAT). Ten samples of pigeon droppings were collected in the rural area of the city of Alfenas, Brazil. Samples were inoculated in agar Niger medium for fungal isolation and 22 isolates with characteristics of C. neoformans were obtained. The serotypes and MAT were determined by multiplex PCR using specific primers. Serotypes were also determined by using the Kit Crypto Check. Among the 22 samples evaluated, eight were identified as C. neoformans by classic identification tests. These samples were characterized as serotype A by the Kit Crypto check and as serotype A MAT alpha by the multiplex PCR. The present study reinforces the evidence that pigeon droppings are a reservoir for C. neoformans and confirms the prevalence of C. neoformans var. grubii (Aalpha) among environmental isolates. It also demonstrates that multiplex PCR is an acceptable alternative for serotype analysis because it reduces the costs for each reaction and analyses serotype and MAT simultaneously. Cryptococcus neoformans é levedura encapsulada, agente etiológico da criptococose. As espécies são comumente associadas com fezes de pombos e material vegetal. O objetivo do presente trabalho foi verificar a presença de leveduras em fezes de pombos e identificar os isolados em relação aos sorotipos e "mating types". Dez amostras de fezes de pombos foram coletadas na zona rural da cidade de Alfenas, Brasil. As amostras foram inoculadas em agar Niger e 22 isolados com características de C. neoformans foram obtidos. Os sorotipos e "mating types" foram determinados pela PCR multiplex e os sorotipos foram identificados também pelo Kit Crypto Check. Dentre as 22 amostras avaliadas, oito foram identificadas como C. neoformans através dos ... |
format |
Article in Journal/Newspaper |
author |
Vívian Gonçalves Carvalho Mateus Souza Terceti Amanda Latercia Tranches Dias Claudete Rodrigues Paula Juliana Pereira Lyon Antônio Martins de Siqueira Marília Caixeta Franco |
author_facet |
Vívian Gonçalves Carvalho Mateus Souza Terceti Amanda Latercia Tranches Dias Claudete Rodrigues Paula Juliana Pereira Lyon Antônio Martins de Siqueira Marília Caixeta Franco |
author_sort |
Vívian Gonçalves Carvalho |
title |
Serotype and mating type characterization of Cryptococcus neoformans by Multiplex PCR Caracterização de sorotipo e "mating type" de Cryptococcus neoformans por PCR Multiplex |
title_short |
Serotype and mating type characterization of Cryptococcus neoformans by Multiplex PCR Caracterização de sorotipo e "mating type" de Cryptococcus neoformans por PCR Multiplex |
title_full |
Serotype and mating type characterization of Cryptococcus neoformans by Multiplex PCR Caracterização de sorotipo e "mating type" de Cryptococcus neoformans por PCR Multiplex |
title_fullStr |
Serotype and mating type characterization of Cryptococcus neoformans by Multiplex PCR Caracterização de sorotipo e "mating type" de Cryptococcus neoformans por PCR Multiplex |
title_full_unstemmed |
Serotype and mating type characterization of Cryptococcus neoformans by Multiplex PCR Caracterização de sorotipo e "mating type" de Cryptococcus neoformans por PCR Multiplex |
title_sort |
serotype and mating type characterization of cryptococcus neoformans by multiplex pcr caracterização de sorotipo e "mating type" de cryptococcus neoformans por pcr multiplex |
publisher |
Universidade de São Paulo (USP) |
publishDate |
2007 |
url |
https://doi.org/10.1590/S0036-46652007000400002 https://doaj.org/article/780ebf481edd45b9a81917527c434b57 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 49, Iss 4, Pp 207-210 (2007) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652007000400002 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46652007000400002 0036-4665 1678-9946 https://doaj.org/article/780ebf481edd45b9a81917527c434b57 |
op_doi |
https://doi.org/10.1590/S0036-46652007000400002 |
container_title |
Revista do Instituto de Medicina Tropical de São Paulo |
container_volume |
49 |
container_issue |
4 |
container_start_page |
207 |
op_container_end_page |
210 |
_version_ |
1809897827580510208 |