Developmental transcriptomic features of the carcinogenic liver fluke, Clonorchis sinensis.
Clonorchis sinensis is the causative agent of the life-threatening disease endemic to China, Korea, and Vietnam. It is estimated that about 15 million people are infected with this fluke. C. sinensis provokes inflammation, epithelial hyperplasia, and periductal fibrosis in bile ducts, and may cause...
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ftdoajarticles:oai:doaj.org/article:77ffebc058104d17b9401f7fb8c95ae3 2023-05-15T15:16:50+02:00 Developmental transcriptomic features of the carcinogenic liver fluke, Clonorchis sinensis. Won Gi Yoo Dae-Won Kim Jung-Won Ju Pyo Yun Cho Tae Im Kim Shin-Hyeong Cho Sang-Haeng Choi Hong-Seog Park Tong-Soo Kim Sung-Jong Hong 2011-06-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0001208 https://doaj.org/article/77ffebc058104d17b9401f7fb8c95ae3 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3125140?pdf=render https://doaj.org/toc/1935-2735 1935-2735 doi:10.1371/journal.pntd.0001208 https://doaj.org/article/77ffebc058104d17b9401f7fb8c95ae3 PLoS Neglected Tropical Diseases, Vol 5, Iss 6, p e1208 (2011) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2011 ftdoajarticles https://doi.org/10.1371/journal.pntd.0001208 2022-12-31T03:20:18Z Clonorchis sinensis is the causative agent of the life-threatening disease endemic to China, Korea, and Vietnam. It is estimated that about 15 million people are infected with this fluke. C. sinensis provokes inflammation, epithelial hyperplasia, and periductal fibrosis in bile ducts, and may cause cholangiocarcinoma in chronically infected individuals. Accumulation of a large amount of biological information about the adult stage of this liver fluke in recent years has advanced our understanding of the pathological interplay between this parasite and its hosts. However, no developmental gene expression profiles of C. sinensis have been published. In this study, we generated gene expression profiles of three developmental stages of C. sinensis by analyzing expressed sequence tags (ESTs). Complementary DNA libraries were constructed from the adult, metacercaria, and egg developmental stages of C. sinensis. A total of 52,745 ESTs were generated and assembled into 12,830 C. sinensis assembled EST sequences, and then these assemblies were further categorized into groups according to biological functions and developmental stages. Most of the genes that were differentially expressed in the different stages were consistent with the biological and physical features of the particular developmental stage; high energy metabolism, motility and reproduction genes were differentially expressed in adults, minimal metabolism and final host adaptation genes were differentially expressed in metacercariae, and embryonic genes were differentially expressed in eggs. The higher expression of glucose transporters, proteases, and antioxidant enzymes in the adults accounts for active uptake of nutrients and defense against host immune attacks. The types of ion channels present in C. sinensis are consistent with its parasitic nature and phylogenetic placement in the tree of life. We anticipate that the transcriptomic information on essential regulators of development, bile chemotaxis, and physico-metabolic pathways in C. sinensis that ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 5 6 e1208 |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Won Gi Yoo Dae-Won Kim Jung-Won Ju Pyo Yun Cho Tae Im Kim Shin-Hyeong Cho Sang-Haeng Choi Hong-Seog Park Tong-Soo Kim Sung-Jong Hong Developmental transcriptomic features of the carcinogenic liver fluke, Clonorchis sinensis. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Clonorchis sinensis is the causative agent of the life-threatening disease endemic to China, Korea, and Vietnam. It is estimated that about 15 million people are infected with this fluke. C. sinensis provokes inflammation, epithelial hyperplasia, and periductal fibrosis in bile ducts, and may cause cholangiocarcinoma in chronically infected individuals. Accumulation of a large amount of biological information about the adult stage of this liver fluke in recent years has advanced our understanding of the pathological interplay between this parasite and its hosts. However, no developmental gene expression profiles of C. sinensis have been published. In this study, we generated gene expression profiles of three developmental stages of C. sinensis by analyzing expressed sequence tags (ESTs). Complementary DNA libraries were constructed from the adult, metacercaria, and egg developmental stages of C. sinensis. A total of 52,745 ESTs were generated and assembled into 12,830 C. sinensis assembled EST sequences, and then these assemblies were further categorized into groups according to biological functions and developmental stages. Most of the genes that were differentially expressed in the different stages were consistent with the biological and physical features of the particular developmental stage; high energy metabolism, motility and reproduction genes were differentially expressed in adults, minimal metabolism and final host adaptation genes were differentially expressed in metacercariae, and embryonic genes were differentially expressed in eggs. The higher expression of glucose transporters, proteases, and antioxidant enzymes in the adults accounts for active uptake of nutrients and defense against host immune attacks. The types of ion channels present in C. sinensis are consistent with its parasitic nature and phylogenetic placement in the tree of life. We anticipate that the transcriptomic information on essential regulators of development, bile chemotaxis, and physico-metabolic pathways in C. sinensis that ... |
format |
Article in Journal/Newspaper |
author |
Won Gi Yoo Dae-Won Kim Jung-Won Ju Pyo Yun Cho Tae Im Kim Shin-Hyeong Cho Sang-Haeng Choi Hong-Seog Park Tong-Soo Kim Sung-Jong Hong |
author_facet |
Won Gi Yoo Dae-Won Kim Jung-Won Ju Pyo Yun Cho Tae Im Kim Shin-Hyeong Cho Sang-Haeng Choi Hong-Seog Park Tong-Soo Kim Sung-Jong Hong |
author_sort |
Won Gi Yoo |
title |
Developmental transcriptomic features of the carcinogenic liver fluke, Clonorchis sinensis. |
title_short |
Developmental transcriptomic features of the carcinogenic liver fluke, Clonorchis sinensis. |
title_full |
Developmental transcriptomic features of the carcinogenic liver fluke, Clonorchis sinensis. |
title_fullStr |
Developmental transcriptomic features of the carcinogenic liver fluke, Clonorchis sinensis. |
title_full_unstemmed |
Developmental transcriptomic features of the carcinogenic liver fluke, Clonorchis sinensis. |
title_sort |
developmental transcriptomic features of the carcinogenic liver fluke, clonorchis sinensis. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2011 |
url |
https://doi.org/10.1371/journal.pntd.0001208 https://doaj.org/article/77ffebc058104d17b9401f7fb8c95ae3 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 5, Iss 6, p e1208 (2011) |
op_relation |
http://europepmc.org/articles/PMC3125140?pdf=render https://doaj.org/toc/1935-2735 1935-2735 doi:10.1371/journal.pntd.0001208 https://doaj.org/article/77ffebc058104d17b9401f7fb8c95ae3 |
op_doi |
https://doi.org/10.1371/journal.pntd.0001208 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
5 |
container_issue |
6 |
container_start_page |
e1208 |
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1766347131371651072 |