Bioactivity Screening of Antarctic Sponges Reveals Anticancer Activity and Potential Cell Death via Ferroptosis by Mycalols
Sponges are known to produce a series of compounds with bioactivities useful for human health. This study was conducted on four sponges collected in the framework of the XXXIV Italian National Antarctic Research Program (PNRA) in November-December 2018, i.e., Mycale (Oxymycale) acerata , Haliclona (...
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ftdoajarticles:oai:doaj.org/article:77d8d3b1345b479abafe560bd79330ca 2023-05-15T13:45:14+02:00 Bioactivity Screening of Antarctic Sponges Reveals Anticancer Activity and Potential Cell Death via Ferroptosis by Mycalols Gennaro Riccio Genoveffa Nuzzo Gianluca Zazo Daniela Coppola Giuseppina Senese Lucia Romano Maria Costantini Nadia Ruocco Marco Bertolino Angelo Fontana Adrianna Ianora Cinzia Verde Daniela Giordano Chiara Lauritano 2021-08-01T00:00:00Z https://doi.org/10.3390/md19080459 https://doaj.org/article/77d8d3b1345b479abafe560bd79330ca EN eng MDPI AG https://www.mdpi.com/1660-3397/19/8/459 https://doaj.org/toc/1660-3397 doi:10.3390/md19080459 1660-3397 https://doaj.org/article/77d8d3b1345b479abafe560bd79330ca Marine Drugs, Vol 19, Iss 459, p 459 (2021) Antarctica sponges drug discovery mycalols marine biotechnology Biology (General) QH301-705.5 article 2021 ftdoajarticles https://doi.org/10.3390/md19080459 2022-12-30T23:37:53Z Sponges are known to produce a series of compounds with bioactivities useful for human health. This study was conducted on four sponges collected in the framework of the XXXIV Italian National Antarctic Research Program (PNRA) in November-December 2018, i.e., Mycale (Oxymycale) acerata , Haliclona (Rhizoniera) dancoi , Hemimycale topsenti , and Hemigellius pilosus . Sponge extracts were fractioned and tested against hepatocellular carcinoma (HepG2), lung carcinoma (A549), and melanoma cells (A2058), in order to screen for antiproliferative or cytotoxic activity. Two different chemical classes of compounds, belonging to mycalols and suberitenones, were identified in the active fractions. Mycalols were the most active compounds, and their mechanism of action was also investigated at the gene and protein levels in HepG2 cells. Of the differentially expressed genes, ULK1 and GALNT5 were the most down-regulated genes, while MAPK8 was one of the most up-regulated genes. These genes were previously associated with ferroptosis, a programmed cell death triggered by iron-dependent lipid peroxidation, confirmed at the protein level by the down-regulation of GPX4, a key regulator of ferroptosis, and the up-regulation of NCOA4, involved in iron homeostasis. These data suggest, for the first time, that mycalols act by triggering ferroptosis in HepG2 cells. Article in Journal/Newspaper Antarc* Antarctic Antarctica Italian National Antarctic Research Program National Antarctic Research Program Directory of Open Access Journals: DOAJ Articles Antarctic Marine Drugs 19 8 459 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Antarctica sponges drug discovery mycalols marine biotechnology Biology (General) QH301-705.5 |
spellingShingle |
Antarctica sponges drug discovery mycalols marine biotechnology Biology (General) QH301-705.5 Gennaro Riccio Genoveffa Nuzzo Gianluca Zazo Daniela Coppola Giuseppina Senese Lucia Romano Maria Costantini Nadia Ruocco Marco Bertolino Angelo Fontana Adrianna Ianora Cinzia Verde Daniela Giordano Chiara Lauritano Bioactivity Screening of Antarctic Sponges Reveals Anticancer Activity and Potential Cell Death via Ferroptosis by Mycalols |
topic_facet |
Antarctica sponges drug discovery mycalols marine biotechnology Biology (General) QH301-705.5 |
description |
Sponges are known to produce a series of compounds with bioactivities useful for human health. This study was conducted on four sponges collected in the framework of the XXXIV Italian National Antarctic Research Program (PNRA) in November-December 2018, i.e., Mycale (Oxymycale) acerata , Haliclona (Rhizoniera) dancoi , Hemimycale topsenti , and Hemigellius pilosus . Sponge extracts were fractioned and tested against hepatocellular carcinoma (HepG2), lung carcinoma (A549), and melanoma cells (A2058), in order to screen for antiproliferative or cytotoxic activity. Two different chemical classes of compounds, belonging to mycalols and suberitenones, were identified in the active fractions. Mycalols were the most active compounds, and their mechanism of action was also investigated at the gene and protein levels in HepG2 cells. Of the differentially expressed genes, ULK1 and GALNT5 were the most down-regulated genes, while MAPK8 was one of the most up-regulated genes. These genes were previously associated with ferroptosis, a programmed cell death triggered by iron-dependent lipid peroxidation, confirmed at the protein level by the down-regulation of GPX4, a key regulator of ferroptosis, and the up-regulation of NCOA4, involved in iron homeostasis. These data suggest, for the first time, that mycalols act by triggering ferroptosis in HepG2 cells. |
format |
Article in Journal/Newspaper |
author |
Gennaro Riccio Genoveffa Nuzzo Gianluca Zazo Daniela Coppola Giuseppina Senese Lucia Romano Maria Costantini Nadia Ruocco Marco Bertolino Angelo Fontana Adrianna Ianora Cinzia Verde Daniela Giordano Chiara Lauritano |
author_facet |
Gennaro Riccio Genoveffa Nuzzo Gianluca Zazo Daniela Coppola Giuseppina Senese Lucia Romano Maria Costantini Nadia Ruocco Marco Bertolino Angelo Fontana Adrianna Ianora Cinzia Verde Daniela Giordano Chiara Lauritano |
author_sort |
Gennaro Riccio |
title |
Bioactivity Screening of Antarctic Sponges Reveals Anticancer Activity and Potential Cell Death via Ferroptosis by Mycalols |
title_short |
Bioactivity Screening of Antarctic Sponges Reveals Anticancer Activity and Potential Cell Death via Ferroptosis by Mycalols |
title_full |
Bioactivity Screening of Antarctic Sponges Reveals Anticancer Activity and Potential Cell Death via Ferroptosis by Mycalols |
title_fullStr |
Bioactivity Screening of Antarctic Sponges Reveals Anticancer Activity and Potential Cell Death via Ferroptosis by Mycalols |
title_full_unstemmed |
Bioactivity Screening of Antarctic Sponges Reveals Anticancer Activity and Potential Cell Death via Ferroptosis by Mycalols |
title_sort |
bioactivity screening of antarctic sponges reveals anticancer activity and potential cell death via ferroptosis by mycalols |
publisher |
MDPI AG |
publishDate |
2021 |
url |
https://doi.org/10.3390/md19080459 https://doaj.org/article/77d8d3b1345b479abafe560bd79330ca |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctica Italian National Antarctic Research Program National Antarctic Research Program |
genre_facet |
Antarc* Antarctic Antarctica Italian National Antarctic Research Program National Antarctic Research Program |
op_source |
Marine Drugs, Vol 19, Iss 459, p 459 (2021) |
op_relation |
https://www.mdpi.com/1660-3397/19/8/459 https://doaj.org/toc/1660-3397 doi:10.3390/md19080459 1660-3397 https://doaj.org/article/77d8d3b1345b479abafe560bd79330ca |
op_doi |
https://doi.org/10.3390/md19080459 |
container_title |
Marine Drugs |
container_volume |
19 |
container_issue |
8 |
container_start_page |
459 |
_version_ |
1766217606522470400 |